Journal: Cancers

Article Title: Testicular Germ Cell Tumors Acquire Cisplatin Resistance by Rebalancing the Usage of DNA Repair Pathways

doi: 10.3390/cancers13040787

Figure Lengend Snippet: List of the antibodies, commercial sources and dilutions used in this study.

Article Snippet: TP53BP1 , OriGene , TA309918 , 1:1000.

Techniques:

Journal: Stem Cells International

Article Title: Dual Effects of Hypoxia-Inducible Factors-1 Alpha in Bleomycin-Induced Pulmonary Fibrosis Treated by Human Umbilical Cord Mesenchymal Stem Cells

doi: 10.1155/2021/6658855

Figure Lengend Snippet: Preventive administration of HUCMSCs can effectively attenuate pulmonary fibrosis induced by bleomycin in A549 cells. (a) Results of immunofluorescence staining in A549 cells of the control, CM-pretreatment, CM-treatment, belomycin, belomycin+CM-pretreatment, and belomycin+CM-treatment groups. TP53BP1 are stained in A549 cells with antibodies (red). Nuclei are stained with DAPI (blue). Scale bar = 100 μ m. (b) Results of western blot in the same groups. (c) Results of qPCR in the same groups. Data are presented as mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 vs. the control; # p ≤ 0.05, ## p ≤ 0.01 vs. the model.

Article Snippet: The cells were blocked in 5%BSA for 1 h and then incubated with primary antibody at 4°C overnight against TP53BP1 (Boster, BA2878).

Techniques: Immunofluorescence, Staining, Control, Western Blot

Journal: Chromosoma

Article Title: DNA repair kinetics in SCID mice Sertoli cells and DNA-PKcs-deficient mouse embryonic fibroblasts

doi: 10.1007/s00412-016-0590-9

Figure Lengend Snippet: DSB damage persistence before IR and repair kinetics after IR in Sertoli cells. a Testicular section of a wild-type mouse showing no 53BP1 DSB-indicating foci in Sertoli cells. Sertoli cells are characterized by irregular-shaped nucleus with two blue dots next to the dark spot (the nucleolus) representing the chromocenters that are specific for this cell type. b 53BP1 foci ( red ) in Prdkc scid Sertoli cells ( arrows ). c–f Representative images for IR-induced 53BP1 foci in wild-type and SCID Sertoli cells. g , h γ-H2AX foci in nonirradiated Prdkc scid Sertoli cells ( arrows in G indicate green γ-H2AX foci and arrows in H show co-localized foci). i Irradiated wild-type testis ( arrows show co-localized foci). j Prdkc scid Sertoli cells displaying γH2AX foci co-localized with 53BP1 ( arrows ). S Sertoli, Sc spermatocyte, B type B spermatogonia, A type A spermatogonia, eS early spermatocytes. Scale bars represent 10 μm

Article Snippet: The primary antibodies used were mouse monoclonal anti-proliferating cell nuclear antigen (PCNA; Merck) (1:200 in blocking buffer), rabbit polyclonal anti-53BP1 (1:400; Acris Antibodies), and mouse monoclonal antibody anti-γ-H2AX (1:500, JBW301, Millipore).

Techniques: Irradiation

Journal: Chromosoma

Article Title: DNA repair kinetics in SCID mice Sertoli cells and DNA-PKcs-deficient mouse embryonic fibroblasts

doi: 10.1007/s00412-016-0590-9

Figure Lengend Snippet: DNA repair kinetics after IR in Sertoli cells of wild-type and Prdkc scid mice. a Percentages of Sertoli cells with foci at different time points after 0.5 Gy gamma-IR. a Average number of 53BP1 foci per Sertoli cell before and at different time points after IR. Fifty cells per mouse were analyzed per time point (three mice each)

Article Snippet: The primary antibodies used were mouse monoclonal anti-proliferating cell nuclear antigen (PCNA; Merck) (1:200 in blocking buffer), rabbit polyclonal anti-53BP1 (1:400; Acris Antibodies), and mouse monoclonal antibody anti-γ-H2AX (1:500, JBW301, Millipore).

