ttr Search Results


mycoplasma fermentans strain pg  (ATCC)
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ATCC mycoplasma fermentans strain pg
Mycoplasma Fermentans Strain Pg, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp ttr mm00443267 m1  (Thermo Fisher)
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Thermo Fisher gene exp ttr mm00443267 m1
Gene Exp Ttr Mm00443267 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti ttr  (Proteintech)
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Proteintech anti ttr
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elisa kit  (Boster Bio)
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Boster Bio elisa kit
Reagents used in this research.
Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inducible system vector plvet ttr krab  (Addgene inc)
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Addgene inc inducible system vector plvet ttr krab
Reagents used in this research.
Inducible System Vector Plvet Ttr Krab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shrna lentiviral vectors plvct ttr krab  (Addgene inc)
93
Addgene inc shrna lentiviral vectors plvct ttr krab
Figure 4. KD of Sulf-2 expression in MYCN-A neuroblastoma cells results in loss of viability. SK-N-BE(2) and NB-19 neuroblastoma cells were transfected with <t>siRNA</t> for Sulf-2 and examined for survival and proliferation. A, Western blot analysis of Sulf-2 expression on cell lysates obtained 48 hours after transfection. B, representative crystal violet stains of cells in culture 48 hours after transfection [top, SK-N-BE(2); bottom, NB-19]. C, cell viability was measured at indicated time. Data, mean (SD) fluorescence units (RLU) of quadruplicate samples and are representative of three separate experiments showing similar results; , P < 0.05; , P < 0.01; , P < 0.0001. D, BrdUrd and PI analysis of NB-19 cells by flow cytometry. The data are representative of two experiments showing similar results. Top, cells transfected with SCR; bottom, cells transfected with siRNA 1 þ 2. E, bar diagram of the distribution of cells in phases of cell cycle according to PI content from the experiments shown in D.
Shrna Lentiviral Vectors Plvct Ttr Krab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp ttr hs00174914 m1  (Thermo Fisher)
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Thermo Fisher gene exp ttr hs00174914 m1
Figure 4. KD of Sulf-2 expression in MYCN-A neuroblastoma cells results in loss of viability. SK-N-BE(2) and NB-19 neuroblastoma cells were transfected with <t>siRNA</t> for Sulf-2 and examined for survival and proliferation. A, Western blot analysis of Sulf-2 expression on cell lysates obtained 48 hours after transfection. B, representative crystal violet stains of cells in culture 48 hours after transfection [top, SK-N-BE(2); bottom, NB-19]. C, cell viability was measured at indicated time. Data, mean (SD) fluorescence units (RLU) of quadruplicate samples and are representative of three separate experiments showing similar results; , P < 0.05; , P < 0.01; , P < 0.0001. D, BrdUrd and PI analysis of NB-19 cells by flow cytometry. The data are representative of two experiments showing similar results. Top, cells transfected with SCR; bottom, cells transfected with siRNA 1 þ 2. E, bar diagram of the distribution of cells in phases of cell cycle according to PI content from the experiments shown in D.
Gene Exp Ttr Hs00174914 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plvpt ttr krab  (Addgene inc)
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Addgene inc plvpt ttr krab
Figure 4. KD of Sulf-2 expression in MYCN-A neuroblastoma cells results in loss of viability. SK-N-BE(2) and NB-19 neuroblastoma cells were transfected with <t>siRNA</t> for Sulf-2 and examined for survival and proliferation. A, Western blot analysis of Sulf-2 expression on cell lysates obtained 48 hours after transfection. B, representative crystal violet stains of cells in culture 48 hours after transfection [top, SK-N-BE(2); bottom, NB-19]. C, cell viability was measured at indicated time. Data, mean (SD) fluorescence units (RLU) of quadruplicate samples and are representative of three separate experiments showing similar results; , P < 0.05; , P < 0.01; , P < 0.0001. D, BrdUrd and PI analysis of NB-19 cells by flow cytometry. The data are representative of two experiments showing similar results. Top, cells transfected with SCR; bottom, cells transfected with siRNA 1 þ 2. E, bar diagram of the distribution of cells in phases of cell cycle according to PI content from the experiments shown in D.
