Journal: Cell Stem Cell

Article Title: Sphingolipid Modulation Activates Proteostasis Programs to Govern Human Hematopoietic Stem Cell Self-Renewal

doi: 10.1016/j.stem.2019.09.008

Figure Lengend Snippet:

Article Snippet: TPO , Milteny Biotec , 130-095-752.

Techniques: Recombinant, SYBR Green Assay, shRNA, Amplification, Sequencing, Control, Plasmid Preparation, Software, Modification

Journal: International Journal of Molecular Sciences

Article Title: KIT D816V Mast Cells Derived from Induced Pluripotent Stem Cells Recapitulate Systemic Mastocytosis Transcriptional Profile

doi: 10.3390/ijms24065275

Figure Lengend Snippet: Media composition for iPS cell differentiation toward mast cells.

Article Snippet: , SCF , 50 ng/ml , 130-095-745 , Miltenyi , , , , , .

Techniques: Cell Differentiation, Concentration Assay

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Human adipose tissue-derived stromal cells can differentiate into megakaryocytes and platelets by secreting endogenous thrombopoietin.

doi: 10.1111/jth.13313

Figure Lengend Snippet: Fig. 2. Effect of endogenous thrombopoietin (TPO) on megakaryocyte (MK) differentiation from adipose tissue-derived stromal cells (ASCs). (A) Representative data of CD41/42b expression in MKs derived from human ASCs (hASCs) cultured in MK lineage induction (MKLI) med- ium for 14 days. (B) Representative data of DNA polyploidy in MKs derived from hASCs cultured in MKLI medium for 14 days. (C) Repre- sentative data of CD29, CD61 and CD49b expression in platelet samples, peripheral platelets and platelets derived from hASCs cultured in MKLI medium for 21 days in the presence or absence of recombinant TPO (rTPO). The red line indicates the isotype control. (D) Representa- tive data of c-MPL expression in mouse ASCs. Bar: 20 lm. (E) MK production from mouse ASCs (4 9 105) cultured in MKLI medium for 7 days in the presence (5 lg mL1 and 10 lg mL1) or absence of anti-mouse c-MPL blocking antibody AMM2 (*P < 0.05). (F) MK produc- tion from mouse ASCs (4 9 105) cultured in MKLI medium for 2, 4 and 6 days in the presence (10 lg mL1) or absence of anti-mouse c- MPL blocking antibody AMM2. **P < 0.01. DAPI, 40,6-diamidino-2-phenylindole; hPLT, human peripheral platelet; NS, not significant; rTPO(+), MKLI medium with rTPO; rTPO(–), MKLI medium without rTPO.

Article Snippet: Briefly, anti-human CD71 primary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA), anti-human TPO primary antibodies (R&D Systems), anti-human b-actin (Abcam, Cambridge, UK) and horseradish peroxidase-conjugated secondary antibody (Dako) were used.

Techniques: Derivative Assay, Expressing, Cell Culture, Recombinant, Control, Blocking Assay

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Human adipose tissue-derived stromal cells can differentiate into megakaryocytes and platelets by secreting endogenous thrombopoietin.

doi: 10.1111/jth.13313

Figure Lengend Snippet: Fig. 6. Effect of transferrin receptor CD71 on thrombopoietin (TPO) production from human adipose tissue-derived stromal cells (hASCs). (A) hASCs were cultured in megakaryocyte lineage induction (MKLI) transferrin medium in the presence (final concentration, 10 lg mL1) or absence of anti-human CD71 blocking antibody for 6 days. (a–c) The effect of anti-human CD71 blocking antibody on (a) CD71 gene expres- sion, (b) CD71 protein expression, and (c) cell viability. (d, e) The effect of anti-human CD71 blocking antibody on (d) TPO gene expression and (e) TPO protein expression. (B) hASCs transfected with small interfering RNA (siRNA) against CD71 (siRNA-CD71) or control siRNA were cultured in MKLI transferrin medium for 6 days. (a–d) The effects of siRNA against human CD71 on (a) CD71 gene expression, (b) CD71 protein expression, (c) CD71 surface expression, and (d) cell viability. (e, f) The effects of siRNA against human CD71 on (e) TPO gene expression and (f) TPO protein expression. *P < 0.05, **P < 0.01. (C) TPO gene expression and TPO protein production from CD71-positive and CD71-negative hASCs. Each cell population was obtained by fluorescence-activated cell sorting, and was then cultured in MKLI transfer- rin medium for 6 days. *P < 0.05, **P < 0.01. (D) TPO production from CD71-negative hASCs cultured in MKLI transferrin medium in the presence or absence of anti-human interleukin (IL)-6 antibody for 6 days. ND, not detectable; NS, not significant.

Article Snippet: Briefly, anti-human CD71 primary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA), anti-human TPO primary antibodies (R&D Systems), anti-human b-actin (Abcam, Cambridge, UK) and horseradish peroxidase-conjugated secondary antibody (Dako) were used.

Techniques: Derivative Assay, Cell Culture, Concentration Assay, Blocking Assay, Expressing, Gene Expression, Transfection, Small Interfering RNA, Control, FACS