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Image Search Results
Journal: Nature Communications
Article Title: Autologous HIV-specific T cell therapy targeting conserved epitopes is well-tolerated in six adults with HIV: an open-label, single-arm phase 1 study
doi: 10.1038/s41467-025-59810-2
Figure Lengend Snippet: a Two to four T cell clones were present in both the HST-NEET product and peripheral blood TCR repertoires for n = 4 participants. Reported TCRs were sequenced from PBMC samples pulsed with HIV peptides shown in parentheses above respective graphs. b RESIST-006 HST-NEETs and sequenced follow-up PBMC samples did not share any T cell clones. All Gag/Pol Mos1/2 and Nef reactive clones present in RESIST-006 HST-NEETs are shown. c Tracking of T cell clones present in two or more sequenced RESIST-006 follow-up PBMC samples. d T cell clones revealed in three of the highest IFN-γ producing RESIST-003 follow-up PBMCs at weeks 63, 90, and 103. These T cell clones were not present in the RESIST-003 HST-NEETs but had high productive frequencies coinciding with high IFN-γ T cell activation upon stimulation with Gag/Pol Mos1 and Mos2 peptides. Vertical dashed lines (--) indicate infusion one and infusion two, respectively. Source data are provided as a Source Data file.
Article Snippet: Antibodies used in panels for cell surface staining differed between the GMP and non-GMP laboratories, and were combinations of the following: CD3 PE-Cy7 (Cat: 317334, Biolegend), CD3 PerCP-Vio700 (Cat: 130-113-141, Miltenyi), CD3 BV785 (Cat: 317330, Biolegend), CD4 BV605 (Cat: 317438, Biolegend), CD4 PE Vio770 (Cat: 130-113-227, Miltenyi), CD8a BV421 (Cat: 301036, Biolegend), CD8a APC Vio770 (Cat: 130-110-681, Miltenyi), CCR7 Alexa Fluor 700 (Cat: 353244, Biolegend), CCR7 FITC (Cat: 130-120-468, Miltenyi), CD45RO PE-Dazzle 594 (Cat: 304248, Biolegend), CD45RO PE (Cat: 130-113-559, Miltenyi), CD19 FITC (Cat: 130-113-645, Miltenyi), CD14 VioBlue (Cat: 130-110-524, Miltenyi), CD16 PE (Cat: 130-113-393, Miltenyi), CD56 BV650 (Cat: 318344, Biolegend), CD56 PE (Cat: 130-113-312, Miltenyi), TCRγδ APC-Fire 750 (Cat: 331228, Biolegend), TCRγδ (Cat: 130-113-512, Miltenyi), TCRαβ PerCP/Cyanine5.5 (Cat: 306724, Biolegend),
Techniques: Clone Assay, Activation Assay