Techniques:

Journal: Chromosoma

Article Title: DNA repair kinetics in SCID mice Sertoli cells and DNA-PKcs-deficient mouse embryonic fibroblasts

doi: 10.1007/s00412-016-0590-9

Figure Lengend Snippet: Telomeres and DSB damage foci at Sertoli cells before and after IR. a – f Representative images form nonirradiated a , b and irradiated d – f wild-type and Prdkc scid testes showing the co-localization of 53BP1 foci at telomeres of TD Sertoli cells ( arrow heads ). g Percentages of 53BP1 foci that overlap (partially or co-localize) with telomere signals before and after IR. S Sertoli, Sc spermatocyte, B type B spermatogonia, A type A spermatogonia, eS early spermatocytes. Scale bars at 10 μm

Article Snippet: The primary antibodies used were mouse monoclonal anti-proliferating cell nuclear antigen (PCNA; Merck) (1:200 in blocking buffer), rabbit polyclonal anti-53BP1 (1:400; Acris Antibodies), and mouse monoclonal antibody anti-γ-H2AX (1:500, JBW301, Millipore).

Techniques: Irradiation

Journal: Chromosoma

Article Title: DNA repair kinetics in SCID mice Sertoli cells and DNA-PKcs-deficient mouse embryonic fibroblasts

doi: 10.1007/s00412-016-0590-9

Figure Lengend Snippet: Immunofluorescent analysis of different cell cycle phases in 53BP1-stained wild-type, DNA-PKcs −/− and Ku −/− MEF cell lines. Cells were synchronized by serum starvation for 18 h. a Substages of S-phase cells shown according to PCNA ( green ) staining patterns; G 1 and G 2 /M phases are negative for PCNA. b Representative images showing the presence, induction, and disappearance of 53BP1 DSB-indicating foci after IR in wild-type, DNA-PKcs −/− , and Ku −/− MEF cells 0 h, 5 min, 30 min, 1 h, and 7 h post-IR. Note the increase in foci after 7 h of IR in DNA-PKcs −/− cells

Article Snippet: The primary antibodies used were mouse monoclonal anti-proliferating cell nuclear antigen (PCNA; Merck) (1:200 in blocking buffer), rabbit polyclonal anti-53BP1 (1:400; Acris Antibodies), and mouse monoclonal antibody anti-γ-H2AX (1:500, JBW301, Millipore).

Techniques: Staining

Journal: Chromosoma

Article Title: DNA repair kinetics in SCID mice Sertoli cells and DNA-PKcs-deficient mouse embryonic fibroblasts

doi: 10.1007/s00412-016-0590-9

Figure Lengend Snippet: Radiation-induced 53BP1 foci (RIF) in wild-type and mutant MEF cell lines. a The kinetics of induction and loss of 53BP1 foci in sham-irradiated and X-irradiated wild-type, DNA-PKcs −/− , and Ku −/− MEFs (in G 1 -phase) 5 min, 30 min, 1 h, 3 h, and 7 h post-IR. b Repair kinetics after applying a correction factor based on the FACS analysis and the DI of cell lines. c Repair kinetics in S-phase cells and background foci (at 0 h) were subtracted and foci values were normalized according to the DI. Compared to the wild type ( a ), compared to the Ku −/− ( b ), and compared to DNA-PKcs −/− ( c ). * p < 0.05

Article Snippet: The primary antibodies used were mouse monoclonal anti-proliferating cell nuclear antigen (PCNA; Merck) (1:200 in blocking buffer), rabbit polyclonal anti-53BP1 (1:400; Acris Antibodies), and mouse monoclonal antibody anti-γ-H2AX (1:500, JBW301, Millipore).

Techniques: Mutagenesis, Irradiation