Journal: Oncology Letters

Article Title: Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing G 2 /M cell cycle arrest and apoptosis

doi: 10.3892/ol.2021.13134

Figure Lengend Snippet: Changes in TRAP1, P-ERK1/2/ERK1/2 and CDC25C protein expression levels in mouse forestomach carcinoma cells induced by luteolin (20 µM) and/or oxaliplatin (5 µM). (A-C) TRAP1, P-ERK1/2/ERK1/2, CDC25C and β-actin protein expression levels were assessed by western blot analysis, and quantitative analysis of protein expression levels is shown in the histogram. *P<0.05, **P<0.01. Experiments were repeated at least in triplicate. Lut, luteolin; Oxa, oxaliplatin; TRAP1, tumor necrosis factor receptor-associated protein 1; ERK1/2, extracellular-regulated protein kinases1/2; CDC25C, cell division cycle 25 homolog C.

Article Snippet: The primary antibodies used were: β-actin mouse monoclonal antibody (cat. no. TA-09; 1:2,000; OriGene Technologies, Inc.), Bcl-2 rabbit polyclonal antibody (cat. no. ab196495, 1:1,000; Abcam), BCL-2-associated X protein (Bax) rabbit monoclonal antibody (cat. no. ab182734; 1:1,000; Abcam), cyclin A2 rabbit monoclonal antibody (cat. no. ab181591; 1:2,000; Abcam), cyclin B1 rabbit monoclonal antibody (cat. no. ab32053; 1:1,000; Abcam), cyclin-dependent kinase-1 (CDK1) rabbit monoclonal antibody (cat. no. ab133327; 1:20,000; Abcam), tumor necrosis factor receptor-associated protein 1 (TRAP1) rabbit polyclonal antibody (cat. no. 10325-1-AP; 1:2,000; ProteinTech Group, Inc.), cell division cycle 25 homolog C (CDC25C) mouse monoclonal antibody (cat. no. 66912-1-lg; 1:2,000; ProteinTech Group, Inc.), extracellular-regulated protein kinases1/2 (ERK1/2) rabbit polyclonal antibody (cat. no. 9102s; 1:1,000; Cell Signaling Technology, Inc.) and p-ERK1/2 mouse monoclonal antibody (cat. no. 9106s; 1:1,000; Cell Signaling Technology, Inc.).

Techniques: Expressing, Western Blot

Journal: eLife

Article Title: Intrinsic OXPHOS limitations underlie cellular bioenergetics in leukemia

doi: 10.7554/eLife.63104

Figure Lengend Snippet:

Article Snippet: Transfected construct ( Homo sapien ) , shRNA to TRAP1 , Origene , CAT#: TL300868V , shRNA lentiviral particles packaged from pGFP-C-shLenti vector.

Techniques: Isolation, Lysis, Transfection, Construct, shRNA, Plasmid Preparation, Sequencing, Recombinant, Software

Journal: Environmental Science and Pollution Research International

Article Title: Gene expression signatures in PCB-exposed Slovak children in relation to their environmental exposures and socio-physical characteristics

doi: 10.1007/s11356-022-20018-2

Figure Lengend Snippet: List of selected 11 gene name, function, and percentage of population with transcriptional changes (both up ( +) and down ( −) regulated)

Article Snippet: 11 , TRAP1-Hs00212476_m1 , TNF receptor–associated protein 1; diseases associated with TRAP1 include Vacterl association and hereditary multiple exostoses , − 0.63 ( n = 52, 94.54%) + 0.10 ( n = 3, 5.45%).

Techniques: Expressing, Transformation Assay, Amplification

Journal: Virologica Sinica

Article Title: Hsp90 β is critical for the infection of severe fever with thrombocytopenia syndrome virus.

