trbc1 Search Results


trbc1  (Caprico Biotechnologies)
90
Caprico Biotechnologies trbc1
The most common aberrancies of pan-T cell antigens in AITL. (A) PB specimen in which neoplastic CD4+ T cells had bright CD45 and PD-1 expression, and diminished CD4 expression, lack of CD3, CD7, and <t>TRBC1</t> with slightly larger side scatter. The same immunophenotyping was observed in BM, PB, and LN specimens. (B) FNA sample in which CD4+ aberrant T cells had bright CD4, CD2, and PD-1 expression, CD200 was positive, CD5, and CD45 were diminished; CD3, CD7, and TRBC1 were absent; Side scatter was enlarged.
Trbc1, supplied by Caprico Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tcrβ  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc tcrβ
Immunophenotype characterization of IELs in the intestinal mucosa control. Most IELs expressed <t>TCRβ</t> chains. Occasionally, CD56 + IELs were found, as well as TCRδ chain-positive IELs (i.e., TCRγδ + IELs). TCRB, TCR beta (β); TCRD, TCR delta (δ). TCR delta was analyzed using two different clones: H-41 (Santa Cruz, sc-100289) and E2E9T (Cell Signaling, TRDC/TCRδ (E2E9T) XP® <t>Rabbit</t> <t>mAb</t> #55750). Original magnification 200× and 400×.
Tcrβ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elife 2022  (Addgene inc)
93
Addgene inc elife 2022
Immunophenotype characterization of IELs in the intestinal mucosa control. Most IELs expressed <t>TCRβ</t> chains. Occasionally, CD56 + IELs were found, as well as TCRδ chain-positive IELs (i.e., TCRγδ + IELs). TCRB, TCR beta (β); TCRD, TCR delta (δ). TCR delta was analyzed using two different clones: H-41 (Santa Cruz, sc-100289) and E2E9T (Cell Signaling, TRDC/TCRδ (E2E9T) XP® <t>Rabbit</t> <t>mAb</t> #55750). Original magnification 200× and 400×.
Elife 2022, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elife 2022/product/Addgene inc
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elife 2022 - by Bioz Stars, 2026-05
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biotinylated anti tcrβ  (Novus Biologicals)
90
Novus Biologicals biotinylated anti tcrβ
Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional <t>biotinylated</t> anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.
Biotinylated Anti Tcrβ, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tcr β cell signaling technology  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc tcr β cell signaling technology
Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional <t>biotinylated</t> anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.
Tcr β Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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w iley o nline l ibrary  (Addgene inc)
92
Addgene inc w iley o nline l ibrary
Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional <t>biotinylated</t> anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.
W Iley O Nline L Ibrary, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trbc1 (jovi-1  (Ansell Healthcare)
90
Ansell Healthcare trbc1 (jovi-1
Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional <t>biotinylated</t> anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.
Trbc1 (Jovi 1, supplied by Ansell Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trbc1 (jovi-1 - by Bioz Stars, 2026-05
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anti-trbc1  (Ancell corporation)
90
Ancell corporation anti-trbc1
Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional <t>biotinylated</t> anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.
Anti Trbc1, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-trbc1/product/Ancell corporation
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trbc1 antibody jovi-1 murine igg22 monoclonal antibody clone  (GeneTex)
90
GeneTex trbc1 antibody jovi-1 murine igg22 monoclonal antibody clone
Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional <t>biotinylated</t> anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.
Trbc1 Antibody Jovi 1 Murine Igg22 Monoclonal Antibody Clone, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trbc1 + 2 mix pcr primer  (Kaneka Corp)
90
Kaneka Corp trbc1 + 2 mix pcr primer
Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional <t>biotinylated</t> anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.
Trbc1 + 2 Mix Pcr Primer, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-trbc1  (Becton Dickinson)
90
Becton Dickinson anti-trbc1
Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional <t>biotinylated</t> anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.
Anti Trbc1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The most common aberrancies of pan-T cell antigens in AITL. (A) PB specimen in which neoplastic CD4+ T cells had bright CD45 and PD-1 expression, and diminished CD4 expression, lack of CD3, CD7, and TRBC1 with slightly larger side scatter. The same immunophenotyping was observed in BM, PB, and LN specimens. (B) FNA sample in which CD4+ aberrant T cells had bright CD4, CD2, and PD-1 expression, CD200 was positive, CD5, and CD45 were diminished; CD3, CD7, and TRBC1 were absent; Side scatter was enlarged.