Plvpt Ttr Krab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assaymax human prealbumin elisa kit  (Assaypro)
96
Assaypro assaymax human prealbumin elisa kit
Figure 4. KD of Sulf-2 expression in MYCN-A neuroblastoma cells results in loss of viability. SK-N-BE(2) and NB-19 neuroblastoma cells were transfected with <t>siRNA</t> for Sulf-2 and examined for survival and proliferation. A, Western blot analysis of Sulf-2 expression on cell lysates obtained 48 hours after transfection. B, representative crystal violet stains of cells in culture 48 hours after transfection [top, SK-N-BE(2); bottom, NB-19]. C, cell viability was measured at indicated time. Data, mean (SD) fluorescence units (RLU) of quadruplicate samples and are representative of three separate experiments showing similar results; , P < 0.05; , P < 0.01; , P < 0.0001. D, BrdUrd and PI analysis of NB-19 cells by flow cytometry. The data are representative of two experiments showing similar results. Top, cells transfected with SCR; bottom, cells transfected with siRNA 1 þ 2. E, bar diagram of the distribution of cells in phases of cell cycle according to PI content from the experiments shown in D.
Assaymax Human Prealbumin Elisa Kit, supplied by Assaypro, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary anti ttr antibody  (ProSci Incorporated)
93
ProSci Incorporated primary anti ttr antibody
Figure 4. KD of Sulf-2 expression in MYCN-A neuroblastoma cells results in loss of viability. SK-N-BE(2) and NB-19 neuroblastoma cells were transfected with <t>siRNA</t> for Sulf-2 and examined for survival and proliferation. A, Western blot analysis of Sulf-2 expression on cell lysates obtained 48 hours after transfection. B, representative crystal violet stains of cells in culture 48 hours after transfection [top, SK-N-BE(2); bottom, NB-19]. C, cell viability was measured at indicated time. Data, mean (SD) fluorescence units (RLU) of quadruplicate samples and are representative of three separate experiments showing similar results; , P < 0.05; , P < 0.01; , P < 0.0001. D, BrdUrd and PI analysis of NB-19 cells by flow cytometry. The data are representative of two experiments showing similar results. Top, cells transfected with SCR; bottom, cells transfected with siRNA 1 þ 2. E, bar diagram of the distribution of cells in phases of cell cycle according to PI content from the experiments shown in D.
Primary Anti Ttr Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tthy  (Sino Biological)
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Sino Biological tthy
Figure 4. KD of Sulf-2 expression in MYCN-A neuroblastoma cells results in loss of viability. SK-N-BE(2) and NB-19 neuroblastoma cells were transfected with <t>siRNA</t> for Sulf-2 and examined for survival and proliferation. A, Western blot analysis of Sulf-2 expression on cell lysates obtained 48 hours after transfection. B, representative crystal violet stains of cells in culture 48 hours after transfection [top, SK-N-BE(2); bottom, NB-19]. C, cell viability was measured at indicated time. Data, mean (SD) fluorescence units (RLU) of quadruplicate samples and are representative of three separate experiments showing similar results; , P < 0.05; , P < 0.01; , P < 0.0001. D, BrdUrd and PI analysis of NB-19 cells by flow cytometry. The data are representative of two experiments showing similar results. Top, cells transfected with SCR; bottom, cells transfected with siRNA 1 þ 2. E, bar diagram of the distribution of cells in phases of cell cycle according to PI content from the experiments shown in D.
Tthy, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Reagents used in this research.

