trif Search Results


trif  (Novus Biologicals)
94
Novus Biologicals trif
Ad-triggered, STING-dependent activation of TBK1 does not require S6Ks. (a) Immunoblot analysis of p-IRF3(S396), MyD88 and <t>Trif</t> in whole cell lysates of Myd88−/− Trif−/− BMDCs transduced with <t>Ad.</t> <t>β-actin</t> as loading control. (b) Immunoblot analysis of p-IRF3(S396) and MAVS in whole cell lysates of wild-type and Mavs−/− BMDCs transduced with Ad. β-actin as loading control. (c) Immunoblot analysis of p-TBK1(S172), TBK1, p-IRF3(S396), IRF3 and STING in whole cell lysates of wild-type and Tmem173−/− BMDCs transduced with Ad. β-actin as loading control. (d) Immunoblot analysis of p-IRF3(S396), IRF3 and TBK1 in whole cell lysates of wild-type and Tbk1−/− BMDCs transduced with Ad. β-actin as loading control. (e) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. (f) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), p-S6K1(S371), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. Data are representative of three independent experiments (a-f).
Trif, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pepinh trif tfa  (MedChemExpress)
93
MedChemExpress pepinh trif tfa
Ad-triggered, STING-dependent activation of TBK1 does not require S6Ks. (a) Immunoblot analysis of p-IRF3(S396), MyD88 and <t>Trif</t> in whole cell lysates of Myd88−/− Trif−/− BMDCs transduced with <t>Ad.</t> <t>β-actin</t> as loading control. (b) Immunoblot analysis of p-IRF3(S396) and MAVS in whole cell lysates of wild-type and Mavs−/− BMDCs transduced with Ad. β-actin as loading control. (c) Immunoblot analysis of p-TBK1(S172), TBK1, p-IRF3(S396), IRF3 and STING in whole cell lysates of wild-type and Tmem173−/− BMDCs transduced with Ad. β-actin as loading control. (d) Immunoblot analysis of p-IRF3(S396), IRF3 and TBK1 in whole cell lysates of wild-type and Tbk1−/− BMDCs transduced with Ad. β-actin as loading control. (e) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. (f) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), p-S6K1(S371), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. Data are representative of three independent experiments (a-f).
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anti trif  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc anti trif
Ad-triggered, STING-dependent activation of TBK1 does not require S6Ks. (a) Immunoblot analysis of p-IRF3(S396), MyD88 and <t>Trif</t> in whole cell lysates of Myd88−/− Trif−/− BMDCs transduced with <t>Ad.</t> <t>β-actin</t> as loading control. (b) Immunoblot analysis of p-IRF3(S396) and MAVS in whole cell lysates of wild-type and Mavs−/− BMDCs transduced with Ad. β-actin as loading control. (c) Immunoblot analysis of p-TBK1(S172), TBK1, p-IRF3(S396), IRF3 and STING in whole cell lysates of wild-type and Tmem173−/− BMDCs transduced with Ad. β-actin as loading control. (d) Immunoblot analysis of p-IRF3(S396), IRF3 and TBK1 in whole cell lysates of wild-type and Tbk1−/− BMDCs transduced with Ad. β-actin as loading control. (e) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. (f) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), p-S6K1(S371), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. Data are representative of three independent experiments (a-f).
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gene exp ticam1 mm00844508 s1  (Thermo Fisher)
85
Thermo Fisher gene exp ticam1 mm00844508 s1
mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes <t>Ticam1</t> (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.
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pef  (Addgene inc)
91
Addgene inc pef
mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes <t>Ticam1</t> (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.
Pef, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trif ticam1 proteintech  (Proteintech)
93
Proteintech trif ticam1 proteintech
mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes <t>Ticam1</t> (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.
Trif Ticam1 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trim69 detec tion  (Proteintech)
93
Proteintech trim69 detec tion
mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes <t>Ticam1</t> (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.
Trim69 Detec Tion, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti human icam 1 antibody  (R&D Systems)
90
R&D Systems goat anti human icam 1 antibody
mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes <t>Ticam1</t> (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.
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gene exp ticam1 hs01090712 m1  (Thermo Fisher)
86
Thermo Fisher gene exp ticam1 hs01090712 m1
mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes <t>Ticam1</t> (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.
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imgenex protein levels  (Novus Biologicals)
93
Novus Biologicals imgenex protein levels
mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes <t>Ticam1</t> (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.
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adaptor molecule tram  (Proteintech)
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Proteintech adaptor molecule tram
Primer sequences for PCR.
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Image Search Results


