Journal: Cardiovascular Research

Article Title: STAT3-miR-17/20 signalling axis plays a critical role in attenuating myocardial infarction following rapamycin treatment in diabetic mice

doi: 10.1093/cvr/cvz315

Figure Lengend Snippet: Role of STAT3 in hearts of diabetic mice following rapamycin treatment. (A) Representative immunoblots for p-STAT3, total STAT3, and GAPDH expression in whole heart of C57 and db/db mice following 28 days of RAPA treatment. Densitometry analysis of immunoblots for the ratio of p-STAT3/STAT3 (*P < 0.05 vs. others) and the ratio of STAT3/GAPDH (*P < 0.05 vs. C57-Control; n = 3). (B) Myocardial infarct size following ischaemia/reperfusion in STAT3-deficient diabetic mice. High-fat diet (HFD)-fed WT and STAT3-deficient mice were treated with RAPA for 28 days prior to evaluation of I/R injury. Upper panel: representative images of heart sections following TTC staining (Scale indicates 5 mm). Lower panels: quantitative data of infarct size following I/R injury (*P < 0.001 vs. other) and rate-force product (*P < 0.01 vs. other; n = 5). (C) Isolated cardiomyocyte necrosis was determined by trypan blue staining following 40 min SI and 1 h RO (n = 4–8). (D) Representative pictures of TUNEL staining (scale indicates 100 µm) and quantitative data of cardiomyocyte apoptosis following 40 min SI and 18 h RO (n = 4; *P < 0.001 vs. controls, αP < 0.001 vs. both SI/RO and βP < 0.05 vs. WT-SI/RO). Statistics: one-way ANOVA.

Article Snippet: 23 Apoptosis was determined after 40 min SI and 18 h of RO using TUNEL staining kit (BD Biosciences, San Jose, CA, USA) as previously reported.

Techniques: Western Blot, Expressing, Staining, Isolation, TUNEL Assay

Journal: Scientific Reports

Article Title: Validation of MRI quantitative susceptibility mapping of superparamagnetic iron oxide nanoparticles for hyperthermia applications in live subjects

doi: 10.1038/s41598-020-58219-9

Figure Lengend Snippet: Histological examination to confirm hyperthermia-induced tumor killing. ( a ) Adjacent tumor sections were stained for Prussian blue to detect iron, GFP to detect viable tumor cells, and TUNEL to detect apoptotic cells. Tumor slices were obtained from four cohorts of ferumoxytol/AMF combinations. Red asterisk is placed to a region of Prussian blue positive and TUNEL negative regions. Yellow asterisk is placed to a region of Prussian blue positive and TUNEL positive. ( b ) Zoom-in view of the slice of tumor from +/+ of ferumoxytol/AMF cohort. Green circle indicates the site of intratumorally injected ferumoxytol. Blue circle is placed on tumor stroma (GFP negative) that stained positive both for Prussian blue and TUNEL.

Article Snippet: Sections were stained for iron accumulation by Prussian Blue staining , to detect DNA breaks by TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) stain, and to localize tumor growth by GFP immunostaining (performed by Histowiz, Inc.).

Techniques: Staining, TUNEL Assay, Injection