tnt assay kit Search Results


tnt kit  (Promega)
90
Promega tnt kit
Tnt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/tnt+kit/us06864236-1353-13-12
Average 90 stars, based on 1 article reviews
tnt kit - by Bioz Stars, 2026-09
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promega tnt system  (Promega)
90
Promega promega tnt system
Promega Tnt System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/promega+tnt+kit/10__1128_slash_mcb__20__15__5722___5735__2000-110-26-25
Average 90 stars, based on 1 article reviews
promega tnt system - by Bioz Stars, 2026-09
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rrl transcription and translation system tnt quick coupled  (Promega)
90
Promega rrl transcription and translation system tnt quick coupled
35 S-methionine-labeled Sir4 was synthesized in vitro in a rabbit reticulocyte lysate <t>transcription/translation</t> system <t>(RRL)</t> to which purified Ku heterodimer, bearing a 2myc epitope on the C-terminus of Yku80, was added. After Sir4 synthesis, the RRL was subjected to anti-myc immunoprecipitation. The input, unbound, and bound fractions were run on an SDS polyacrylamide gel, which was imaged by autoradiography. DOI: http://dx.doi.org/10.7554/eLife.07750.010
Rrl Transcription And Translation System Tnt Quick Coupled, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/tnt+rrl+kit/pmc04547093-306-16-24
Average 90 stars, based on 1 article reviews
rrl transcription and translation system tnt quick coupled - by Bioz Stars, 2026-09
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tnt-quick kit  (Promega)
90
Promega tnt-quick kit
35 S-methionine-labeled Sir4 was synthesized in vitro in a rabbit reticulocyte lysate <t>transcription/translation</t> system <t>(RRL)</t> to which purified Ku heterodimer, bearing a 2myc epitope on the C-terminus of Yku80, was added. After Sir4 synthesis, the RRL was subjected to anti-myc immunoprecipitation. The input, unbound, and bound fractions were run on an SDS polyacrylamide gel, which was imaged by autoradiography. DOI: http://dx.doi.org/10.7554/eLife.07750.010
Tnt Quick Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/tnt+quick+kit/10__1042_slash_bj20031525-67-7-21
Average 90 stars, based on 1 article reviews
tnt-quick kit - by Bioz Stars, 2026-09
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t3/t7coupled tnt expression kits  (Promega)
90
Promega t3/t7coupled tnt expression kits
35 S-methionine-labeled Sir4 was synthesized in vitro in a rabbit reticulocyte lysate <t>transcription/translation</t> system <t>(RRL)</t> to which purified Ku heterodimer, bearing a 2myc epitope on the C-terminus of Yku80, was added. After Sir4 synthesis, the RRL was subjected to anti-myc immunoprecipitation. The input, unbound, and bound fractions were run on an SDS polyacrylamide gel, which was imaged by autoradiography. DOI: http://dx.doi.org/10.7554/eLife.07750.010
T3/T7coupled Tnt Expression Kits, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/tnt+expression+kit/10__1074_slash_jbc__271__29__17547-44-0-7
Average 90 stars, based on 1 article reviews
t3/t7coupled tnt expression kits - by Bioz Stars, 2026-09
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cell free tnt quick coupled transcription/translation kit  (Promega)
90
Promega cell free tnt quick coupled transcription/translation kit
35 S-methionine-labeled Sir4 was synthesized in vitro in a rabbit reticulocyte lysate <t>transcription/translation</t> system <t>(RRL)</t> to which purified Ku heterodimer, bearing a 2myc epitope on the C-terminus of Yku80, was added. After Sir4 synthesis, the RRL was subjected to anti-myc immunoprecipitation. The input, unbound, and bound fractions were run on an SDS polyacrylamide gel, which was imaged by autoradiography. DOI: http://dx.doi.org/10.7554/eLife.07750.010
Cell Free Tnt Quick Coupled Transcription/Translation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/cell+free+translation+system+tnt+kit/pmc04406435-346-21-28
Average 90 stars, based on 1 article reviews
cell free tnt quick coupled transcription/translation kit - by Bioz Stars, 2026-09
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reticulocyte extract tnt kit  (Promega)
90
Promega reticulocyte extract tnt kit
(A, B) GST-XRLIP or GST alone as control were tested for their ability to pull down Xreps1(253) or Xreps1(RLIP-BD) synthesized as Myc-tagged proteins. The interaction of XRLIP with RalB G23V served as positive control. Xreps1(253) precipitated with 1 µg GST-XRLIP was analyzed. (A) The input or supernatant ranged from 1.25 µl of the 25 µl of <t>reticulocyte</t> extract), or (B) half an embryo from extracts of 10 embryos, were analyzed by SDS-PAGE. (C) Myc-Xreps1(253) or Myc-Xreps1(RLIP-BD) were synthesized in embryos and used to interact with endogenous XRLIP. Total lysate from 10 embryos were incubated with an anti-Myc agarose-conjugated antibody (SC-40, Santa Cruz Biotechnology) and centrifuged. The supernatant and immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-RLIP, or anti-Myc antibodies. With Myc-Xreps1(RLIP-BD), the RLIP signal was weakly detected just above an unspecific signal (<). (D) Two µg of GST-Xreps1(253), GST-Xreps1(RLIP-BD), or 1 µg of GST-XralA (G23V) preloaded using GTP-g-S, or GST alone were incubated with Myc-XRLIP (lysate from 10 embryos) and purified with glutathione-agarose beads. The precipitated proteins were resolved by SDS-PAGE, transferred onto a Hybond-P membrane (Amersham) and blotted with a monoclonal anti-Myc antibody (9E10). (E) The table recapitulates the results obtained on the in vivo and in vitro interaction of XRLIP with Xreps1.
