Journal: PLoS ONE

Article Title: Characterization of a long-term mouse primary liver 3D tissue model recapitulating innate-immune responses and drug-induced liver toxicity

doi: 10.1371/journal.pone.0235745

Figure Lengend Snippet: a Coculture spheroids were treated for 48 h with 5 μM and 25 μM Cyclosporine A (CsA) and stained with DAPI (blue) and for neutral lipids (green) using immunofluorescence. Scale bar = 50 μm b Quantitative analysis of neutral-lipid accumulation in CsA-treated spheroids. Data are represented as accumulation in % with respect to vehicle control (ctrl) ± standard deviation. (n = 8 spheroids per condition). c Coculture spheroids were treated with different concentrations of Cyclophosphamide (CPZ) and Troglitazone for 24 h and analyzed for BSEP gene expression via qRT-PCR. Data are expressed as x-fold change compared to vehicle control (ctrl) and corrected to GAPDH mRNA ± standard deviation (n = 4). Statistical significance is indicated * (p-value ≤ 0.05; Student’s t-test), ** (p-value ≤ 0.01; Student’s t-test), and *** (p-value ≤ 0.001; Student’s t-test), ns meant not significant (p-value > 0.05).

Article Snippet: On day 7 post seeding, coculture spheroids were treated with different concentrations of Chlorpromazine (CPZ, Sigma-Aldrich, Switzerland) and Troglitazone (LKT Labs, USA) for 24 h. Bile salt export pump (BSEP) downregulation was measured from pooled spheroids via qRT-PCR.

Techniques: Staining, Immunofluorescence, Control, Standard Deviation, Gene Expression, Quantitative RT-PCR