Ad-triggered, STING-dependent activation of TBK1 does not require S6Ks. (a) Immunoblot analysis of p-IRF3(S396), MyD88 and Trif in whole cell lysates of Myd88−/− Trif−/− BMDCs transduced with Ad. β-actin as loading control. (b) Immunoblot analysis of p-IRF3(S396) and MAVS in whole cell lysates of wild-type and Mavs−/− BMDCs transduced with Ad. β-actin as loading control. (c) Immunoblot analysis of p-TBK1(S172), TBK1, p-IRF3(S396), IRF3 and STING in whole cell lysates of wild-type and Tmem173−/− BMDCs transduced with Ad. β-actin as loading control. (d) Immunoblot analysis of p-IRF3(S396), IRF3 and TBK1 in whole cell lysates of wild-type and Tbk1−/− BMDCs transduced with Ad. β-actin as loading control. (e) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. (f) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), p-S6K1(S371), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. Data are representative of three independent experiments (a-f).

Journal: Nature immunology

Article Title: S6K-STING interaction regulates cytosolic DNA-mediated activation of the transcription factor IRF3

doi: 10.1038/ni.3433

Figure Lengend Snippet: Ad-triggered, STING-dependent activation of TBK1 does not require S6Ks. (a) Immunoblot analysis of p-IRF3(S396), MyD88 and Trif in whole cell lysates of Myd88−/− Trif−/− BMDCs transduced with Ad. β-actin as loading control. (b) Immunoblot analysis of p-IRF3(S396) and MAVS in whole cell lysates of wild-type and Mavs−/− BMDCs transduced with Ad. β-actin as loading control. (c) Immunoblot analysis of p-TBK1(S172), TBK1, p-IRF3(S396), IRF3 and STING in whole cell lysates of wild-type and Tmem173−/− BMDCs transduced with Ad. β-actin as loading control. (d) Immunoblot analysis of p-IRF3(S396), IRF3 and TBK1 in whole cell lysates of wild-type and Tbk1−/− BMDCs transduced with Ad. β-actin as loading control. (e) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. (f) Immunoblot analysis of p-TBK1(S172), p-IRF3(S396), p-S6K1(S371), S6K1 and S6K2 in whole cell lysates of wild-type and S6k1−/− S6k2−/− BMDCs transduced with Ad. β-actin as loading control. Data are representative of three independent experiments (a-f).

Article Snippet: The primary antibodies used for immunoblot analysis and IP were from the following sources: abcam: IFIT1 (ab11821); Cell Signaling Technology: phospho-TBK1 (#5483), TBK1 (3504), phospho-IRF3 (S396, #4947), IRF3 (#4302), STING (#3337), STING (#13647), p70S6K1 (#9202), phospho-p70S6K1 (S371, #9208), phospho-p70S6K1 (T389, #9234), phospho-S6 ribosomal protein (S240/244, #5364), S6K2 (#14130), MyD88 (#4283), IPS-1(#4983), ISG15 (#2743), cGAS antibody (#31659), HA-Tag antibody-Sepharose (#3956), HA-Tag antibody (#3724), HA-Tag antibody (#2367), Flag-Tag antibody-Sepharose (#5750), Flag-Tag antibody (#2368), Flag-Tag antibody (#8146), anti-rabbit IgG F(ab’)2 fragment Sepharose (#3400),, Myc-Tag antibody (#2278), GFP-Tag antibody (#2956); Abiocode: cGAS (R-3252-1); Santa Cruz Biotechnology: IRF3 (sc-9082 and sc-15991), TBK1 (sc-9910), histone H1 (sc-10806), p70S6Kβ (S6K2, sc-9381), rabbit anti-goat IgG-HRP (sc-2768), HA-probe (sc-7392), Myc-Tag antibody-Sepharose (sc-40 AC), luciferase antibody (sc-74548); Sigma: β-actin antibody (A5441); Millipore: p70S6K1 (05-781R); Novus Biologicals: Trif (NB-120-13810); and Zymed Laboratories: IRF3 (ZM3, 51-3200); Rockland Immunochemicals: anti-Flag antibody (#600-401-383).