Reticulocyte Extract Tnt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/reticulocyte+extract+tnt+kit/pmc03297634-265-11-15
Average 90 stars, based on 1 article reviews
reticulocyte extract tnt kit - by Bioz Stars, 2026-09
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proteins produced by in vitro coupled transcription-translation reactions tnt kit  (Promega)
90
Promega proteins produced by in vitro coupled transcription-translation reactions tnt kit
(A, B) GST-XRLIP or GST alone as control were tested for their ability to pull down Xreps1(253) or Xreps1(RLIP-BD) synthesized as Myc-tagged proteins. The interaction of XRLIP with RalB G23V served as positive control. Xreps1(253) precipitated with 1 µg GST-XRLIP was analyzed. (A) The input or supernatant ranged from 1.25 µl of the 25 µl of <t>reticulocyte</t> extract), or (B) half an embryo from extracts of 10 embryos, were analyzed by SDS-PAGE. (C) Myc-Xreps1(253) or Myc-Xreps1(RLIP-BD) were synthesized in embryos and used to interact with endogenous XRLIP. Total lysate from 10 embryos were incubated with an anti-Myc agarose-conjugated antibody (SC-40, Santa Cruz Biotechnology) and centrifuged. The supernatant and immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-RLIP, or anti-Myc antibodies. With Myc-Xreps1(RLIP-BD), the RLIP signal was weakly detected just above an unspecific signal (<). (D) Two µg of GST-Xreps1(253), GST-Xreps1(RLIP-BD), or 1 µg of GST-XralA (G23V) preloaded using GTP-g-S, or GST alone were incubated with Myc-XRLIP (lysate from 10 embryos) and purified with glutathione-agarose beads. The precipitated proteins were resolved by SDS-PAGE, transferred onto a Hybond-P membrane (Amersham) and blotted with a monoclonal anti-Myc antibody (9E10). (E) The table recapitulates the results obtained on the in vivo and in vitro interaction of XRLIP with Xreps1.
Proteins Produced By In Vitro Coupled Transcription Translation Reactions Tnt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/proteins+produced+by+in+vitro+coupled+transcription+translation+reactions+tnt+kit/10__1128_slash_mcb__26__10__3942___3954__2006-59-18-28
Average 90 stars, based on 1 article reviews
proteins produced by in vitro coupled transcription-translation reactions tnt kit - by Bioz Stars, 2026-09
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tnt linked kit  (Promega)
90
Promega tnt linked kit
(A, B) GST-XRLIP or GST alone as control were tested for their ability to pull down Xreps1(253) or Xreps1(RLIP-BD) synthesized as Myc-tagged proteins. The interaction of XRLIP with RalB G23V served as positive control. Xreps1(253) precipitated with 1 µg GST-XRLIP was analyzed. (A) The input or supernatant ranged from 1.25 µl of the 25 µl of <t>reticulocyte</t> extract), or (B) half an embryo from extracts of 10 embryos, were analyzed by SDS-PAGE. (C) Myc-Xreps1(253) or Myc-Xreps1(RLIP-BD) were synthesized in embryos and used to interact with endogenous XRLIP. Total lysate from 10 embryos were incubated with an anti-Myc agarose-conjugated antibody (SC-40, Santa Cruz Biotechnology) and centrifuged. The supernatant and immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-RLIP, or anti-Myc antibodies. With Myc-Xreps1(RLIP-BD), the RLIP signal was weakly detected just above an unspecific signal (<). (D) Two µg of GST-Xreps1(253), GST-Xreps1(RLIP-BD), or 1 µg of GST-XralA (G23V) preloaded using GTP-g-S, or GST alone were incubated with Myc-XRLIP (lysate from 10 embryos) and purified with glutathione-agarose beads. The precipitated proteins were resolved by SDS-PAGE, transferred onto a Hybond-P membrane (Amersham) and blotted with a monoclonal anti-Myc antibody (9E10). (E) The table recapitulates the results obtained on the in vivo and in vitro interaction of XRLIP with Xreps1.
Tnt Linked Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/tnt+linked+transcription+translation+kit/pm10713134-67-11-14
Average 90 stars, based on 1 article reviews
tnt linked kit - by Bioz Stars, 2026-09
90/100 stars
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tnt wheat germ lysate kit  (Promega)
90
Promega tnt wheat germ lysate kit
(A, B) GST-XRLIP or GST alone as control were tested for their ability to pull down Xreps1(253) or Xreps1(RLIP-BD) synthesized as Myc-tagged proteins. The interaction of XRLIP with RalB G23V served as positive control. Xreps1(253) precipitated with 1 µg GST-XRLIP was analyzed. (A) The input or supernatant ranged from 1.25 µl of the 25 µl of <t>reticulocyte</t> extract), or (B) half an embryo from extracts of 10 embryos, were analyzed by SDS-PAGE. (C) Myc-Xreps1(253) or Myc-Xreps1(RLIP-BD) were synthesized in embryos and used to interact with endogenous XRLIP. Total lysate from 10 embryos were incubated with an anti-Myc agarose-conjugated antibody (SC-40, Santa Cruz Biotechnology) and centrifuged. The supernatant and immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-RLIP, or anti-Myc antibodies. With Myc-Xreps1(RLIP-BD), the RLIP signal was weakly detected just above an unspecific signal (<). (D) Two µg of GST-Xreps1(253), GST-Xreps1(RLIP-BD), or 1 µg of GST-XralA (G23V) preloaded using GTP-g-S, or GST alone were incubated with Myc-XRLIP (lysate from 10 embryos) and purified with glutathione-agarose beads. The precipitated proteins were resolved by SDS-PAGE, transferred onto a Hybond-P membrane (Amersham) and blotted with a monoclonal anti-Myc antibody (9E10). (E) The table recapitulates the results obtained on the in vivo and in vitro interaction of XRLIP with Xreps1.
Tnt Wheat Germ Lysate Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/tnt+wheat+germ+lysate+kit/10__1074_slash_jbc__m305207200-61-16-15
Average 90 stars, based on 1 article reviews
tnt wheat germ lysate kit - by Bioz Stars, 2026-09
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wheat germ tnt kit  (Promega)
90
Promega wheat germ tnt kit
(A, B) GST-XRLIP or GST alone as control were tested for their ability to pull down Xreps1(253) or Xreps1(RLIP-BD) synthesized as Myc-tagged proteins. The interaction of XRLIP with RalB G23V served as positive control. Xreps1(253) precipitated with 1 µg GST-XRLIP was analyzed. (A) The input or supernatant ranged from 1.25 µl of the 25 µl of <t>reticulocyte</t> extract), or (B) half an embryo from extracts of 10 embryos, were analyzed by SDS-PAGE. (C) Myc-Xreps1(253) or Myc-Xreps1(RLIP-BD) were synthesized in embryos and used to interact with endogenous XRLIP. Total lysate from 10 embryos were incubated with an anti-Myc agarose-conjugated antibody (SC-40, Santa Cruz Biotechnology) and centrifuged. The supernatant and immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-RLIP, or anti-Myc antibodies. With Myc-Xreps1(RLIP-BD), the RLIP signal was weakly detected just above an unspecific signal (<). (D) Two µg of GST-Xreps1(253), GST-Xreps1(RLIP-BD), or 1 µg of GST-XralA (G23V) preloaded using GTP-g-S, or GST alone were incubated with Myc-XRLIP (lysate from 10 embryos) and purified with glutathione-agarose beads. The precipitated proteins were resolved by SDS-PAGE, transferred onto a Hybond-P membrane (Amersham) and blotted with a monoclonal anti-Myc antibody (9E10). (E) The table recapitulates the results obtained on the in vivo and in vitro interaction of XRLIP with Xreps1.
Wheat Germ Tnt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/tnt+coupled+wheat+germ+kit/pmc00137593-51-8-25
Average 90 stars, based on 1 article reviews
wheat germ tnt kit - by Bioz Stars, 2026-09
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35 s-labelled human rars tnt kit  (Promega)
90
Promega 35 s-labelled human rars tnt kit
(A, B) GST-XRLIP or GST alone as control were tested for their ability to pull down Xreps1(253) or Xreps1(RLIP-BD) synthesized as Myc-tagged proteins. The interaction of XRLIP with RalB G23V served as positive control. Xreps1(253) precipitated with 1 µg GST-XRLIP was analyzed. (A) The input or supernatant ranged from 1.25 µl of the 25 µl of <t>reticulocyte</t> extract), or (B) half an embryo from extracts of 10 embryos, were analyzed by SDS-PAGE. (C) Myc-Xreps1(253) or Myc-Xreps1(RLIP-BD) were synthesized in embryos and used to interact with endogenous XRLIP. Total lysate from 10 embryos were incubated with an anti-Myc agarose-conjugated antibody (SC-40, Santa Cruz Biotechnology) and centrifuged. The supernatant and immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-RLIP, or anti-Myc antibodies. With Myc-Xreps1(RLIP-BD), the RLIP signal was weakly detected just above an unspecific signal (<). (D) Two µg of GST-Xreps1(253), GST-Xreps1(RLIP-BD), or 1 µg of GST-XralA (G23V) preloaded using GTP-g-S, or GST alone were incubated with Myc-XRLIP (lysate from 10 embryos) and purified with glutathione-agarose beads. The precipitated proteins were resolved by SDS-PAGE, transferred onto a Hybond-P membrane (Amersham) and blotted with a monoclonal anti-Myc antibody (9E10). (E) The table recapitulates the results obtained on the in vivo and in vitro interaction of XRLIP with Xreps1.
35 S Labelled Human Rars Tnt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+assay+kit/35+s+labelled+human+rars+tnt+kit/pmc06912802-97-1-8
Average 90 stars, based on 1 article reviews
35 s-labelled human rars tnt kit - by Bioz Stars, 2026-09
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Image Search Results


35 S-methionine-labeled Sir4 was synthesized in vitro in a rabbit reticulocyte lysate transcription/translation system (RRL) to which purified Ku heterodimer, bearing a 2myc epitope on the C-terminus of Yku80, was added. After Sir4 synthesis, the RRL was subjected to anti-myc immunoprecipitation. The input, unbound, and bound fractions were run on an SDS polyacrylamide gel, which was imaged by autoradiography. DOI: http://dx.doi.org/10.7554/eLife.07750.010

Journal: eLife

Article Title: The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae

doi: 10.7554/eLife.07750

Figure Lengend Snippet: 35 S-methionine-labeled Sir4 was synthesized in vitro in a rabbit reticulocyte lysate transcription/translation system (RRL) to which purified Ku heterodimer, bearing a 2myc epitope on the C-terminus of Yku80, was added. After Sir4 synthesis, the RRL was subjected to anti-myc immunoprecipitation. The input, unbound, and bound fractions were run on an SDS polyacrylamide gel, which was imaged by autoradiography. DOI: http://dx.doi.org/10.7554/eLife.07750.010

Article Snippet: First, ∼16 pmol of purified, myc-tagged yeast Ku heterodimer ( ; ) was added to the RRL transcription and translation system (TNT Quick Coupled, Promega Madison, WI, United States).

Techniques: Labeling, Synthesized, In Vitro, Purification, Immunoprecipitation, Autoradiography

(A, B) GST-XRLIP or GST alone as control were tested for their ability to pull down Xreps1(253) or Xreps1(RLIP-BD) synthesized as Myc-tagged proteins. The interaction of XRLIP with RalB G23V served as positive control. Xreps1(253) precipitated with 1 µg GST-XRLIP was analyzed. (A) The input or supernatant ranged from 1.25 µl of the 25 µl of reticulocyte extract), or (B) half an embryo from extracts of 10 embryos, were analyzed by SDS-PAGE. (C) Myc-Xreps1(253) or Myc-Xreps1(RLIP-BD) were synthesized in embryos and used to interact with endogenous XRLIP. Total lysate from 10 embryos were incubated with an anti-Myc agarose-conjugated antibody (SC-40, Santa Cruz Biotechnology) and centrifuged. The supernatant and immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-RLIP, or anti-Myc antibodies. With Myc-Xreps1(RLIP-BD), the RLIP signal was weakly detected just above an unspecific signal (<). (D) Two µg of GST-Xreps1(253), GST-Xreps1(RLIP-BD), or 1 µg of GST-XralA (G23V) preloaded using GTP-g-S, or GST alone were incubated with Myc-XRLIP (lysate from 10 embryos) and purified with glutathione-agarose beads. The precipitated proteins were resolved by SDS-PAGE, transferred onto a Hybond-P membrane (Amersham) and blotted with a monoclonal anti-Myc antibody (9E10). (E) The table recapitulates the results obtained on the in vivo and in vitro interaction of XRLIP with Xreps1.

Journal: PLoS ONE

Article Title: Identification and Characterization of the RLIP/RALBP1 Interacting Protein Xreps1 in Xenopus laevis Early Development

doi: 10.1371/journal.pone.0033193

Figure Lengend Snippet: (A, B) GST-XRLIP or GST alone as control were tested for their ability to pull down Xreps1(253) or Xreps1(RLIP-BD) synthesized as Myc-tagged proteins. The interaction of XRLIP with RalB G23V served as positive control. Xreps1(253) precipitated with 1 µg GST-XRLIP was analyzed. (A) The input or supernatant ranged from 1.25 µl of the 25 µl of reticulocyte extract), or (B) half an embryo from extracts of 10 embryos, were analyzed by SDS-PAGE. (C) Myc-Xreps1(253) or Myc-Xreps1(RLIP-BD) were synthesized in embryos and used to interact with endogenous XRLIP. Total lysate from 10 embryos were incubated with an anti-Myc agarose-conjugated antibody (SC-40, Santa Cruz Biotechnology) and centrifuged. The supernatant and immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-RLIP, or anti-Myc antibodies. With Myc-Xreps1(RLIP-BD), the RLIP signal was weakly detected just above an unspecific signal (<). (D) Two µg of GST-Xreps1(253), GST-Xreps1(RLIP-BD), or 1 µg of GST-XralA (G23V) preloaded using GTP-g-S, or GST alone were incubated with Myc-XRLIP (lysate from 10 embryos) and purified with glutathione-agarose beads. The precipitated proteins were resolved by SDS-PAGE, transferred onto a Hybond-P membrane (Amersham) and blotted with a monoclonal anti-Myc antibody (9E10). (E) The table recapitulates the results obtained on the in vivo and in vitro interaction of XRLIP with Xreps1.

Article Snippet: Myc-XRLIP, Myc-Xreps1(253), Myc-Xreps1(RLIP-BD), and XralB G23V proteins were synthesized using the reticulocyte extract TnT Kit (Promega) and labelled with [ 35 S] methionine (ICN) according to manufacturer's instructions.

Techniques: Control, Synthesized, Positive Control, SDS Page, Incubation, Immunoprecipitation, Purification, Membrane, In Vivo, In Vitro

(A) Animal views of gastrulae. Control uninjected embryos (a). Embryos injected in animal pole of one blastomere at the 2-cell stage with 1 ng mRNA of Myc-Xreps1(RLIP-BD) (b), Myc-Xreps1(253) (c), Mreps1 (d), or Mreps1-CAAX (e) and enlargement of hyperpigmented cell area from an embryo injected with Mreps1-CAAX (f) Red arrows show hypepigmentation area. (B) Protein expression of Xreps1(RLIP-BD) and Xreps1(253) was analyzed by Western blot with a 9E10 anti-Myc antibody, control lanes a, Xreps1(RLIP-BD) b and Xreps1(253) c, and analysis of 35 S signal from reticulocyte extracts; control d, Xreps1(RLIP-BD) e and Xreps1(253) f.

Journal: PLoS ONE

Article Title: Identification and Characterization of the RLIP/RALBP1 Interacting Protein Xreps1 in Xenopus laevis Early Development

doi: 10.1371/journal.pone.0033193

Figure Lengend Snippet: (A) Animal views of gastrulae. Control uninjected embryos (a). Embryos injected in animal pole of one blastomere at the 2-cell stage with 1 ng mRNA of Myc-Xreps1(RLIP-BD) (b), Myc-Xreps1(253) (c), Mreps1 (d), or Mreps1-CAAX (e) and enlargement of hyperpigmented cell area from an embryo injected with Mreps1-CAAX (f) Red arrows show hypepigmentation area. (B) Protein expression of Xreps1(RLIP-BD) and Xreps1(253) was analyzed by Western blot with a 9E10 anti-Myc antibody, control lanes a, Xreps1(RLIP-BD) b and Xreps1(253) c, and analysis of 35 S signal from reticulocyte extracts; control d, Xreps1(RLIP-BD) e and Xreps1(253) f.

Article Snippet: Myc-XRLIP, Myc-Xreps1(253), Myc-Xreps1(RLIP-BD), and XralB G23V proteins were synthesized using the reticulocyte extract TnT Kit (Promega) and labelled with [ 35 S] methionine (ICN) according to manufacturer's instructions.

Techniques: Control, Injection, Expressing, Western Blot