Journal: Journal of Veterinary Science

Article Title: Toll-like receptor 3-stimulation and aggregate-formation synergistically enhances anti-inflammatory activity of feline mesenchymal stem cells

doi: 10.4142/jvs.23330

Figure Lengend Snippet: IDO-1, indoleamine 2,3-dioxygenase-1; bFGF, basic fibroblast growth factor; TLR3, toll-like receptor 3; fAdMSC, feline adipose-derived mesenchymal stem cell. * p < 0.05.

Article Snippet: TLR3 was stimulated by suspending fAdMSCs were suspended in α-MEM (Nacalai Tesque, Japan) containing 15% fetal bovine serum (HyClone, GE Healthcare UK Ltd., England), penicillin and streptomycin (100 units/mL and 100 μg/mL, respectively, FUJIFILM Wako Pure Chemical Corporation, Japan) (hereafter, culture medium), seeding in wells of a six-well plate (AGC Techno Glass, Japan), and incubating them with a TLR3 ligand (poly [I:C], 1 μg/mL, FUJIFILM Wako Pure Chemical Corporation) for 1 h at 37°C in 5% CO 2 and 95% air.

Techniques: Derivative Assay

Journal: Journal of Veterinary Science

Article Title: Toll-like receptor 3-stimulation and aggregate-formation synergistically enhances anti-inflammatory activity of feline mesenchymal stem cells

doi: 10.4142/jvs.23330

Figure Lengend Snippet: IDO-1, indoleamine 2,3-dioxygenase-1; TLR3, toll-like receptor 3; fAdMSC, feline adipose-derived mesenchymal stem cell.

Article Snippet: TLR3 was stimulated by suspending fAdMSCs were suspended in α-MEM (Nacalai Tesque, Japan) containing 15% fetal bovine serum (HyClone, GE Healthcare UK Ltd., England), penicillin and streptomycin (100 units/mL and 100 μg/mL, respectively, FUJIFILM Wako Pure Chemical Corporation, Japan) (hereafter, culture medium), seeding in wells of a six-well plate (AGC Techno Glass, Japan), and incubating them with a TLR3 ligand (poly [I:C], 1 μg/mL, FUJIFILM Wako Pure Chemical Corporation) for 1 h at 37°C in 5% CO 2 and 95% air.

Techniques: Derivative Assay

Journal: Journal of Veterinary Science

Article Title: Toll-like receptor 3-stimulation and aggregate-formation synergistically enhances anti-inflammatory activity of feline mesenchymal stem cells

doi: 10.4142/jvs.23330

Figure Lengend Snippet: IDO-1, indoleamine 2,3-dioxygenase-1; bFGF, basic fibroblast growth factor; TLR3, toll-like receptor 3; fAdMSC, feline adipose-derived mesenchymal stem cell. * p < 0.05.

Article Snippet: TLR3 was stimulated by suspending fAdMSCs were suspended in α-MEM (Nacalai Tesque, Japan) containing 15% fetal bovine serum (HyClone, GE Healthcare UK Ltd., England), penicillin and streptomycin (100 units/mL and 100 μg/mL, respectively, FUJIFILM Wako Pure Chemical Corporation, Japan) (hereafter, culture medium), seeding in wells of a six-well plate (AGC Techno Glass, Japan), and incubating them with a TLR3 ligand (poly [I:C], 1 μg/mL, FUJIFILM Wako Pure Chemical Corporation) for 1 h at 37°C in 5% CO 2 and 95% air.

Techniques: Derivative Assay

Journal: Journal of Veterinary Science

Article Title: Toll-like receptor 3-stimulation and aggregate-formation synergistically enhances anti-inflammatory activity of feline mesenchymal stem cells

doi: 10.4142/jvs.23330

Figure Lengend Snippet: IDO-1, indoleamine 2,3-dioxygenase-1; bFGF, basic fibroblast growth factor; COX-2, cyclooxygenase-2; TNF-α, tumor necrosis factor-α; LPS, lipopolysaccharide; TLR3, toll-like receptor 3; fAdMSC, feline adipose-derived mesenchymal stem cell. * p < 0.05.

Article Snippet: TLR3 was stimulated by suspending fAdMSCs were suspended in α-MEM (Nacalai Tesque, Japan) containing 15% fetal bovine serum (HyClone, GE Healthcare UK Ltd., England), penicillin and streptomycin (100 units/mL and 100 μg/mL, respectively, FUJIFILM Wako Pure Chemical Corporation, Japan) (hereafter, culture medium), seeding in wells of a six-well plate (AGC Techno Glass, Japan), and incubating them with a TLR3 ligand (poly [I:C], 1 μg/mL, FUJIFILM Wako Pure Chemical Corporation) for 1 h at 37°C in 5% CO 2 and 95% air.

Techniques: Derivative Assay