doi: 10.1016/j.virs.2023.11.008

Figure Lengend Snippet: Fig. 5. Hsp90 β interacts with NSs of SFTSV. A Co-immunoprecipitation (Co-IP) results of NSs with endogenous Hsp90 isomers. HEK293T cells were transfected with plasmids expressing HA-NP or HA-NSs. After 48 h, cells were lysed for Co-IP analysis with hemagglutinin (HA) antibodies or mouse IgG (control). The immuno- precipitated or input proteins were analyzed using Western blotting with anti-HA, anti-Hsp90 α1, anti-Hsp90 β, anti-GRP94, or anti-TRAP1 antibodies. The protein bands are indicated. (B, C) NSs interact with exogenous Hsp90 β. HEK293T cells were transfected or co-transfected with plasmids expressing HA-NSs, Flag-HSP90α, and Flag-HSP90β as indicated. After 48 h, cells were lysed for Co-IP analysis with anti-HA or anti-Flag antibodies. The input and immunoprecipitated samples were immunoblotted with indicated antibodies. (D, E) Hsp90 β colocalized with NSs. D HEK293 cells transfected with plasmids expressing Flag-Hsp90 α (upper panel) or Flag-Hsp90 β (lower panel) were super-infected with SFTSV at 24 h post-transfection. E HEK293 cells transfected with plasmids expressing Flag-Hsp90 β with or without HA-NSs. At 24 h post-infection/transfection, cells were immunostained with anti-Flag (mouse-derived) and anti-NSs (rabbit-derived) antibodies, followed by staining with AF488-conjugated anti-mouse IgG and AF594-conjugated anti-rabbit IgG, respectively. Cell nuclei were stained with DAPI. The small image in the upper right corner is the enlarged image of box region in (D) and (E). Scale bars, 20 μm (D) or 50 μm (E).

Article Snippet: Mouse anti-β-actin (66009-1-Ig), anti-Hsp90 α (60318-1-Ig), anti-Hsp90 β (67450-1-Ig), anti-GRP94 (60012-2-Ig), and anti-TRAP1 antibodies (67693-1-Ig) used in this study were purchased from ProteinTech (Wuhan, China).

Techniques: Immunoprecipitation, Co-Immunoprecipitation Assay, Transfection, Expressing, Control, Western Blot, Infection, Derivative Assay, Staining

Journal: BMC Medical Genomics

Article Title: Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood

doi: 10.1186/1755-8794-2-49

Figure Lengend Snippet: The panel of candidate reference genes chosen for validation by qRT-PCR.

Article Snippet: TRAP1 , Hs00972326_m1 , NM_016292 , heat shock protein 75.

Techniques: Biomarker Discovery

Journal: BMC Medical Genomics

Article Title: Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood

doi: 10.1186/1755-8794-2-49

Figure Lengend Snippet: Gene Ontology (GO) Annotation of genes analyzed in GeNorm

Article Snippet: TRAP1 , Hs00972326_m1 , NM_016292 , heat shock protein 75.

Techniques: Binding Assay, Activity Assay, Protein Binding, Membrane, Clinical Proteomics

Journal: BMC Medical Genomics

Article Title: Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood

doi: 10.1186/1755-8794-2-49

Figure Lengend Snippet: Identification of the most stably expressed reference genes using GeNorm analysis . RNA samples from whole-blood from 20 MS patients and 20 matched controls were analyzed using qRT-PCR. A. Average expression stability values (M) of the reference genes during step-wise exclusion of the least stable control gene. The x- axis shows the gene with the highest M value (the lowest stability) for this set of genes. The least stable gene was excluded after each iteration. B. Determination ofthe optimal number of reference genes for normalization. V is the pair-wise variation of two sequential normalization factors. The least number of genes for each V<0.15 was selected as the optimal set of genes for normalization . In this study, the four most stably expressed genes (TRAP1, FPGS, DECR1 and PPIB) represent the optimal set of genes for normalization.

Article Snippet: TRAP1 , Hs00972326_m1 , NM_016292 , heat shock protein 75.

Techniques: Stable Transfection, Quantitative RT-PCR, Expressing, Control

Journal: BMC Medical Genomics

Article Title: Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood

doi: 10.1186/1755-8794-2-49

Figure Lengend Snippet: Diagnosis effect on the candidate control genes.

Article Snippet: TRAP1 , Hs00972326_m1 , NM_016292 , heat shock protein 75.

Techniques: Biomarker Discovery, Control

Journal: BMC Medical Genomics

Article Title: Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood

doi: 10.1186/1755-8794-2-49

Figure Lengend Snippet: RNA expression values are shown for the four most stably expressed genes (TRAP1, FPGS, DECR1 and PPIB) for multiple sclerosis (MS) and controls subjects . Circles represent individual qRT-PCR Ct average raw values for MS samples (blue, n = 20) and age and gender matched controls (red, n = 20). Boxes represent the 25 th and 75 th quartile and lines represent the median. Whiskers represent the range of data for the 10 th and 90 th quartile.

Article Snippet: TRAP1 , Hs00972326_m1 , NM_016292 , heat shock protein 75.

Techniques: RNA Expression, Stable Transfection, Quantitative RT-PCR