Journal: RNA (New York, N.Y.)

Article Title: Small molecule telomerase inhibitors are also potent inhibitors of telomeric C-strand synthesis.

doi: 10.1261/rna.080043.124

Figure Lengend Snippet: FIGURE 1. Inhibition of CST–PP activity by TMPyP4. (A) Representative gels showing C-strand synthesis on the 9xTEL template in NaCl/KCl (left) or LiCl (right) with trace amount of radiolabeled template (T) serving as recovery control. Twofold dilutions of TMPyP4 (highest concentration: 3200 nM) were incubated with the template before the addition of CST–PP and nucleotides. (B) Plot of relative synthesis intensities as a function of TMPyP4 concentration in NaCl/KCl or LiCl. Points represent mean values and error bars represent SD for n = 5 (Na+/K+) or n = 4 (Li+) indepen- dent experiments. (C,D) Same as panels A,B except that the template was 9xnoGG. For panel D, n = 4.

Article Snippet: TMPyP4 (sc-204346, Santa Cruz Biotechnology) and PDS (SML2690, Sigma-Aldrich) were resuspended in 100% DMSO at 10 mg/mL and stored at −20°C.

Techniques: Inhibition, Activity Assay, Control, Concentration Assay, Incubation

Journal: RNA (New York, N.Y.)

Article Title: Small molecule telomerase inhibitors are also potent inhibitors of telomeric C-strand synthesis.

doi: 10.1261/rna.080043.124

Figure Lengend Snippet: FIGURE 2. TMPyP4 inhibits CST–PP binding to template DNA. (A) Representative gels of CST–PP binding to radiolabeled template DNA in NaCl/KCl (left) or LiCl (right) following incubation of DNA with increasing concentrations of TMPyP4 (twofold dilutions from 3200 nM). (T) Template, (T–P) template–protein complex, (W) well. (B) Plot of fraction DNA bound at each concentration of TMPyP4 normalized to fraction bound with no inhibitor in NaCl/KCl or LiCl. Points represent mean values and error bars represent SD for n = 6 independent experiments, except 9xnoGG in Li+ (n = 4).

Article Snippet: TMPyP4 (sc-204346, Santa Cruz Biotechnology) and PDS (SML2690, Sigma-Aldrich) were resuspended in 100% DMSO at 10 mg/mL and stored at −20°C.

Techniques: Binding Assay, Incubation, Concentration Assay

Journal: RNA (New York, N.Y.)

Article Title: Small molecule telomerase inhibitors are also potent inhibitors of telomeric C-strand synthesis.

doi: 10.1261/rna.080043.124

Figure Lengend Snippet: FIGURE 4. (A) Native gel electrophoresis with 9xTEL DNA incubated with twofold dilutions of TMPyP4 (highest concentration: 3200 nM) in NaCl/KCl (left half of gel) or LiCl (right half). (B) As in panel A but with 9xnoGG DNA. (C) Native gel of 9xTEL DNA incubated with twofold dilutions of PDS (highest concentration: 1600 nM) in NaCl/KCl (left half) or LiCl (right half). (D) As in panel C but with 9xnoGG DNA.

Article Snippet: TMPyP4 (sc-204346, Santa Cruz Biotechnology) and PDS (SML2690, Sigma-Aldrich) were resuspended in 100% DMSO at 10 mg/mL and stored at −20°C.

Techniques: Nucleic Acid Electrophoresis, Incubation, Concentration Assay

Journal: Journal of Biomedical Science

Article Title: Targeting the G-quadruplex as a novel strategy for developing antibiotics against hypervirulent drug-resistant Staphylococcus aureus

doi: 10.1186/s12929-024-01109-3

Figure Lengend Snippet: Confirmation of antibacterial activity. A The antibacterial activities of NMM, TMPyP2, BRACO19, TMPyP4, and Thioflavin T against SAUSA300 were examined by measuring cell growth in terms of OD at 600 nm. B, C SAUSA300 cell growth assessed as CFU/mL ( B ); representative sheep-blood agar plates showing the appearance of colonies during CFU enumeration ( C ). D , E Comparative killing kinetics of vancomycin (Van), Tetracycline (Tet), and NMM against SAUSA300 based on CFU/mL at 1.0 × ( D ); and 10 × ( E ) MIC of Van, Tet, NMM, and 0.05% Triton X-100 at different time points (0 to 12 h). F Comparative live/dead assay of SAUSA300 with 1 × MIC of NMM (5 µM) and Van (0.6 µM) using confocal microscopy, showing the proportion of live/dead SAUSA300 cells. SYTO9 and PI were used to stain the number of total and dead cells as green-fluorescent and red-fluorescent cells, respectively. All experiments were performed in triplicate and the average data was plotted with standard deviation. Significance of the data was analyzed using Student’s t -test. p -values less than 0.05 were considered significant (ns = non-significant p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.0001)