Journal: Frontiers in Medicine

Article Title: PD-1 combined with TRBC1 and pan-T cell antibodies for robustly monitoring angioimmunoblastic T-cell lymphoma

doi: 10.3389/fmed.2022.962428

Figure Lengend Snippet: The most common aberrancies of pan-T cell antigens in AITL. (A) PB specimen in which neoplastic CD4+ T cells had bright CD45 and PD-1 expression, and diminished CD4 expression, lack of CD3, CD7, and TRBC1 with slightly larger side scatter. The same immunophenotyping was observed in BM, PB, and LN specimens. (B) FNA sample in which CD4+ aberrant T cells had bright CD4, CD2, and PD-1 expression, CD200 was positive, CD5, and CD45 were diminished; CD3, CD7, and TRBC1 were absent; Side scatter was enlarged.

Article Snippet: PD-1 (EH12.2H7, BioLegend, APC), CD10, TRBC1 (JOVI.1, Caprico biotechnologies, FITC) and some pan-T cell antibodies (such as CD3, CD4, CD5, CD7, and CD8) were placed in an additional tube to confirm or exclude a supposed PTCL diagnosis and for patients with an equivocal clinical history or immunophenotyping.

Techniques: Expressing

Flow cytometric findings in non-T cell lymphoma. (A) CD4+ T cells with CD10 and bright PD-1 expression were observed in follicular lymphoma. (B) CD4+ T cells with CD200 and bright PD-1 expression were seen in tuberculous granuloma. (C) Expansion of CD4+T cells with bright PD-1 expression was present in nodular lymphocyte-predominant Hodgkin lymphoma. (D) CD4+ T cells with distinctive bright PD-1 expression were observed in reactive follicular hyperplasia. TRBC1 was polyclonal in these populations.

Journal: Frontiers in Medicine

Article Title: PD-1 combined with TRBC1 and pan-T cell antibodies for robustly monitoring angioimmunoblastic T-cell lymphoma

doi: 10.3389/fmed.2022.962428

Figure Lengend Snippet: Flow cytometric findings in non-T cell lymphoma. (A) CD4+ T cells with CD10 and bright PD-1 expression were observed in follicular lymphoma. (B) CD4+ T cells with CD200 and bright PD-1 expression were seen in tuberculous granuloma. (C) Expansion of CD4+T cells with bright PD-1 expression was present in nodular lymphocyte-predominant Hodgkin lymphoma. (D) CD4+ T cells with distinctive bright PD-1 expression were observed in reactive follicular hyperplasia. TRBC1 was polyclonal in these populations.

Article Snippet: PD-1 (EH12.2H7, BioLegend, APC), CD10, TRBC1 (JOVI.1, Caprico biotechnologies, FITC) and some pan-T cell antibodies (such as CD3, CD4, CD5, CD7, and CD8) were placed in an additional tube to confirm or exclude a supposed PTCL diagnosis and for patients with an equivocal clinical history or immunophenotyping.

Techniques: Expressing

Some AITL immunophenotyping could not be detected by the T-cell screen tube. (A,D,E,F) show BM specimens. (B,C) show tissue samples. (A) Aberrant T cells were CD3+, CD4+, and CD10+ with bright PD-1 expression, and CD7 was partially expressed. (B) Neoplastic T cells were CD4+, CD7+, and CD10+ with slightly diminished CD3 and CD5 expression and bright PD-1 expression. (C) Tumor cells were CD3+, CD5+, CD7+, and CD200+ with diminished CD4 expression and bright CD2, CD56, and PD-1 expression, and CD10 was negative (not shown). (D) Abnormal T cells were CD3+, CD4+, CD5+, and CD200+ with bright CD7 and PD-1 expression, and CD10 was negative. (E) An extremely low ratio of CD7 negative T cells was CD3+, CD4+, and CD10+ with bright CD5 and PD-1 expression. The immunophenotyping of neoplastic T cells shifted over time (E,F) before and after therapy, respectively. TRBC1 did not show polyclonal expression in these populations.

