Journal: PLoS Genetics

Article Title: Innate immune signaling in Drosophila shifts anabolic lipid metabolism from triglyceride storage to phospholipid synthesis to support immune function

doi: 10.1371/journal.pgen.1009192

Figure Lengend Snippet: ( A ) Schematic representation of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) synthesis via the Kennedy pathway. ( B ) Late third instar fat body levels of eas , Pect , and CG7149 transcripts, normalized to Rp49 , n = 7/group. ***p = 0.0002 and ****p = 0.0001 versus GFP. ( C ) Late third instar fat body levels of CG2201 , Pcyt1 , and bbc transcripts, normalized to Rp49 . n = 7/group. **p ≤ 0.0071 versus GFP. ( D,E ) Western blot analysis of eas (D) and Pcyt1 (E) protein levels in fat bodies expressing GFP or Toll 10b under control of r4-GAL4. Histone H3 levels are shown as loading controls. ( F,G ) eas (F) and Pcyt1 (G) mRNA levels, normalized to Rp49 , in third instar larval fat bodies from uninfected controls, larvae subjected to sterile injury, and larvae infected with Enterococcus faecalis at 6–36 hours post infection, n = 7-10/group. *p ≤ 0.0496, **p ≤ 0.0035, and ***p = 0.0009 versus uninfected controls. ( H ) Mass spectrometry analysis of total phosphatidylethanolamine levels (PE, left) and phosphatidylcholine levels (PC, right) in lipid extracts from late third instar larval fat bodies expressing GFP or Toll 10b , n = 6/group. *p = 0.0417 and **p = 0.0080 versus GFP. ( I ) Mass spectrometry analysis of PE (left) and PC species (right) in lipid extracts from late third instar larval fat bodies expressing GFP or Toll 10b , n = 6/group. *p < 0.05, **p < 0.01 versus GFP. Data are shown as means ± SD. p values were determined by Student’s unpaired t test (B, C, H, I) and one-way ANOVA with Dunnett’s multiple comparisons test (F, G).

Article Snippet: Standards were phosphatidylcholine (3 μL at 1 μg/μL; egg L-α-phosphatidylcholine Avanti Polar Lipids, 131601) and phosphatidylethanolamine (3 μL at 1 μg/μL; egg L-α-phosphatidylethanolamine Avanti Polar Lipids, 840021).

Techniques: Western Blot, Expressing, Control, Sterility, Infection, Mass Spectrometry