Journal: PLoS ONE

Article Title: Bilateral Nerve Alterations in a Unilateral Experimental Neurotrophic Keratopathy Model: A Lateral Conjunctival Approach for Trigeminal Axotomy

doi: 10.1371/journal.pone.0070908

Figure Lengend Snippet: Representative histological photographs of corneal nerves stained with anti-βIII tubulin FITC-conjugated antibody. Normal subbasal (A) and stromal (E) nerve plexus. Subbasal nerve plexus completely disappeared (B; day 1, C; day 7, D; day 14) and stromal nerve definitely decreased from day1 (F; day 1, G; day 7, H; day 14) and after trigeminal axotomy. The nerve of the contralateral eye decreased from day 1 (J; day 1, K; day 7, L; day 14).

Article Snippet: Corneas were then stained with monoclonal NL637-conjugated anti-β-III tubulin antibody (anti-Neuron-specific β-III Tubulin-NL637, R&D systems Inc. Minneapolis, MN; dilution of 1∶100) at 4C degree overnight.

Techniques: Staining

Journal: PLoS ONE

Article Title: Bilateral Nerve Alterations in a Unilateral Experimental Neurotrophic Keratopathy Model: A Lateral Conjunctival Approach for Trigeminal Axotomy

doi: 10.1371/journal.pone.0070908

Figure Lengend Snippet: Representative histological photographs of corneal nerves stained with anti-βIII tubulin FITC-conjugated antibody. Normal subbasal (A) and stromal (E) nerve plexus.Subbasal nerve plexus completely disappeared (B; day 1, C; day 7, D; day 14) (A) and stromal nerve definitely decreased (B) from day 1 (F; day 1, G; day 7, H; day 14) after trigeminal axotomy. The nerve of the contralateral eye did not change (J; day 1, K; day 7, L; day 14).

Article Snippet: Corneas were then stained with monoclonal NL637-conjugated anti-β-III tubulin antibody (anti-Neuron-specific β-III Tubulin-NL637, R&D systems Inc. Minneapolis, MN; dilution of 1∶100) at 4C degree overnight.

Techniques: Staining

Journal: Toxicological Sciences

Article Title: Editor’s Highlight: Multiparametric Image Analysis of Rat Dorsal Root Ganglion Cultures to Evaluate Peripheral Neuropathy-Inducing Chemotherapeutics

doi: 10.1093/toxsci/kfw254

Figure Lengend Snippet: Tuj-1 and vimentin as specific markers for neuronal and non-neuronal DRG cells in culture, respectively. A, Full-length image of a Wes™ Simple Western blot for Tuj-1, vimentin, and GAPDH). The whole cell lysates were collected from untreated cells on Day 8 (72 h time point); lysates loaded in each capillary at 80 pg protein and probed with anti-tuj-1 (MO15013 1:50, plus anti-GAPDH 1:100) or anti-vimentin (AB8069 1:1000, plus anti-GAPDH 1:100); Tuj-1, Vimentin and GAPDH were detected at 55, 58 and 42 kDa, respectively. B, Tuj-1 and vimentin expression normalized to GAPDH (mean ± SEM, n = 3–4). C, A representative field of four channels; merged images taken with 20× objective from a vehicle control well. Neurons, neurites and non-neuronal cells, processes are stained positive for Tuj-1 (in red) and Vimentin (in green), respectively; Closed arrows point to the neuronal cell body (Tuj-1 in red) and nuclei (NeuN in white); Open arrows point to running of a single thread of neurite and process in close contact; Stars denote cells with a unique morphology distinctive from Schwann cells.

Article Snippet: The primary antibodies were diluted with antibody diluent (ProteinSimple) at 1:50 for Tuj-1 (Neuromics, MO15013) and SOX2 (Abcam, ab137385), 1:1000 for vimentin (Abcam, ab8069), 1:25 for glutamine synthetase (EMD Millipore, MAB302).

Techniques: Western Blot, Expressing, Staining

Journal: Toxicological Sciences

Article Title: Editor’s Highlight: Multiparametric Image Analysis of Rat Dorsal Root Ganglion Cultures to Evaluate Peripheral Neuropathy-Inducing Chemotherapeutics

doi: 10.1093/toxsci/kfw254

Figure Lengend Snippet: Image processing for multiparametric analysis with IN Cell Workstation software. A and B, Fluorescence images merged with NeuN and Tuj-1 channels or DAPI and vimentin channels, respectively; C and D, Show segmentation of nuclei (circled in blue lines), cell bodies (circled in green lines) and neurites or non-neuronal cell processes (outlined in yellow lines). Arrows in each image point to identical nuclei of neuronal cells (A and C) or non-neuronal cells. Scale bar = 40 µm.

Article Snippet: The primary antibodies were diluted with antibody diluent (ProteinSimple) at 1:50 for Tuj-1 (Neuromics, MO15013) and SOX2 (Abcam, ab137385), 1:1000 for vimentin (Abcam, ab8069), 1:25 for glutamine synthetase (EMD Millipore, MAB302).

Techniques: Software, Fluorescence

Journal: Neuron

Article Title: Driving axon regeneration by orchestrating neuronal and non-neuronal innate immune responses via the IFNγ-cGAS-STING axis

doi: 10.1016/j.neuron.2022.10.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Chicken anti-Tuj1 , Aves Labs , Cat#TUJ; RRID:AB_2313564.

Techniques: Control, Virus, Plasmid Preparation, Recombinant, RNAscope, Multiplex Assay, Fluorescence, Reverse Transcription, SYBR Green Assay, Single Cell Gel Electrophoresis, Knock-Out, Knock-In, shRNA, Sequencing, Software