Journal: Frontiers in Medicine

Article Title: PD-1 combined with TRBC1 and pan-T cell antibodies for robustly monitoring angioimmunoblastic T-cell lymphoma

doi: 10.3389/fmed.2022.962428

Figure Lengend Snippet: Some AITL immunophenotyping could not be detected by the T-cell screen tube. (A,D,E,F) show BM specimens. (B,C) show tissue samples. (A) Aberrant T cells were CD3+, CD4+, and CD10+ with bright PD-1 expression, and CD7 was partially expressed. (B) Neoplastic T cells were CD4+, CD7+, and CD10+ with slightly diminished CD3 and CD5 expression and bright PD-1 expression. (C) Tumor cells were CD3+, CD5+, CD7+, and CD200+ with diminished CD4 expression and bright CD2, CD56, and PD-1 expression, and CD10 was negative (not shown). (D) Abnormal T cells were CD3+, CD4+, CD5+, and CD200+ with bright CD7 and PD-1 expression, and CD10 was negative. (E) An extremely low ratio of CD7 negative T cells was CD3+, CD4+, and CD10+ with bright CD5 and PD-1 expression. The immunophenotyping of neoplastic T cells shifted over time (E,F) before and after therapy, respectively. TRBC1 did not show polyclonal expression in these populations.

Article Snippet: PD-1 (EH12.2H7, BioLegend, APC), CD10, TRBC1 (JOVI.1, Caprico biotechnologies, FITC) and some pan-T cell antibodies (such as CD3, CD4, CD5, CD7, and CD8) were placed in an additional tube to confirm or exclude a supposed PTCL diagnosis and for patients with an equivocal clinical history or immunophenotyping.

Techniques: Expressing

Immunophenotype characterization of IELs in the intestinal mucosa control. Most IELs expressed TCRβ chains. Occasionally, CD56 + IELs were found, as well as TCRδ chain-positive IELs (i.e., TCRγδ + IELs). TCRB, TCR beta (β); TCRD, TCR delta (δ). TCR delta was analyzed using two different clones: H-41 (Santa Cruz, sc-100289) and E2E9T (Cell Signaling, TRDC/TCRδ (E2E9T) XP® Rabbit mAb #55750). Original magnification 200× and 400×.

Journal: Biomedicines

Article Title: Intraepithelial Lymphocytes and LAIR1 Expression in Celiac Disease

doi: 10.3390/biomedicines13102526

Figure Lengend Snippet: Immunophenotype characterization of IELs in the intestinal mucosa control. Most IELs expressed TCRβ chains. Occasionally, CD56 + IELs were found, as well as TCRδ chain-positive IELs (i.e., TCRγδ + IELs). TCRB, TCR beta (β); TCRD, TCR delta (δ). TCR delta was analyzed using two different clones: H-41 (Santa Cruz, sc-100289) and E2E9T (Cell Signaling, TRDC/TCRδ (E2E9T) XP® Rabbit mAb #55750). Original magnification 200× and 400×.

Article Snippet: The primary antibodies that were used were the following: CD3 (clone LN10, Leica Biosystems, Leica K.K., Shinjuku-ku, Tokyo, Japan), CD4 (4B12, Leica), CD8 (4B11, Leica), CD103 (EP206, Leica), granzyme B (11F1, Leica), TCRβ (TRBC1/TCRβ constant region 1 (E6Z3S) Rabbit mAb #79485, Cell Signaling Technology K.K., Chiyoda-ku, Tokyo, Japan), TCRδ (TRDC/TCRδ (E2E9T) XP ® Rabbit mAb #55750, Cell Signaling), CD56 (CD56-504-L-CE, Leica), CD16 (CD16-L-CE, Leica), LAIR1 (CD305, JAVI82A, created by Giovanna Roncador, Spanish National Cancer Research Center (CNIO, Centro Nacional de Investigaciones Oncologicas, calle Melchor Fernandez Almagro, 3, 28029 Madrid, Spain), PD-L1 (73-10, Leica), PD1 (CD279, NAT105, CNIO), BTLA (CD272, FLO67B, CNIO), TOX2 (TOM924D, CNIO), HVEM (TNFRSF14, ab47677, Abcam Tokyo, Ota city, Tokyo, Japan), CD163 (CD163-L-CE, Leica), HLA-DP-DQ (JS76, CNIO), IL4I1 (BALI265E,543H,573B, CNIO), and FOXP3 (236A, CNIO).

