Journal: eLife

Article Title: An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis

doi: 10.7554/eLife.30126

Figure Lengend Snippet:

Article Snippet: antibody , Rat monoclonal anti-Jagged1 , DSHB (Iowa, USA) , Ts1.15h, RRID: AB_528317 , 1/300, IHC.

Techniques: Recombinant, Transfection, Protease Inhibitor, In Situ, Sequencing, Cloning, In Situ Hybridization, Construct, Generated, Plasmid Preparation, Mutagenesis, Software

Journal: JCI Insight

Article Title: Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome

doi: 10.1172/jci.insight.97497

Figure Lengend Snippet: (A) In nascent S-shaped body (SSB), Hnf4a is not detected. Representative image of n = 3. Scale bar: 25 μm. (B) Hnf4a is expressed in a subset of Jag1+ cells in the medial segment of the elongated SSB. Scale bar: 25 μm. Image is representative of n = 3. (C) Hnf4a is detected in presumptive proximal tubule (PT) cells and differentiated PT cells. Presumptive PT cells show weak Lotus tetragonolobus lectin (LTL) staining and are located more cortically, where early stages of nephrogenesis occur. Differentiated PT cells show strong LTL staining and are located closer to the medullary region. Image is representative of n = 3. Scale bar: 100 μm.

Article Snippet: We used the primary antibodies for GFP (1:500, Aves, GFP-1020), Jag1 (1:20, Developmental Studies Hybridoma Bank [DSHB], TS1.15H), Wt1 (1:100, Santa Cruz Biotechnology Inc., sc-7385), Biotin-LTL (1:500, Vector Laboratories, B-1325), FITC-LTL (1:200, Vector Laboratories, FL-1321), Slc12a1 (1:500, Proteintech, 18970-1-AP), Slc12a3 (1:300, MilliporeSigma, HPA028748), Hnf4a (1:1,000, Santa Cruz Biotechnology Inc., sc-8987X; or 1:500, Abcam, ab41898), Lrp2 (1:100, Santa Cruz Biotechnology Inc., sc-515772), and Ass1 (1:500, Proteintech, 16210-1-AP).

Techniques: Staining

Journal: Cancer Biology & Therapy

Article Title: Reprogramming of VEGF-mediated extracellular matrix changes through autocrine signaling

doi: 10.1080/15384047.2023.2184145

Figure Lengend Snippet: Immunoblot analysis of enzymes and receptors in cancer cells. (a) Changes in ADAMTS1, uPAR, LOX, MMP1 and MMP2 in 231_VEGF cells compared to 231_WT cells. (b) Changes in ADAMTS1, LOX, uPAR and MMP1 in wild type and VEGF overexpressing PC-3 and MCF-7 cells. (c) Changes in NRP-1 in MDA-MB-231, PC-3 and MCF-7 wild type and VEGF overexpressing cells.

Article Snippet: Antibodies cross-reactive with mouse/human ECM proteins and specific for human enzymes of interest included rabbit-polyclonal anti-Col1A1 antibody (1:1000; OriGene, Rockville, MD, USA), mouse monoclonal anti-FN1 antibody (1:2000 dilution; Proteintech, Rosemont, IL, USA), rabbit polyclonal anti-MMP-1 antibody (1:1000 dilution; Neo BioLab, Woburn, MA, USA), rabbit polyclonal anti-MMP2 (1:1000 dilution; GeneTex, Inc., Irvine, CA, USA), rabbit polyclonal anti-MMP-14 antibody (1:1000 dilution; Neo BioLab, Woburn, MA, USA), mouse monoclonal anti-lysyl oxidase (LOX) antibody (1:1000 dilution; GeneTex, Inc., Irvine, CA, USA), mouse monoclonal anti-ADAMTS1 antibody (1:500 dilution; OriGene, Rockville, MD, USA), rabbit polyclonal anti-uPAR (1:1000 dilution; GeneTex, Inc., Irvine, CA, USA), mouse monoclonal anti-α-SMA antibody (1:2000; Novus Biologicals, Littleton, CO, USA), rabbit monoclonal antibody against neuropilin-1 (NRP-1) (1:1000, clone D62C6, Cell Signaling, Danvers, MA, USA, rabbit polyclone anti-FLT1 (VEGFR1) antibody (1:1000, MyBioSource, San Diego, CA) and rabbit polyclonal anti-FAP-α antibody (1:1000, Ab207178, Abcam, Cambridge, UK).

Techniques: Western Blot