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: Reagents used in this research.

Article Snippet: , PA ELISA kit , EK1684 , Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Diagnostic Assay

Figure 4. KD of Sulf-2 expression in MYCN-A neuroblastoma cells results in loss of viability. SK-N-BE(2) and NB-19 neuroblastoma cells were transfected with siRNA for Sulf-2 and examined for survival and proliferation. A, Western blot analysis of Sulf-2 expression on cell lysates obtained 48 hours after transfection. B, representative crystal violet stains of cells in culture 48 hours after transfection [top, SK-N-BE(2); bottom, NB-19]. C, cell viability was measured at indicated time. Data, mean (SD) fluorescence units (RLU) of quadruplicate samples and are representative of three separate experiments showing similar results; , P < 0.05; , P < 0.01; , P < 0.0001. D, BrdUrd and PI analysis of NB-19 cells by flow cytometry. The data are representative of two experiments showing similar results. Top, cells transfected with SCR; bottom, cells transfected with siRNA 1 þ 2. E, bar diagram of the distribution of cells in phases of cell cycle according to PI content from the experiments shown in D.

Journal: Cancer Research

Article Title: MYCN-Dependent Expression of Sulfatase-2 Regulates Neuroblastoma Cell Survival

doi: 10.1158/0008-5472.can-13-2513

Figure Lengend Snippet: Figure 4. KD of Sulf-2 expression in MYCN-A neuroblastoma cells results in loss of viability. SK-N-BE(2) and NB-19 neuroblastoma cells were transfected with siRNA for Sulf-2 and examined for survival and proliferation. A, Western blot analysis of Sulf-2 expression on cell lysates obtained 48 hours after transfection. B, representative crystal violet stains of cells in culture 48 hours after transfection [top, SK-N-BE(2); bottom, NB-19]. C, cell viability was measured at indicated time. Data, mean (SD) fluorescence units (RLU) of quadruplicate samples and are representative of three separate experiments showing similar results; , P < 0.05; , P < 0.01; , P < 0.0001. D, BrdUrd and PI analysis of NB-19 cells by flow cytometry. The data are representative of two experiments showing similar results. Top, cells transfected with SCR; bottom, cells transfected with siRNA 1 þ 2. E, bar diagram of the distribution of cells in phases of cell cycle according to PI content from the experiments shown in D.

Article Snippet: Inserted sequences were subcloned into tet-inducible shRNA lentiviral vectors pLVCT-tTR-KRAB (Addgene; plasmid #11643) .

Techniques: Expressing, Transfection, Western Blot, Cytometry

Figure 5. Loss of Sulf-2 expression in MYCN-A neuroblastoma cells induces apoptosis. A, caspase 3/7 activity in Sulf-2 siRNA– transfected SK-N-BE(2) and NB-19 cells was measured using the ApoLive-Glo system. Data, mean (SD) luminescence units (RLU) of triplicate sample from one experiment and are representative of three independent experiments showing similar results; , P < 0.01; , P < 0.001. B, Western blot analysis of SK-N-BE(2) and NB-19 cells harvested 48 hours after transfection. C, apoptosis in NB-19 cells transfected as indicated in A was determined by flow cytometric analysis of Annexin V/PI staining; values, mean SEM of three independent experiments.

Journal: Cancer Research

Article Title: MYCN-Dependent Expression of Sulfatase-2 Regulates Neuroblastoma Cell Survival

doi: 10.1158/0008-5472.can-13-2513

Figure Lengend Snippet: Figure 5. Loss of Sulf-2 expression in MYCN-A neuroblastoma cells induces apoptosis. A, caspase 3/7 activity in Sulf-2 siRNA– transfected SK-N-BE(2) and NB-19 cells was measured using the ApoLive-Glo system. Data, mean (SD) luminescence units (RLU) of triplicate sample from one experiment and are representative of three independent experiments showing similar results; , P < 0.01; , P < 0.001. B, Western blot analysis of SK-N-BE(2) and NB-19 cells harvested 48 hours after transfection. C, apoptosis in NB-19 cells transfected as indicated in A was determined by flow cytometric analysis of Annexin V/PI staining; values, mean SEM of three independent experiments.

Article Snippet: Inserted sequences were subcloned into tet-inducible shRNA lentiviral vectors pLVCT-tTR-KRAB (Addgene; plasmid #11643) .

Techniques: Expressing, Activity Assay, Transfection, Western Blot, Staining