transwell inserts Search Results


filter pore size transwell insert  (Greiner Bio)
96
Greiner Bio filter pore size transwell insert
Filter Pore Size Transwell Insert, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/filter pore size transwell insert/product/Greiner Bio
Average 96 stars, based on 1 article reviews
filter pore size transwell insert - by Bioz Stars, 2026-03
96/100 stars
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matrigel coated 24 well plate cell culture inserts  (Greiner Bio)
96
Greiner Bio matrigel coated 24 well plate cell culture inserts
Matrigel Coated 24 Well Plate Cell Culture Inserts, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matrigel coated 24 well plate cell culture inserts/product/Greiner Bio
Average 96 stars, based on 1 article reviews
matrigel coated 24 well plate cell culture inserts - by Bioz Stars, 2026-03
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pore size pet thincert transwell inserts  (Greiner Bio)
96
Greiner Bio pore size pet thincert transwell inserts
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Pore Size Pet Thincert Transwell Inserts, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pore size pet thincert transwell inserts/product/Greiner Bio
Average 96 stars, based on 1 article reviews
pore size pet thincert transwell inserts - by Bioz Stars, 2026-03
96/100 stars
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transwell insert costar  (Corning Life Sciences)
90
Corning Life Sciences transwell insert costar
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Transwell Insert Costar, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transwell insert costar/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
transwell insert costar - by Bioz Stars, 2026-03
90/100 stars
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8-μm pore corning 24-well transwell polycarbonate inserts  (Corning Life Sciences)
90
Corning Life Sciences 8-μm pore corning 24-well transwell polycarbonate inserts
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
8 μm Pore Corning 24 Well Transwell Polycarbonate Inserts, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/8-μm pore corning 24-well transwell polycarbonate inserts/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
8-μm pore corning 24-well transwell polycarbonate inserts - by Bioz Stars, 2026-03
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insert corning transwell 3414  (Corning Life Sciences)
90
Corning Life Sciences insert corning transwell 3414
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Insert Corning Transwell 3414, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/insert corning transwell 3414/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
insert corning transwell 3414 - by Bioz Stars, 2026-03
90/100 stars
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fluoroblok transwell inserts for 24-well plates with 3-mm pores  (Corning Life Sciences)
90
Corning Life Sciences fluoroblok transwell inserts for 24-well plates with 3-mm pores
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Fluoroblok Transwell Inserts For 24 Well Plates With 3 Mm Pores, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluoroblok transwell inserts for 24-well plates with 3-mm pores/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
fluoroblok transwell inserts for 24-well plates with 3-mm pores - by Bioz Stars, 2026-03
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24-mm-diameter transwell permeable inserts with pores  (Corning Life Sciences)
90
Corning Life Sciences 24-mm-diameter transwell permeable inserts with pores
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
24 Mm Diameter Transwell Permeable Inserts With Pores, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/24-mm-diameter transwell permeable inserts with pores/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
24-mm-diameter transwell permeable inserts with pores - by Bioz Stars, 2026-03
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inserts 3422  (Corning Life Sciences)
90
Corning Life Sciences inserts 3422
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Inserts 3422, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inserts 3422/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
inserts 3422 - by Bioz Stars, 2026-03
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24-well permeable 8mm pore pet transwell inserts with matrigel coating corning 354480  (Corning Life Sciences)
90
Corning Life Sciences 24-well permeable 8mm pore pet transwell inserts with matrigel coating corning 354480
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
24 Well Permeable 8mm Pore Pet Transwell Inserts With Matrigel Coating Corning 354480, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/24-well permeable 8mm pore pet transwell inserts with matrigel coating corning 354480/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
24-well permeable 8mm pore pet transwell inserts with matrigel coating corning 354480 - by Bioz Stars, 2026-03
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basement membrane matrix-coated upper sides of transwell inserts corning  (Corning Life Sciences)
90
Corning Life Sciences basement membrane matrix-coated upper sides of transwell inserts corning
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Basement Membrane Matrix Coated Upper Sides Of Transwell Inserts Corning, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/basement membrane matrix-coated upper sides of transwell inserts corning/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
basement membrane matrix-coated upper sides of transwell inserts corning - by Bioz Stars, 2026-03
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polytetrafluoroethylene filters transwell transwell® with pore polyester membrane insert, sterile  (Corning Life Sciences)
90
Corning Life Sciences polytetrafluoroethylene filters transwell transwell® with pore polyester membrane insert, sterile
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Polytetrafluoroethylene Filters Transwell Transwell® With Pore Polyester Membrane Insert, Sterile, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one transwell insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.

Journal: Gut Microbes

Article Title: SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella -containing vacuoles in vivo

doi: 10.1080/19490976.2021.1973836

Figure Lengend Snippet: Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one transwell insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.

Article Snippet: Cells were seeded on 3 µm pore size PET ThinCert transwell inserts (Greiner Bio-one, Kremsmünster, Austria) and cultured for 10–12 days with medium change every other day to obtain a covalent layer of polarized epithelial cells.

Techniques: In Vivo, In Vitro, Mutagenesis, Sequencing, Infection, Generated, Blocking Assay