Journal: eLife

Article Title: Intrinsic mechanical sensitivity of mammalian auditory neurons as a contributor to sound-driven neural activity

doi: 10.7554/eLife.74948

Figure Lengend Snippet: Expression of transient response potential (TRP) channel in spiral ganglion neuron (SGN) soma and nerve terminals. The table shows a summary of immunofluorescent detection of TRP channels at SGN cell body (soma) and nerve terminals.

Article Snippet: The primary antibodies, chicken anti-Tuj1 (Abcam), mouse anti-myelin basic protein (Abcam), rabbit anti-Myo7a (Proteus Biosciences, Inc), mouse anti-Tuj1 (Abcam), rabbit anti-TRPA1 (Abcam), TRPC3 (Novus Biologicals), TRPC6 (Abcam), TRPV1 (Novus Biologicals), TRPV4 (Abcam), were incubated overnight at 4°C.

Techniques: Expressing

Journal: Anesthesiology

Article Title: Left Ventricular Hypertrophy Increases Susceptibility to Bupivacaine-induced Cardiotoxicity through Overexpression of Transient Receptor Potential Canonical Channels in Rats

doi: 10.1097/aln.0000000000003554

Figure Lengend Snippet: Fig. 6. Inhibitory effects of bupivacaine on sodium ion currents in transiently transfected human embryonic kidney (HEK)-293T cells with and without 1-oleoyl-2-acetyl-sn-glycerol, an activator of transient receptor potential canonical (TRPC) channels. (A, B) Representative sodium ion currents in the same cell perfused sequentially with standard solution (left), 3 μM bupivacaine (center), and 3 μM bupivacaine plus 100 μM 1-oleoyl-2-acetyl-sn-glycerol (right) in HEK-293T cells transfected with cardiac sodium channels (Nav1.5) alone (A), and HEK-293T cells transfected with Nav1.5 and TRPC3 (B). The voltage protocol is shown in the inset. (C) Sodium ion currents were reduced by 3 μM bupivacaine while there was no further reduction caused by 1-oleoyl-2-acetyl-sn-glycerol in HEK-293T cells transfected with Nav1.5 alone (n = 4 cells; 89.5 ± 3.2% and 89.1 ± 4.5% of control, respectively; P = 0.617). On the other hand, there were further reductions caused by 1-oleoyl-2-acetyl-sn-glycerol in HEK-293T cells cotransfected with Nav1.5 and TRPC3 (n = 4 cells; 85.9 ± 7.4% and 72.2 ± 6.5% of control, respectively; P = 0.048). There was a significant difference between Nav1.5 alone group and Nav1.5 with TRPC3 group in sodium ion current reduction caused by bupivacaine and 1-oleoyl-2-acetyl-sn-glycerol (n = 4 cells per group; P = 0.006). Data are mean ± SD. *P < 0.05 indicates significant differences between bupivacaine alone and coapplication of bupivacaine + 1-oleoyl- 2-acetyl-sn-glycerol. ‡P < 0.01 indicates a significant difference between HEK-293T cells transfected with Nav1.5 alone, and HEK-293T cells transfected with Nav1.5 and TRPC3. TRPC, transient receptor potential canonical

Article Snippet: The expression vectors carrying genes of the cardiac sodium ion channel (SCN5A) (NM_198056, gift from Dr. Hisao Yamamura, Nagoya City University, Japan),21 with and without human TrpC3-NMyc (Addgene #21085) were transiently transfected in HEK-293T cells using FuGENE HD (Promega Co., USA) in accordance with the manufacturer’s instructions.

Techniques: Transfection, Control