Journal: Cell Death & Disease

Article Title: Alternative splicing of BAZ1A in colorectal cancer disrupts the DNA damage response and increases chemosensitization

doi: 10.1038/s41419-024-06954-6

Figure Lengend Snippet: A Immunoblotting in HCT116 cells after stable transfection with scrambled shCtrl or three different shRNAs targeting BAZ1A, with β-Actin as loading control. B RT-qPCR analysis of BAZ1A , CTNNB1 , CCAR2 , and c- MYC in shCtrl and shBAZ1A cells. C shCtrl and shBAZ1A KD cells were transiently transfected with TCF/LEF-responsive luciferase reporter construct TOPFlash or negative control FOPFlash vectors, along with Renilla pRL-TK plasmid to correct for transfection efficiency. D BAZ1A interactions on c- MYC were examined using ChIP assays in parental HCT116 CCAR2 +/+ or HCT116 CCAR2 -/- cells, with IgG serving as negative control. Statistical significance determined by Student’s t-test for n = 3 replicates, indicated by ***p < 0.001, ****p < 0.0001 vs. shCtrl.

Article Snippet: BAZ1A KD cells (see above) were transiently transfected with 1 μg of plasmid DNA for the Wnt reporter construct TOPflash, or the negative control FOPflash (Upstate Biotechnology, Lake Placid, NY, USA).

Techniques: Western Blot, Stable Transfection, Control, Quantitative RT-PCR, Transfection, Luciferase, Construct, Negative Control, Plasmid Preparation