Journal: Cell Research

Article Title: Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines

doi: 10.1038/s41422-021-00514-9

Figure Lengend Snippet: a NT 50 values for COVID-19 convalescent plasma (CE) and CoronaVac vaccinee plasma (I) using D614G mutant pseudovirus neutralization assay. Statistical significance was determined using one-tailed t -test ( **P < 0.01). b Plasma anti-IgG ELISA reactivity to S(ECD), RBD, and NTD. Normalized area under the curve (AUC) values are plotted. Black dots stand for CoronaVac vaccinees, and red dots stand for convalescents. c Comparison of anti-IgG ELISA AUC values among RBD.V2 (triangle), D614G (circle), and 242–244Δ (diamond) mutants of S1. White stands for CoronaVac vaccinees, and red stands for convalescents. Statistical significance was determined using paired one-tailed t -test ( ***P < 0.001). d NT 50 fold-change from D614G to 501Y.V2 for convalescent and CoronaVac vaccinee plasma by pseudovirus neutralization assay. Statistical significance was determined using one-tailed t -test (n.s., no significance). e NT 50 fold-change from WT to 501Y.V2 for convalescent and CoronaVac vaccinee plasma by authentic virus neutralization assay. Statistical significance was determined using one-tailed t -test (n.s., no significance). f Comparison of NT 50 values by pseudovirus or authentic virus neutralization assay between WT/D614G and the indicated mutants for CoronaVac vaccinee plasma samples. g Comparison of NT 50 values by pseudovirus or authentic virus neutralization assay between WT/D614G and the indicated mutants for CE samples. h Summary of the fold change of NT 50 for the indicated mutants from D614G. Color gradient indicates fold change values ranging from +1 (white) to −6.7 (blue). All plotted values and horizontal bars in this figure indicate the geometric mean.

Article Snippet: Plasma IgG responses to SARS-CoV-2 S (SARS-CoV-2 Spike S1 + S2 ECD Antibody Titer Assay Kit, Sino Biological Inc., KIT 004), RBD (SARS-CoV-2 Spike RBD Antibody Titer Assay Kit, Sino Biological Inc., KIT 002), NTD (SARS-CoV-2 Spike S1 NTD-His & AVI Recombinant Protein, Sino Biological Inc., 0591-V49H), S1-D614G (SARS-CoV-2 Spike S1(D614G)-His Recombinant Protein, Sino Biological Inc., 40591-V08H3), S1-RBD.V2 (SARS-CoV-2 Spike S1(K417N, E484K, N501Y, D614G)-His Recombinant Protein, Sino Biological Inc., 40591-V08H10), S1–501Y.V2 NTD (SARS-CoV-2 Spike S1(242–244Δ, D614G)-His, Sino Biological Inc., BD61–2) were measured by ELISA.

Techniques: Clinical Proteomics, Mutagenesis, Neutralization, One-tailed Test, Enzyme-linked Immunosorbent Assay, Comparison, Virus

Journal: Cell Research

Article Title: Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines

doi: 10.1038/s41422-021-00514-9

Figure Lengend Snippet: a NT 50 values for ZF2001 vaccinee plasma/sera using D614G mutant pseudovirus neutralization assay. Statistical significance was determined using one-tailed t -test (* P < 0.05; n.s., no significance). b Plasma/serum anti-IgG ELISA reactivity to S(ECD), RBD, and NTD. Normalized AUC values are plotted. Black circles stand for R1–R10, and red circles stand for R11–R20. c Comparison of anti-IgG ELISA AUC values among RBD.V2 (triangle), D614G (circle), and 242–244Δ (diamond) mutants of S1. White stands for R1–R10, and red stands for R11–R20. Statistical significance was determined using paired one-tailed t -test (*** P < 0.001; n.s., no significance). d NT 50 fold-change from D614G to 501Y.V2 for ZF2001 vaccinee plasma/sera by pseudovirus neutralization assay. Statistical significance was determined using one-tailed t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). e NT 50 fold-change from WT to 501Y.V2 for ZF2001 vaccinee plasma/sera by authentic virus neutralization assay. Statistical significance was determined using one-tailed t -test (* P < 0.05; n.s., no significance). f Comparison of NT 50 values by pseudovirus or authentic virus neutralization assay between WT/D614G and the indicated mutants for ZF2001 vaccinee plasma samples. g Summary of the fold change of NT 50 for the indicated mutants from D614G. Color gradient indicates fold change values ranging from +1 (white) to −6.1 (blue). All plotted values and horizontal bars in this figure indicate the geometric mean. h Pie charts indicating the distribution of antibody sequences from two ZF2001 vaccinees. The number in the inner circle indicates the number of single cells analyzed. Slice size is proportional to the number of clonal enrichment frequencies. The color of the slice indicates neutralization potency toward pseudovirus mutants. Gray, expressed antibodies but with low or no neutralization (IC 50 > 1 μg/mL) and darker gray indicates lower potency; red, high neutralization potency (IC 50 < 1 μg/mL) and darker red indicates higher potency; white, not expressed.

Article Snippet: Plasma IgG responses to SARS-CoV-2 S (SARS-CoV-2 Spike S1 + S2 ECD Antibody Titer Assay Kit, Sino Biological Inc., KIT 004), RBD (SARS-CoV-2 Spike RBD Antibody Titer Assay Kit, Sino Biological Inc., KIT 002), NTD (SARS-CoV-2 Spike S1 NTD-His & AVI Recombinant Protein, Sino Biological Inc., 0591-V49H), S1-D614G (SARS-CoV-2 Spike S1(D614G)-His Recombinant Protein, Sino Biological Inc., 40591-V08H3), S1-RBD.V2 (SARS-CoV-2 Spike S1(K417N, E484K, N501Y, D614G)-His Recombinant Protein, Sino Biological Inc., 40591-V08H10), S1–501Y.V2 NTD (SARS-CoV-2 Spike S1(242–244Δ, D614G)-His, Sino Biological Inc., BD61–2) were measured by ELISA.

Techniques: Clinical Proteomics, Mutagenesis, Neutralization, One-tailed Test, Enzyme-linked Immunosorbent Assay, Comparison, Virus