Journal: Journal of drug targeting

Article Title: Location of Stimuli-Responsive Peptide Sequences within Silk-Elastinlike Protein-Based Polymers Affects Nanostructure Assembly and Drug-Polymer Interactions

doi: 10.1080/1061186X.2020.1757099

Figure Lengend Snippet: Thermal circular dichroism peak reversibility and secondary structure properties of SELP nanogels.

Article Snippet: Circular dichroism spectra were obtained for SELP solutions (triplicate, 0.25 mg mL −1 in H 2 O) in 1 mm pathlength quartz cuvettes (Hellma Analytics, Müllheim, Baden Württemberg, Germany) using a J-1500 CD Spectrometer (Jasco Instruments, Easton, MD).

Techniques:

Journal:

Article Title: Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Saccharopolyspora erythraea Fermentation ▿

doi: 10.1128/AEM.02770-07

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: ZL1006 , eryK -triplicated and eryG -duplicated derivative of HL3168 E3, with the genotype PermK *- K + PermA *- G + PermE *- K-K-G , This work.

Techniques: Plasmid Preparation, Cloning, Conjugation Assay, Subcloning

Journal:

Article Title: Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Saccharopolyspora erythraea Fermentation ▿

doi: 10.1128/AEM.02770-07

Figure Lengend Snippet: HPLC analysis of Er production in fermentation of the controlled strain S. erythraea HL3168 E3, with standard Ers as controls.

Article Snippet: ZL1006 , eryK -triplicated and eryG -duplicated derivative of HL3168 E3, with the genotype PermK *- K + PermA *- G + PermE *- K-K-G , This work.

Techniques:

Journal:

Article Title: Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Saccharopolyspora erythraea Fermentation ▿

doi: 10.1128/AEM.02770-07

Figure Lengend Snippet: Genotypes and phenotypes of S. erythraea HL3168 E3 and its recombinant strains. (A) Restriction maps showing predicted fragment sizes upon SmaI and/or SphI digestion. (B) Southern analyses of genomic DNAs digested with SmaI or SphI by using the 1.7-kb eryK-containing fragment as a probe. (C) HPLC analyses of the Er production in fermentations. I, HL3168 E3; II, ZL1001; III, ZL1002; IV, ZL1003; V, ZL1004; VI, ZL1005; VII, ZL1006; VIII, ZL1007; IX, ZL1008; X, ZL1009. Solid circles, stars, and triangles indicate Er-C, Er-A, and Er-B, respectively. Each wavy line indicates the DNA fragment of the vector integrated by single-crossover homologous recombination.

Article Snippet: ZL1006 , eryK -triplicated and eryG -duplicated derivative of HL3168 E3, with the genotype PermK *- K + PermA *- G + PermE *- K-K-G , This work.

Techniques: Recombinant, Plasmid Preparation, Homologous Recombination

Journal:

Article Title: Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Saccharopolyspora erythraea Fermentation ▿

doi: 10.1128/AEM.02770-07

Figure Lengend Snippet: Titration of Er-A production in fermentations of S. erythraea HL3168 E3 and its recombinant strains, by assaying the antibacterial activity against B. pumilus. Error bars represent standard deviations.

Article Snippet: ZL1006 , eryK -triplicated and eryG -duplicated derivative of HL3168 E3, with the genotype PermK *- K + PermA *- G + PermE *- K-K-G , This work.

Techniques: Titration, Recombinant, Activity Assay

Journal:

Article Title: Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Saccharopolyspora erythraea Fermentation ▿

doi: 10.1128/AEM.02770-07

Figure Lengend Snippet: Productions of Ers, ratios of Er-A to Er-B plus Er-C, and ratios of transcript levels of eryK to eryG in S. erythraea HL3168 E3 and its recombinant strains

Article Snippet: ZL1006 , eryK -triplicated and eryG -duplicated derivative of HL3168 E3, with the genotype PermK *- K + PermA *- G + PermE *- K-K-G , This work.

Techniques: Recombinant

Journal:

Article Title: Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Saccharopolyspora erythraea Fermentation ▿

doi: 10.1128/AEM.02770-07

Figure Lengend Snippet: Transcript levels of eryK and eryG in S. erythraea HL3168 E3 and its recombinant strains. Error bars represent standard deviations. For each strain, the experiment has been independently carried out three times.

Article Snippet: ZL1006 , eryK -triplicated and eryG -duplicated derivative of HL3168 E3, with the genotype PermK *- K + PermA *- G + PermE *- K-K-G , This work.

Techniques: Recombinant