Article Snippet: The G4 compounds were procured as BRACO19 trihydrochloride (GC50140, GLPBIO); Quarfloxin or CX-3543 (A12380, AdooQ), TMPyP2 or meso-Tetra (3-pyridyl) porphine (T40846, Frontier Scientific), TMPyP4 tosylate (GC12092, GLPBIO), PDS or Pyridostatin trifluoroacetate salt (18013, Cayman Chemical), PhenDC3 trifluoromethanesulfonate (CS-7711, Chemscene), Thioflavin T (2390-54-7, MedChemExpress), Quinacrine dihydrochloride (69-05-6, MedChemExpress), N-methyl mesoporphyrin IX or NMM (GC44416, GLPBIO), and Quercetin (117-39-5, MedChemExpress) (Fig. S1).

Techniques: Activity Assay, Live Dead Assay, Confocal Microscopy, Staining, Standard Deviation

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

Article Title: Utilisation of Targeted Nanoparticle Photosensitiser Drug Delivery Systems for the Enhancement of Photodynamic Therapy

doi: 10.3390/molecules23102628

Figure Lengend Snippet: Targeting moiety approaches used to enhance NP PSs active drug delivery systems in tumours.

Article Snippet: DNA G-quadruplex Aptamer , Direct , Sgc8 leukemia aptamer, which can specifically bind to protein tyrosine kinase 7 (PTK7) receptor , CEM cells CEM (CCL-119, T-cell line, human & Ramos (CRL-1596, B-cell line, human Burkitt’s lymphoma) & Cervical cancer (HeLa) mouse models , In vitro & in vivo , 5, 10, 15, 20-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP4) , Zr-based nanoscale metal-organic frameworks (Zr-NMOFs) , Nanosystem induced 90% cell death of targeted cells & maintained more than 76% tumour inhibition within the entire experimental period. , [ ] .

Techniques: Over Expression, In Vitro, In Vivo, Inhibition, Irradiation

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: The structure of the free-base ligand (H 2 TMPyP 4+ = 5,10,15,20-tetrakis(1-methyl-4-pyridinium-)porphyrin).

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques:

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: Soret bands of Ni(II)TMPyP 4+ in various media.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques:

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: ( a ) Molar absorptivity of Q-bands and S1 fluorescence of the high-spin Ni(II)TMPyP 4+ complex. ( b ) Molar absorptivity of Q-bands and S 1 fluorescence of the low-spin Ni(II)TMPyP 4+ complex.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques: Fluorescence

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: Excitation and absorption spectra of Ni(II)TMPyP 4+ in water, an equilibrium system involving both high-spin and low-spin species.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques:

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: Change in absorption spectra of Ni(II)TMPyP 4+ with increasing temperature in aqueous solution. The red spectrum belongs to 20 °C, the blue one to 75 °C.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques:

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: Thermodynamic data calculated for the coordination and spin-state equilibrium of Ni(II)TMPyP 4+ .

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques:

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: Spectral change in the Ni(II)TMPyP 4+ solution upon 455 nm irradiation. The blue spectrum was recorded at the end of irradiation.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques: Irradiation

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: Concentration changes in the low- and high-spin forms during the 455 nm irradiation of the Ni(II)TMPyP 4+ solution.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques: Concentration Assay, Irradiation

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: Spectral changes in the Soret band region for the Ni(II)TMPyP 4+ solution with increasing TEOA concentration in the range of 0–0.5 M. The blue spectrum belongs to 0 M, the red one to 0.5 M.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques: Concentration Assay

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: Partial mole fractions of different Ni(II)TMPyP 4+ species as functions of the TEOA concentration.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques: Concentration Assay

Journal: Molecules

Article Title: Effects of Temperature, Axial Ligand, and Photoexcitation on the Structure and Spin-State of Nickel(II) Complexes with Water-Soluble 5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin

doi: 10.3390/molecules29020310

Figure Lengend Snippet: The slope of the line obtained as a function of the TEOA concentration per Equation (5) yields the equilibrium constant regarding the association of Ni(II)TMPyP 4+ with TEOA.

Article Snippet: Tetrachloride salt of Ni(II)TMPyP 4+ was purchased from Frontier Scientific (Newark, DE, USA).

Techniques: Concentration Assay