Journal: Cell reports

Article Title: NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation

doi: 10.1016/j.celrep.2021.108689

Figure Lengend Snippet:

Article Snippet: Membranes were transferred into TrueBlack WB blocking buffer (Biotium #23013) for 60 min at room temperature then were probed with primary antibodies diluted in TrueBlack WB antibody diluent (Biotium #23013) plus 0.2% Tween-20 for 18 hr at 4°C.

Techniques: Virus, Recombinant, Electron Microscopy, Transfection, Blocking Assay, Plasmid Preparation, CRISPR, Sequencing, Software

Journal: iScience

Article Title: Lipoxins reduce obesity-induced adipose tissue inflammation in 3D-cultured human adipocytes and explant cultures

doi: 10.1016/j.isci.2022.104602

Figure Lengend Snippet:

Article Snippet: Subsequently, sections were incubated with secondary antibodies (Alexa Fluor 488 Donkey Anti-Rabbit antibody, Alexa Fluor 594 Donkey Anti-Mouse antibody, Cy5 Donkey Anti-Goat antibody, Jackson Immuno Research) and DAPI (4′,6-Diamidino-2-Phenylindole, Dihydrochloride, Thermo Fisher) for 1 hour at room temperature, followed by three 5 min washes of 0.1% Triton X-100 in PBS and a 30 second incubation with TrueBlack (VWR) to quench lipofuscin autofluorescence.

Techniques: Recombinant, Reverse Transcription, Lysis, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Software