Journal: Stem Cell Reviews and Reports

Article Title: The Treasury of Wharton's Jelly

doi: 10.1007/s12015-021-10217-8

Figure Lengend Snippet: Flow cytometry. A typical example of FACS based expression analysis for transcription factors (upper histograms) and surface receptors (lower histograms) of VSELs. Each histogram compares given antibodies (given in black numbers) and specifc isotype controls (given in red numbers). Percentage of positive VSELs and the median fluorescence were assessed

Article Snippet: For fixation, permeabilization and staining procedures buffers by “Transcription Factor Staining Buffer Set” by Miltenyi Biotec (Berglisch Gladbach, Germany) were used.

Techniques: Flow Cytometry, Expressing, Fluorescence

Journal: Stem Cell Reviews and Reports

Article Title: The Treasury of Wharton's Jelly

doi: 10.1007/s12015-021-10217-8

Figure Lengend Snippet: Expression of pluripotency associated marker proteins examined by Immunofluorescence staining. VSELs were separated from UC-MSCs mass population, the tiny cells are between 5 and 7 μm and surrounded with dark spots ( a ). VSEL suspension cells were stained extracellular with anti-bodies against SSEA-4, CD184, and CD133 followed by nuclear staining of transcription factors Nanog, Oct-4, and Sox-2. Expression of each marker was detected. The nuclei were stained with HOECHST. Weak background Fluorescence signals of isotype controls were subtracted. The scale bars represented 50 μm ( b ). The magnification for SSEA-4/Sox-2 and CD133/Oct-4 was 20-fold and for CD184/Nanog 10-fold

Article Snippet: For fixation, permeabilization and staining procedures buffers by “Transcription Factor Staining Buffer Set” by Miltenyi Biotec (Berglisch Gladbach, Germany) were used.

Techniques: Expressing, Marker, Immunofluorescence, Staining, Suspension, Fluorescence