Techniques: Activation Assay, Western Blot, Transduction

mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes Ticam1 (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.

Journal: International Journal of Molecular Sciences

Article Title: Transglutaminase 2 Facilitates Murine Wound Healing in a Strain-Dependent Manner

doi: 10.3390/ijms241411475

Figure Lengend Snippet: mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes Ticam1 (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.

Article Snippet: Quantitative PCR was performed in triplicate to examine the expression levels of Tgm1 (assay ID: Mm00498375_m1*), Tgm2 (Mm00436987_m1*), Tgm3 (Mm00436999_m1*), F13a1 (Mm00472334_m1*), Tgm4 (Mm00626039_m1*), Tgm5 (Mm00551325_m1*), Tgm6 (Mm00624922_m1*), Tgm7 (Mm03990491_m1*), Il6 (Mm00446190_m1*), Ifng (Mm00497611_m1*), Tnf (Mm00443258_m1*), Egf (Mm00438696_m1*), Fgf2 (Mm00433287_m1*), Tgfb2 (Mm00436955_m1*), Ticam1 (Mm00844508_s1*), Myd88 (Mm00440338_m1*), Nfkb1 (Mm00476361_m1*), Nfkbib (Mm00456849_m1*), Fndc4 (Mm00480765_m1*), Fndc3a (Mm01232694_m1*), Col1a1 (Mm00801666_g1*), Col1a2 (Mm00483888_m1*), Col3a1 (Mm01254476_m1*), Sdc4 (Mm00488527_m1*), with Hprt (Mm00446968_m1*) as the most suitable reference gene of four reference genes ( Hprt , GAPDH , 18S rRNA and β-2-microglubin ( B2M )) tested (expression unaffected by the experimental treatment).

Techniques: Generated, Isolation, Variant Assay, Gene Expression, Two Tailed Test

Primer sequences for PCR.

Journal: Frontiers in Pharmacology

Article Title: Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

doi: 10.3389/fphar.2021.753599

Figure Lengend Snippet: Primer sequences for PCR.

Article Snippet: Antibodies against Toll-like receptor 2 (TLR2), cluster of differentiation 14 (CD14), and TRIF-related adaptor molecule (TRAM) were purchased from Proteintech (IL, United States).

Techniques:

ABPS increased the expression of TLR4 and MyD88 in J774 A.1 cells. (A) The mRNA expression of TLR2, TLR4, TRAM, and MyD88. (B) The protein expression of TLR2, TLR4, TRAM, and MyD88. The blot shown is representative of one of the three similar experiments. Cells were treated 500 μg/ml ABPS for 24 h. NC: normal control; ABPS500: 500 μg/ml ABPS treated. * p < 0.05, ** p < 0.01 vs. NC. The values are presented as means ± SD.

Journal: Frontiers in Pharmacology

Article Title: Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

doi: 10.3389/fphar.2021.753599

Figure Lengend Snippet: ABPS increased the expression of TLR4 and MyD88 in J774 A.1 cells. (A) The mRNA expression of TLR2, TLR4, TRAM, and MyD88. (B) The protein expression of TLR2, TLR4, TRAM, and MyD88. The blot shown is representative of one of the three similar experiments. Cells were treated 500 μg/ml ABPS for 24 h. NC: normal control; ABPS500: 500 μg/ml ABPS treated. * p < 0.05, ** p < 0.01 vs. NC. The values are presented as means ± SD.

Article Snippet: Antibodies against Toll-like receptor 2 (TLR2), cluster of differentiation 14 (CD14), and TRIF-related adaptor molecule (TRAM) were purchased from Proteintech (IL, United States).

Techniques: Expressing