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Image Search Results
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Structure-based design of a soluble human cytomegalovirus glycoprotein B antigen stabilized in a prefusion-like conformation
doi: 10.1073/pnas.2404250121
Figure Lengend Snippet: Engineered gB variants are immunogenic in mice. ( A ) Schematic of mouse immunization. 6-to-8-week-old female BALB/c mice ( n = 8/group) were immunized at weeks 0, 3, and 6 with 2.5 μg of gB Base (orange), gB V134C/I653C (red), or gB-C7 (green), all adjuvanted with CpG 1018 plus alum or unadjuvanted gB-C7 (purple). Blood samples were collected from mice 2 wk after the week 6 injection. SM5-1-equivalent antibody concentrations of immunized mouse sera that bind to ( B ) gB Base or ( C ) gB-C7. Plots represent ELISA measurements relative to an SM5-1 mFc standard from two experimental replicates. AD169-GFP infection of human MRC-5 fibroblasts. ( D ) Neutralization by Cytogam is enhanced 3.6-fold in the presence of 12.5% guinea pig complement. Data are averaged across 16 plates with SD shown. Neutralization by mouse sera in the ( E ) absence and ( F ) presence of 12.5% guinea pig complement. Plots represent averages from two independent experiments. In violin plots, horizontal lines represent the first quartile, median, and third quartile. Statistical significance was determined by one-way ANOVA followed by Tukey’s HSD test in GraphPad Prism: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
Article Snippet: For assays including complement,
Techniques: Injection, Enzyme-linked Immunosorbent Assay, Infection, Neutralization