OriGene usp19 human shrna plasmid kit

Univariate Cox Model for the Association Between Survival and Clinicopathological Features in GC

Usp19 Human Shrna Plasmid Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tr308463/USP19+Human+shRNA+Plasmid+Kit/pmc07457554-149-15-28
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usp19 human shrna plasmid kit - by Bioz Stars, 2026-10

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Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: Univariate Cox Model for the Association Between Survival and Clinicopathological Features in GC

Article Snippet: The specific kit for knockdown of USP19 was ordered from the commercial company (OriGene, Ltd.; USP19 Human shRNA Plasmid Kit [Locus ID 10,869], CAT#: TR308463, ordered from https://www. origene.com/catalog/rnai/shrna-plasmids/tr308463/usp19-human-shrna- plasmid-kit- locus- id-10,869 ).

Techniques: Expressing

Relative expression of USP19 in GC cell lines and GES1. ( A ) Differential expression of USP19 mRNA in gastric cell lines by real-time PCR assay. ( B ) The differential expression of USP19 protein in gastric cell lines by WB analysis. ( C ) Differential expression and distribution of USP19 protein in gastric cell lines by confocal analysis, Scale bar=20 μm. ( D ) Relative expression of USP19 in paired 212 GC samples and ANTs by IHC staining and analysis by Chi-square test. ( E ) USP19 protein levels in GC tissues and ANTs were analyzed by IHC staining (200× magnification). High or low expression of USP19 protein in ANTs (a and b); high or low expression of USP19 protein in intestinal-type GC (c and d); high or low expression of USP19 protein in diffuse-type GC (e and f), Scale bar=200 μm.

Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: Relative expression of USP19 in GC cell lines and GES1. ( A ) Differential expression of USP19 mRNA in gastric cell lines by real-time PCR assay. ( B ) The differential expression of USP19 protein in gastric cell lines by WB analysis. ( C ) Differential expression and distribution of USP19 protein in gastric cell lines by confocal analysis, Scale bar=20 μm. ( D ) Relative expression of USP19 in paired 212 GC samples and ANTs by IHC staining and analysis by Chi-square test. ( E ) USP19 protein levels in GC tissues and ANTs were analyzed by IHC staining (200× magnification). High or low expression of USP19 protein in ANTs (a and b); high or low expression of USP19 protein in intestinal-type GC (c and d); high or low expression of USP19 protein in diffuse-type GC (e and f), Scale bar=200 μm.

Techniques: Expressing, Quantitative Proteomics, Real-time Polymerase Chain Reaction, Immunohistochemistry

The Relationship Between USP19 Expression and Other Clinicopathological Parameters in GC

Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: The Relationship Between USP19 Expression and Other Clinicopathological Parameters in GC

Techniques: Expressing

Kaplan–Meier survival curves of GC patients according to the clinical features and expression of USP19. ( A ) Patients with high-level USP19 expression showed poor survival. ( B ) TNM stage was a promising indicator of the prognosis of GC patients. ( C ) Patients with intestinal-type GC had better survival than those with diffuse-type GC. ( D ) Low-level USP19 expression predicted better survival in patients with TNM stage III. ( E ) Low-level USP19 expression predicted better survival in patients with diffuse-type GC. ( F ) Low-level USP19 expression predicted better survival in patients with intestinal-type GC.

Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: Kaplan–Meier survival curves of GC patients according to the clinical features and expression of USP19. ( A ) Patients with high-level USP19 expression showed poor survival. ( B ) TNM stage was a promising indicator of the prognosis of GC patients. ( C ) Patients with intestinal-type GC had better survival than those with diffuse-type GC. ( D ) Low-level USP19 expression predicted better survival in patients with TNM stage III. ( E ) Low-level USP19 expression predicted better survival in patients with diffuse-type GC. ( F ) Low-level USP19 expression predicted better survival in patients with intestinal-type GC.

