Journal: Bioconjugate Chemistry

Article Title: Enhancing Reactivity for Bioorthogonal Pretargeting by Unmasking Antibody-Conjugated trans-Cyclooctenes

doi: 10.1021/bc500605g

Figure Lengend Snippet: Figure 5. Translation of dual orthogonal coupling to different antibodies. (A) Anti-TfR and EpCAM antibodies had low TCO reactivity (12−15%) after direct amine-conjugation, but the PEG24− TCO increased reactive TCO density 3- to 4-fold. *MALDI-TOF analysis of the TCO−PEG24-modified anti-EpCAM antibody was limited by difficulties with desorption. (B) Flow cytometry results for TCO- and TCO−PEG24-modified anti-EpCAM and TfR antibodies on A431 cells after reaction with 10 nM tetrazine−QDs for 30 min, showing 5-fold signal enhancements for the PEG24−TCO. (C) Confocal images of A431 cells labeled with (i, iv) anti-EpCAM, (ii, v) anti-TfR, or (iii, vi) nonbinding control antibodies. The antibodies were modified with (i−iii) TCO or (iv−vi) PEG24−TCO at the highest densities and reacted with tetrazine−QD at 10 nM for 30 min. Scale bar: 20 μm. Error bars represent the standard error of at least three independent experiments.

Article Snippet: Monoclonal mouse antibodies specific for human EpCAM (IgG2b, clone 158206) and transferrin receptor (TfR, IgG1, clone 29806) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Conjugation Assay, Flow Cytometry, Labeling, Control

Journal:

Article Title: Phospholipase D2 Is Localized to the Rims of the Golgi Apparatus in Mammalian Cells

doi: 10.1091/mbc.02-04-0059

Figure Lengend Snippet: PLD2 localization with different organelles in rat NRK cells. Cells were prepared for immunofluorescence microscopy and incubated with rabbit PLD2 antibody PLD2-27 (A, D, G, and J) and costained with monoclonal antibodies to the ER marker BiP (B); the transferrin receptor (E), a marker of early endosomes and the plasma membrane; and lgp120 (H), a late endosome/lysosomal marker. (K) Cells were stained with caveolin-1, a marker for caveoli; the arrow corresponds to caveolin-1 localized to the plasma membrane. Images were merged to determine overlap between PLD2 (red) and the respective marker proteins (green; C, F, I, and L). Areas of maximal overlap are yellow. (L) Inset, enlargement of the region of overlap between PLD2 and caveolin-1 in the perinuclear Golgi apparatus. The arrow indicates areas where PLD2 puncta (red) either overlap completely with caveolin-1 (green) or are directly adjacent. Images are from projected Z-series. Bars, 10 μm.

Article Snippet: Purified monoclonal anti-rat transferrin receptor antibodies were purchased from Cedarlane Laboratories (Hornby, Ontario, Canada).

Techniques: Immunofluorescence, Microscopy, Incubation, Marker, Staining

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: Reagents used in this research.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Diagnostic Assay

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The cross-reaction rates of 9 protein markers with each detection antibody (%).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The cross-reaction rates of 9 protein markers with each capture antibody (%).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The LLDs and BDLs of liquid protein chip for 9 protein markers (pg/mL).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The S -curves of 9 protein markers in the same one detection by using liquid protein chip. ( A ): SF S -curve; ( B ): sTfR S -curve; ( C ): CRP S -curve; ( D ): RBP4 S -curve; ( E ): ApoB S -curve; ( F ): AFP S -curve; ( G ): PA S -curve; ( H ): CEA S -curve; ( I ): D-D S -curve.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The regression equations and determination coefficients of 9 protein markers detected by the liquid protein chip.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Marker

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The recovery rates of 9 protein markers detected by liquid protein chip simultaneously ( n = 3).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The within-run precisions of 9 protein markers by liquid protein chip ( n = 10).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Chromatin Immunoprecipitation

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The between-run precisions of 9 protein markers by liquid protein chip ( D = 6, n = 3).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Chromatin Immunoprecipitation

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: Correlation analysis between liquid protein chip method and other methods for detecting the 9 protein markers of the same 16 serum samples. ( A ): The correlation analysis of SF; ( B ): The correlation analysis of sTfR; ( C ): The correlation analysis of CRP; ( D ): The correlation analysis of RBP4; ( E ): The correlation analysis of ApoB; ( F ): The correlation analysis of AFP; ( G ): The correlation analysis of PA; ( H ): The correlation analysis of CEA; ( I ): The correlation analysis of D-D.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The correlation analysis between the liquid protein chip method and other methods for the detection of 9 protein markers in the same 16 serums ( n = 16).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The paired comparison between liquid protein chip method and other methods for the detection of 9 protein markers of serums ( n = 16).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The analytical specificities of different interferences for the liquid protein chip in detecting 9 protein markers (%).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The comparison of the LLDs of 9 protein markers between liquid protein chip and other methods.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay