Journal: Cell Communication and Signaling : CCS

Article Title: Osteoarthritic chondrocytes undergo a glycolysis-related metabolic switch upon exposure to IL-1b or TNF

doi: 10.1186/s12964-023-01150-z

Figure Lengend Snippet: OAC and NC RNA-seq data. ( a , b ) Principal component analysis (PCA) was performed on the expression data of OAC ( a ) and NC samples ( b ). The first two eigenvalues were plotted with data ellipses for each treatment. ( c ) Summary of the number of DEGs in each condition and DEGs common between IL-1b and TNF treatments highlighted by Venn diagrams. ( d , e , f , g ) Volcano plots representing DEGs (p-adjust < 0.10; log2(Fold-Change) >|0.58|) in OACs ( d , e ) and NCs (f, g) in response to 1 ng/mL IL-1b (d, f) or 25 ng/mL TNF (e, g). ( h ) Histograms representing normalized MMP1 , SOD2 , SERPINE2 , and Slc39a8 (ZIP8) gene counts in OACs and NCs; ** P < 0.01 and *** P < 0.001, versus untreated condition using multiple t-tests with Benjamini & Hochberg correction; N = 5 per group. Values are expressed as means ± SEM

Article Snippet: After 24 h, cytokines were added as follows: 1 ng/mL IL-1b (Merck, IL038) or 25 ng/mL TNF (Miltenyi Biotec 130–094-023) or 50 ng/mL IL-6/IL-6 soluble receptor (R&D, 8954-SR-025) for 24 h. At the end of the treatments, the supernatants were collected and the cells were lysed before being stored immediately at -80 °C until further analysis.

Techniques: RNA Sequencing, Expressing

Journal: Cell Communication and Signaling : CCS

Article Title: Osteoarthritic chondrocytes undergo a glycolysis-related metabolic switch upon exposure to IL-1b or TNF

doi: 10.1186/s12964-023-01150-z

Figure Lengend Snippet: Definition of the canonical pro-inflammatory signature in OACs. ( a , b ) PCAs performed on the protein expression data from ( a ) 5 OA patients or ( b ) the 4 selected patients. The first two eigenvalues were plotted with data ellipses for each patient. ( c , d ) Volcano plots representing DEPs ( p -value < 0.05; |log2(Fold-Change)|> 0) and Venn diagram highlighting DEPs and DEGs matched in OAC in response to ( c ) 1 ng/mL IL-1b, or ( d ) 25 ng/mL TNF compared to the untreated condition. ( e , f ) KEGG enrichment analysis based on DEGs/DEPs in response to ( e ) IL-1b or ( f ) TNF. ( g ) Venn diagram highlighting 56 DEGs/DEPs common between IL-1b and TNF responses. ( h ) KEGG enrichment analysis using the list of 56 matched DEPs/DEGs

Article Snippet: After 24 h, cytokines were added as follows: 1 ng/mL IL-1b (Merck, IL038) or 25 ng/mL TNF (Miltenyi Biotec 130–094-023) or 50 ng/mL IL-6/IL-6 soluble receptor (R&D, 8954-SR-025) for 24 h. At the end of the treatments, the supernatants were collected and the cells were lysed before being stored immediately at -80 °C until further analysis.

Techniques: Expressing

Journal: Cell Communication and Signaling : CCS

Article Title: Osteoarthritic chondrocytes undergo a glycolysis-related metabolic switch upon exposure to IL-1b or TNF

doi: 10.1186/s12964-023-01150-z

Figure Lengend Snippet: Identification of a specific feature of OACs. ( a ) Venn diagram showing the 21 common DEGs among the 56 defining the pro-inflammatory signature of OACs and TNF and IL-1b responses in NCs. ( b ) Protein–protein network analysis using the pro-inflammatory signature identified in OACs ( c ) and using the 21 DEGs also found in response to IL-1b and TNF treatments in NCs. ( b , c ) Three interacting groups were revealed by this analysis: The blue circle group was characterized by common pro-inflammatory targets, the yellow circle group was associated with proteins involved in antigen presentation, and the green box group was composed of metabolism-related proteins. ( d ) RT-qPCR analysis of Glut-1, MCT-4, HK2, and GFAT2 expression in OACs and NCs after 24 h of IL-1b or TNF treatment; * P < 0.05, ** P < 0.01 using the Mann–Whitney test; N = 5 per group. Values are expressed as means ± SEM

Article Snippet: After 24 h, cytokines were added as follows: 1 ng/mL IL-1b (Merck, IL038) or 25 ng/mL TNF (Miltenyi Biotec 130–094-023) or 50 ng/mL IL-6/IL-6 soluble receptor (R&D, 8954-SR-025) for 24 h. At the end of the treatments, the supernatants were collected and the cells were lysed before being stored immediately at -80 °C until further analysis.

Techniques: Immunopeptidomics, Quantitative RT-PCR, Expressing, MANN-WHITNEY