Techniques: Expressing

Multivariate Cox Model for the Association Between Survival and Clinicopathological Factors in GC

Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: Multivariate Cox Model for the Association Between Survival and Clinicopathological Factors in GC

Techniques: Expressing

Functional roles of USP19 in cell models. ( A ) Knockdown of USP19 inhibited SGC7901 cell growth, as determined by MTT assay. Bars: SD; **P<0.01. ( B ) Knockdown of USP19 decreased the level of MMP2 and MMP9 and increased the level of the apoptotic-related protein, cleaved caspase-3, in SGC7901 cells. ( C ) Knockdown of USP19 inhibited colony formation in SGC7901 cells. The data are shown as Mean±SD (n=3). **P<0.01. ( D ) Ectopic expression of USP19 promoted GES1 cell growth, as shown by MTT assay. Bars: SD; **P<0.01. ( E ) Overexpression of USP19 increased the protein level of MMP2 and MMP9 and decreased the level of the apoptotic-related protein, cleaved caspase-3. ( F ) Ectopic expression of USP19 increased colony formation in GES1 cells. The data are shown as Mean±SD (n=3). **P<0.01. All WB experiments were repeated three times.

Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: Functional roles of USP19 in cell models. ( A ) Knockdown of USP19 inhibited SGC7901 cell growth, as determined by MTT assay. Bars: SD; **P<0.01. ( B ) Knockdown of USP19 decreased the level of MMP2 and MMP9 and increased the level of the apoptotic-related protein, cleaved caspase-3, in SGC7901 cells. ( C ) Knockdown of USP19 inhibited colony formation in SGC7901 cells. The data are shown as Mean±SD (n=3). **P<0.01. ( D ) Ectopic expression of USP19 promoted GES1 cell growth, as shown by MTT assay. Bars: SD; **P<0.01. ( E ) Overexpression of USP19 increased the protein level of MMP2 and MMP9 and decreased the level of the apoptotic-related protein, cleaved caspase-3. ( F ) Ectopic expression of USP19 increased colony formation in GES1 cells. The data are shown as Mean±SD (n=3). **P<0.01. All WB experiments were repeated three times.

Techniques: Functional Assay, Knockdown, MTT Assay, Expressing, Over Expression

Effect of USP19 on cell invasion and migration and MMP2/MMP9 enzyme activity. ( A ) Knockdown of USP19 reduced SGC7901 cell invasion. Images were acquired at the indicated time (200× magnification). Data are shown as Mean±SD (n=5). **P<0.01. ( B ) Knockdown of USP19 reduced the migration of SGC7901 cells, as determined by wound healing assay at 0, 12, 24, and 36 h. Images were acquired at the indicated time (100× magnification). Data are shown as Mean±SD (n=3). **P<0.01. ( C ) SGC7901 cells were transfected with ShUSP19 or vector for 36 h and then subjected to gelatin zymography to analyze the activity of MMP2 and MMP9. ( D ) Ectopic expression of USP19 enhanced invasion of GES1 cells. Images were acquired at the indicated time (200× magnification). Data are shown as Mean±SD (n=5). **P<0.01. ( E ) Ectopic expression of USP19 increased the migration of GES1 cells, as determined by wound healing assay at 0, 12, 24, and 36 h. Images were acquired at the indicated time (100× magnification). Data are shown as Mean±SD (n=3). **P<0.01. ( F ) GES1 cells were transfected with USP19-overexpressing plasmid or control vector for 36 h and then subjected to gelatin zymography to analyze the activity of MMP2 and MMP9.

Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: Effect of USP19 on cell invasion and migration and MMP2/MMP9 enzyme activity. ( A ) Knockdown of USP19 reduced SGC7901 cell invasion. Images were acquired at the indicated time (200× magnification). Data are shown as Mean±SD (n=5). **P<0.01. ( B ) Knockdown of USP19 reduced the migration of SGC7901 cells, as determined by wound healing assay at 0, 12, 24, and 36 h. Images were acquired at the indicated time (100× magnification). Data are shown as Mean±SD (n=3). **P<0.01. ( C ) SGC7901 cells were transfected with ShUSP19 or vector for 36 h and then subjected to gelatin zymography to analyze the activity of MMP2 and MMP9. ( D ) Ectopic expression of USP19 enhanced invasion of GES1 cells. Images were acquired at the indicated time (200× magnification). Data are shown as Mean±SD (n=5). **P<0.01. ( E ) Ectopic expression of USP19 increased the migration of GES1 cells, as determined by wound healing assay at 0, 12, 24, and 36 h. Images were acquired at the indicated time (100× magnification). Data are shown as Mean±SD (n=3). **P<0.01. ( F ) GES1 cells were transfected with USP19-overexpressing plasmid or control vector for 36 h and then subjected to gelatin zymography to analyze the activity of MMP2 and MMP9.

Techniques: Migration, Activity Assay, Knockdown, Wound Healing Assay, Transfection, Plasmid Preparation, Zymography, Expressing, Control

Knockdown of USP19 reduced gastric carcinogenesis in animal models. Knockdown of USP19 inhibited tumor growth. Five nude mice were injected subcutaneously with 1 × 10 7 cells/mouse for each of the indicated stable cell lines of SGC7901-ShUSP19 or SGC7901-Vector, respectively. Results were presented as tumor volume at the different time points ( C ) and isolated xenografts at day 24 ( A ) with no change in body weight for each group ( B ). **P<0.01. ( D ) Tumors were isolated, fixed, and subjected to IHC assay (400× magnification).

Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: Knockdown of USP19 reduced gastric carcinogenesis in animal models. Knockdown of USP19 inhibited tumor growth. Five nude mice were injected subcutaneously with 1 × 10 7 cells/mouse for each of the indicated stable cell lines of SGC7901-ShUSP19 or SGC7901-Vector, respectively. Results were presented as tumor volume at the different time points ( C ) and isolated xenografts at day 24 ( A ) with no change in body weight for each group ( B ). **P<0.01. ( D ) Tumors were isolated, fixed, and subjected to IHC assay (400× magnification).

Techniques: Knockdown, Injection, Stable Transfection, Plasmid Preparation, Isolation

Prognostic roles of USP19 was determined in www.kmplot.com and correlation between mRNA levels of USP19 and MMP2/MMP9 in GC dataset from GEPIA Website ( http://gepia2.cancer-pku.cn ). Overall survival curves were plotted for all GC patients (n=876) with ( A ) different levels of USP19 expression; ( B ) Lauren’s classification; ( C and D ) HER2 status, ( E ) Different treatments, 5-FU based chemical therapy, ( F ) Surgery alone. From these data, low level of USP19 expression was correlated with better OS. The mRNA level of USP19 expression was positive association with MMP2/MMP9 expression. ( G) P<0.001 correlation between MMP9 and USP19; ( H ) P=0.062, correlation between MMP2 and USP19. Especially, the moderate correlation was performed between USP19 and MMP9 expression (Spearman’s R=0.24).

Journal: OncoTargets and therapy

Article Title: USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

doi: 10.2147/OTT.S240543

Figure Lengend Snippet: Prognostic roles of USP19 was determined in www.kmplot.com and correlation between mRNA levels of USP19 and MMP2/MMP9 in GC dataset from GEPIA Website ( http://gepia2.cancer-pku.cn ). Overall survival curves were plotted for all GC patients (n=876) with ( A ) different levels of USP19 expression; ( B ) Lauren’s classification; ( C and D ) HER2 status, ( E ) Different treatments, 5-FU based chemical therapy, ( F ) Surgery alone. From these data, low level of USP19 expression was correlated with better OS. The mRNA level of USP19 expression was positive association with MMP2/MMP9 expression. ( G) P<0.001 correlation between MMP9 and USP19; ( H ) P=0.062, correlation between MMP2 and USP19. Especially, the moderate correlation was performed between USP19 and MMP9 expression (Spearman’s R=0.24).

Techniques: Expressing

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