Techniques: Control, Clone Assay

Main phenotype of IELs in control intestinal mucosa. This figure summarizes the main immunophenotypes of IELs, including CD3+, CD8+, CD103+, LAIR+, and TCRβ+. An area with marked aggregation of IELs and immune cells in the lamina propria is shown. Original magnification 400×.

Journal: Biomedicines

Article Title: Intraepithelial Lymphocytes and LAIR1 Expression in Celiac Disease

doi: 10.3390/biomedicines13102526

Figure Lengend Snippet: Main phenotype of IELs in control intestinal mucosa. This figure summarizes the main immunophenotypes of IELs, including CD3+, CD8+, CD103+, LAIR+, and TCRβ+. An area with marked aggregation of IELs and immune cells in the lamina propria is shown. Original magnification 400×.

Article Snippet: The primary antibodies that were used were the following: CD3 (clone LN10, Leica Biosystems, Leica K.K., Shinjuku-ku, Tokyo, Japan), CD4 (4B12, Leica), CD8 (4B11, Leica), CD103 (EP206, Leica), granzyme B (11F1, Leica), TCRβ (TRBC1/TCRβ constant region 1 (E6Z3S) Rabbit mAb #79485, Cell Signaling Technology K.K., Chiyoda-ku, Tokyo, Japan), TCRδ (TRDC/TCRδ (E2E9T) XP ® Rabbit mAb #55750, Cell Signaling), CD56 (CD56-504-L-CE, Leica), CD16 (CD16-L-CE, Leica), LAIR1 (CD305, JAVI82A, created by Giovanna Roncador, Spanish National Cancer Research Center (CNIO, Centro Nacional de Investigaciones Oncologicas, calle Melchor Fernandez Almagro, 3, 28029 Madrid, Spain), PD-L1 (73-10, Leica), PD1 (CD279, NAT105, CNIO), BTLA (CD272, FLO67B, CNIO), TOX2 (TOM924D, CNIO), HVEM (TNFRSF14, ab47677, Abcam Tokyo, Ota city, Tokyo, Japan), CD163 (CD163-L-CE, Leica), HLA-DP-DQ (JS76, CNIO), IL4I1 (BALI265E,543H,573B, CNIO), and FOXP3 (236A, CNIO).

Techniques: Control

Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional biotinylated anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.

Journal: Cells

Article Title: Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane.

doi: 10.3390/cells8111388

Figure Lengend Snippet: Figure 4. Expression of Cdc42-Q61L selectively impairs internalization of the TCR-CD3 complex. (A) Schematic of the flow-cytometry based internalization assay; cells are labelled at 4 ◦C with a functional biotinylated anti-CD3ε and either (yellow) directly stained with Pacific Blue-streptavidin to measure surface expression of TCR-CD3 complex in resting cells, (blue) activated by incubation at 37 ◦C and stained with Pacific Blue-streptavidin to detect remaining TCR-CD3 at the cell surface after activation-induced internalization, or (red) activated by incubation at 37 ◦C, re-labelled with biotinylated anti-CD3ε and stained with Pacific Blue-streptavidin to detect total surface TCR-CD3 in activated cells. (B) Remaining TCR-CD3 at the cell surface detected by an antibody against CD3ε (clone OKT3) after activation-induced internalization in cells expressing an empty vector, GFP-WT-Cdc42, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (C) Surface expression of TCR-CD3 complex in cells expressing the same constructs as in (B). (D) Total surface TCR-CD3 in activated cells transfected as in (B). (E) Internalization of biotinylated Tf detected with Pacific Blue-streptavidin after incubation at 37 ◦C as described for anti-CD3ε in (A), in cells activated by soluble anti-CD3ε and expressing the same constructs as in (B). (F) Surface expression of Tf in cells expressing the same constructs as in (B). (G) Total Tf at the cell surface detected with biotinylated Tf and Pacific Blue-streptavidin in activated cells as in (F). Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), ** p < 0.01, n.s. not significant, unpaired, two-tailed student’s t-test.

Article Snippet: For non-activating conditions, cells were incubated for 30 min at 4 ◦C in serum-free RPMI with 1.5 μg/mL biotinylated anti-TCRβ (JOVI.1, NBP2-50355B, Novus Biologicals, Littleton, CO, USA).

Techniques: Expressing, Cytometry, Functional Assay, Staining, Incubation, Activation Assay, Plasmid Preparation, Construct, Transfection, Two Tailed Test