Journal: Nutrients

Article Title: Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism

doi: 10.3390/nu14061229

Figure Lengend Snippet: Comparison of IDO and TPH2 immuno-positive MOD, AD and H-score in different brain regions of rats in each group.

Article Snippet: The membranes were sealed with 5% non-fat milk in TBS-T solution and placed at room temperature for 4 h. The membranes were incubated overnight at 4 °C with the following primary antibodies: (1) rabbit anti-TPH1 antibody (1:1000, Boster, Wuhan, Hubei, China); (2) rabbit anti-IDO2 antibody (1:2000, Boster, Wuhan, Hubei, China); (3) rabbit anti-TPH2 antibody (1:1000, Boster, Wuhan, Hubei, China).

Techniques: Comparison

Journal: Nutrients

Article Title: Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism

doi: 10.3390/nu14061229

Figure Lengend Snippet: Expression of TPH2 in prefrontal cortex ( a – c ) and hippocampus ( d – f ) of three groups of rats. ( a , d ) CON group, ( b , e ) GH group, ( c , f ) GL group. DAB × 400.

Article Snippet: The membranes were sealed with 5% non-fat milk in TBS-T solution and placed at room temperature for 4 h. The membranes were incubated overnight at 4 °C with the following primary antibodies: (1) rabbit anti-TPH1 antibody (1:1000, Boster, Wuhan, Hubei, China); (2) rabbit anti-IDO2 antibody (1:2000, Boster, Wuhan, Hubei, China); (3) rabbit anti-TPH2 antibody (1:1000, Boster, Wuhan, Hubei, China).

Techniques: Expressing

Journal: Molecular Psychiatry

Article Title: SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behavior

doi: 10.1038/s41380-018-0260-9

Figure Lengend Snippet: Lack of SERT increases the number of functional PFC-to-DRN synapses. a rAAV-CAG-hChR2(H134R)-mCherry was bilaterally injected into the PFC of P4–P5 control or SERT-KO mice. Photograph showing mCherry expression after the PFC AAV injection (upper left). Optogenetic stimulation and electrophysiological patch clamp recordings were made starting at P28 in coronal sections containing the DRN, as shown by the photograph of the immunolabeling of PFC mCherry+ axons innervating to DRN 5-HT neurons, identified by the presence of the enzyme TPH2 (upper right). b Amplitude of optogenetically evoked EPSCs (oEPSCs) at synapses from PFC terminals onto DRN putative 5-HT neurons (left) and non-5-HT neurons (right) at various light stimulation intensities. In control (SERT Cre/+ ) (5-HT: n = 10 cells/5 animals; non-5-HT: n = 7 cells/4 animals); in SERT-KO (SERT Cre/Cre ) (5-HT: n = 10 cells/3 animals; non-5-HT: n = 6 cells/3 animals). Top: example traces at 9.8 mW (black/gray) and at 2 mW (red) stimulation); Bottom: input/output curves. Two-way ANOVA on 9.8 mW intensity: genotype x cell-type interaction (F 1,29 = 0.003, p = 0.95); Genotype main effect (F 1,29 = 9.32, * p < 0.01); Cell-type main effect (F 1,29 = 0.51, p = 0.48). c AMPAR/NMDAR ratios at synapses from PFC-to-DRN 5-HT neurons (left) and non-5-HT neurons (right) in control (5-HT: n = 10 cells/4 animals; non-5-HT: n = 7 cells/3 animals), and SERT-KO (5-HT: n = 11 cells/3 animals; non-5-HT: n = 6 cells; 3 animals). The AMPAR responses were calculated at the peak of −50 mV, whereas NMDAR responses were determined at + 40 mV, 50 ms after stimulation. Top: example traces; bottom: bar graphs. Two-ways ANOVA: Genotype x Cell-type interaction (F 1,30 = 0.007, p = 0.94); Genotype main effect (F 1,30 = 0.16, p = 0.69); Cell-type main effect (F 1,30 = 4.51, p < 0.05). Blue bars indicate blue light stimulation. Error bars represent SEM

Article Snippet: Subsequently, after a few hours of fixation in 4% PFA at 4 °C, brain slices were processed for immunohistochemistry using a rabbit polyclonal antibody against TPH2 (1:2000, Novus Biologicals, NB100-74555) to identify 5-HT neurons.

Techniques: Functional Assay, Injection, Control, Expressing, Patch Clamp, Immunolabeling