tom20 aip Search Results


tom20 aip  (Addgene inc)
91
Addgene inc tom20 aip
(A) Colocalization analysis and (B) immunofluorescence image of LAMP1 and AMPK signals in CTLs. Quantitative measurements were taken for 30 CTLs (n=3 donors). (C) Immunofluorescence images of CTLs electroporated with LAMP1-AIP-mCherry or <t>Tom20-AIP-mCherry</t> constructs and stained with antibodies against perforin (granules). (D) Quantitative analysis of lytic granule polarization in antibody-stimulated mCherry-positive CTLs overexpressing either LAMP1-AIP-mCherry or Tom20-AIP-mCherry (n=3 experiments). (E) Colocalization analysis of immunofluorescence signals in CTLs stained with antibodies against AMPK and perforin (granules) (n=3 experiments). (F) Immunofluorescence image of a CTL stained with antibodies against AMPK, LAMP1 and perforin (granules). (G) CLEM analysis of lytic granules and AMPK-positive compartment in freshly isolated CTLs. N, nucleus; LD, lipid droplet; AMPK, AMPK-positive vesicle; LG, lytic granule. Scale bar, 5 μm. Shown are mean values ± SD; ns, not significant; two-way ANOVA test ****p < 0.0001.
Tom20 Aip, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom20 aip/product/Addgene inc
Average 91 stars, based on 1 article reviews
tom20 aip - by Bioz Stars, 2026-03
91/100 stars
  Buy from Supplier
tom20 mchf aip  (Addgene inc)
92
Addgene inc tom20 mchf aip
(A) Colocalization analysis and (B) immunofluorescence image of LAMP1 and AMPK signals in CTLs. Quantitative measurements were taken for 30 CTLs (n=3 donors). (C) Immunofluorescence images of CTLs electroporated with LAMP1-AIP-mCherry or <t>Tom20-AIP-mCherry</t> constructs and stained with antibodies against perforin (granules). (D) Quantitative analysis of lytic granule polarization in antibody-stimulated mCherry-positive CTLs overexpressing either LAMP1-AIP-mCherry or Tom20-AIP-mCherry (n=3 experiments). (E) Colocalization analysis of immunofluorescence signals in CTLs stained with antibodies against AMPK and perforin (granules) (n=3 experiments). (F) Immunofluorescence image of a CTL stained with antibodies against AMPK, LAMP1 and perforin (granules). (G) CLEM analysis of lytic granules and AMPK-positive compartment in freshly isolated CTLs. N, nucleus; LD, lipid droplet; AMPK, AMPK-positive vesicle; LG, lytic granule. Scale bar, 5 μm. Shown are mean values ± SD; ns, not significant; two-way ANOVA test ****p < 0.0001.
Tom20 Mchf Aip, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom20 mchf aip/product/Addgene inc
Average 92 stars, based on 1 article reviews
tom20 mchf aip - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier

Image Search Results


(A) Colocalization analysis and (B) immunofluorescence image of LAMP1 and AMPK signals in CTLs. Quantitative measurements were taken for 30 CTLs (n=3 donors). (C) Immunofluorescence images of CTLs electroporated with LAMP1-AIP-mCherry or Tom20-AIP-mCherry constructs and stained with antibodies against perforin (granules). (D) Quantitative analysis of lytic granule polarization in antibody-stimulated mCherry-positive CTLs overexpressing either LAMP1-AIP-mCherry or Tom20-AIP-mCherry (n=3 experiments). (E) Colocalization analysis of immunofluorescence signals in CTLs stained with antibodies against AMPK and perforin (granules) (n=3 experiments). (F) Immunofluorescence image of a CTL stained with antibodies against AMPK, LAMP1 and perforin (granules). (G) CLEM analysis of lytic granules and AMPK-positive compartment in freshly isolated CTLs. N, nucleus; LD, lipid droplet; AMPK, AMPK-positive vesicle; LG, lytic granule. Scale bar, 5 μm. Shown are mean values ± SD; ns, not significant; two-way ANOVA test ****p < 0.0001.

Journal: bioRxiv

Article Title: Phosphoproteomics of CD2 signaling reveals an AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells

doi: 10.1101/795963

Figure Lengend Snippet: (A) Colocalization analysis and (B) immunofluorescence image of LAMP1 and AMPK signals in CTLs. Quantitative measurements were taken for 30 CTLs (n=3 donors). (C) Immunofluorescence images of CTLs electroporated with LAMP1-AIP-mCherry or Tom20-AIP-mCherry constructs and stained with antibodies against perforin (granules). (D) Quantitative analysis of lytic granule polarization in antibody-stimulated mCherry-positive CTLs overexpressing either LAMP1-AIP-mCherry or Tom20-AIP-mCherry (n=3 experiments). (E) Colocalization analysis of immunofluorescence signals in CTLs stained with antibodies against AMPK and perforin (granules) (n=3 experiments). (F) Immunofluorescence image of a CTL stained with antibodies against AMPK, LAMP1 and perforin (granules). (G) CLEM analysis of lytic granules and AMPK-positive compartment in freshly isolated CTLs. N, nucleus; LD, lipid droplet; AMPK, AMPK-positive vesicle; LG, lytic granule. Scale bar, 5 μm. Shown are mean values ± SD; ns, not significant; two-way ANOVA test ****p < 0.0001.

Article Snippet: For overexpression of LAMP1-AIP and Tom20-AIP, CTLs were nucleofected with 2 μg of purified LAMP-mChF-AIP and Tom20-mChF-AIP plasmids (a gift from Takanari Inoue, Addgene plasmids # 61524 and # 61512).

Techniques: Immunofluorescence, Construct, Staining, Isolation