Journal:

Article Title: pp32 Reduction Induces Differentiation of TSU-Pr1 Cells

doi:

Figure Lengend Snippet: Expression of IL-6 in TSU-Pr1 cells. TSU-Pr1 cells stably transfected with pp32 anti-sense express higher levels of IL-6 message as compared to parental TSU-Pr1 cells and vector-only control by RT-PCR analysis, which validates the cDNA microarray analysis (see Figure 6).

Article Snippet: Microarray Analysis of TSU-Pr1 Cell Lines This procedure was performed at The Johns Hopkins University Oncology Microarray facility by using a human glass 12K cDNA chip.

Techniques: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Control, Reverse Transcription Polymerase Chain Reaction, Microarray

Journal: Stem cell research & therapy

Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.

doi: 10.1186/s13287-020-01711-w

Figure Lengend Snippet: Fig. 1 The TRF3 expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values

Article Snippet: Reintroduction of TRF3 into TRF3−/− hESCs The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCST2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDHEF1-3×Flag-TRF3-T2A-Puro.

Techniques: Expressing, Microscopy, Microarray, Quantitative RT-PCR

Journal: Stem cell research & therapy

Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.

doi: 10.1186/s13287-020-01711-w

Figure Lengend Snippet: Fig. 3 The expression levels of pluripotent markers are comparable among the undifferentiated TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a qRT- PCR analysis of pluripotency markers OCT4 and NANOG. n = 3. b Flow cytometry analysis of OCT4 and SSEA4. c Immunocytochemical staining analysis of OCT4 and SSEA4. Similar results were obtained from three independent experiments. Scale bar = 50 μm. d Flow cytometry analysis of cell cycle in undifferentiated hESCs. n = 3. e Representative analysis of clones positive for alkaline phosphatase staining. Similar results were obtained from three independent experiments. Scale bar = 500 μm. Data are presented as mean ± SEM

Article Snippet: Reintroduction of TRF3 into TRF3−/− hESCs The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCST2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDHEF1-3×Flag-TRF3-T2A-Puro.

Techniques: Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Clone Assay

Journal: Cancer cell

Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma

doi: 10.1016/j.ccell.2017.04.002

Figure Lengend Snippet: (A) GNAQQ209L transfected alone or combined with PKC δ or ε into 293FT cells increased Ras-GTP levels. 293FT cells were transfected with indicated cDNA plasmids for 24 hr. Ras-GTP pull-down was performed and detected by western blot with a pan-Ras antibody. TPA stimulation was used a positive control. Expression levels of GNAQQ209L were monitored with Glu-Glu (EE) tag. PKC δ and ε were detected via HA tags.

Article Snippet: cDNA: HA-PKC δ(kinase dead) , addgene , 16389.

Techniques: Transfection, Western Blot, Positive Control, Expressing

Journal: Cancer cell

Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma

doi: 10.1016/j.ccell.2017.04.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: cDNA: HA-PKC δ(kinase dead) , addgene , 16389.

Techniques: Recombinant, Activation Assay, Microarray, Fractionation, Expressing, Cell Culture

Journal: Cancer cell

Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma

doi: 10.1016/j.ccell.2017.04.002

Figure Lengend Snippet: (A) GNAQQ209L transfected alone or combined with PKC δ or ε into 293FT cells increased Ras-GTP levels. 293FT cells were transfected with indicated cDNA plasmids for 24 hr. Ras-GTP pull-down was performed and detected by western blot with a pan-Ras antibody. TPA stimulation was used a positive control. Expression levels of GNAQQ209L were monitored with Glu-Glu (EE) tag. PKC δ and ε were detected via HA tags.

Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

Techniques: Transfection, Western Blot, Positive Control, Expressing

Journal: Cancer cell

Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma

doi: 10.1016/j.ccell.2017.04.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

Techniques: Recombinant, Activation Assay, Microarray, Fractionation, Expressing, Cell Culture

Journal: Nutrients

Article Title: Long-Term Consumption of a Sugar-Sweetened Soft Drink in Combination with a Western-Type Diet Is Associated with Morphological and Molecular Changes of Taste Markers Independent of Body Weight Development in Mice

doi: 10.3390/nu14030594

Figure Lengend Snippet: Transcriptome analysis of 59 selected genes associated with oral chemosensation, displayed as a heatmap showing mean fold changes in gene expression of the SSB-fed diet groups in relation to the respective water-fed group (=1) in the form of a color code. The gene expression was analyzed using one customized cDNA microarray per group from pooled RNA samples of the CV from mice that received either a standard diet (chow, n = 10–11) or Western-type diet (WD, n = 7–8) with water (Water) or a sugar-sweetened beverage (SSB) as a drink for 24 weeks.

Article Snippet: The isolated RNA samples per mouse were reverse transcribed to cDNA using the LunaScript RT Supermix Kit (New England Biolabs GmbH, Frankfurt am Main, Germany).

Techniques: Expressing, Microarray, Western Blot

Journal: Journal of Cellular Physiology

Article Title: ELF4/TRIB3/CDK6 Axis Promotes Cancer Stem Cell Activity in Endometrial Cancer

doi: 10.1002/jcp.70113

Figure Lengend Snippet: ELF4 is highly expressed in EC and is associated with poor prognosis. (A) mRNA expression of ELF4 in endometrioid tumors, papillary serous cancers, and normal endometrial tissues from GEO dataset GSE17025 . Data presented as mean ± SD. The p values were adjusted by Tukey's method between each group. (B) Spearman correlation analysis of ELF4 expression and histological grade in TCGA Uterine Corpus Endometrial Carcinoma (UCEC) database, analyzed using TISIDB. (C) IHC analysis of ELF4 protein expression across histological grades in slides of an EC tissue microarray. Left panel: Representative images of ELF4 staining intensity in EC specimens, with insets showing magnified view of indicated area. Right panel: Quantification of ELF4 expression in human EC specimens using HistoQuest software, presented as mean ± SD; * p < 0.05 by Tukey's multiple comparisons test. (D, E) Kaplan‐Meier curves of UCEC patients from TCGA database, stratified by median ELF4 mRNA expression levels: (D) Overall survival (OS) and (E) Recurrence‐free survival (RFS). Significance determined by log‐rank test for both.

Article Snippet: The human ELF4 cDNA (RefSeq NM_001421 ) tagged with Myc‐DDK was obtained from OriGene Technologies (Cat. No. RC200479, Rockville, MD, USA).

Techniques: Expressing, Microarray, Staining, Software

Journal: Journal of Cellular Physiology

Article Title: ELF4/TRIB3/CDK6 Axis Promotes Cancer Stem Cell Activity in Endometrial Cancer

doi: 10.1002/jcp.70113

Figure Lengend Snippet: Knockdown of ELF4 suppresses cell proliferation and reduces CDK6 protein levels in EC cells. (A–C) Colony formation assay in (A) AN3CA, (B) HEC‐1A, and (C) EMC6 cells transduced with shLacZ or ELF4‐specific short hairpin RNAs (shRNAs), followed by crystal violet staining and colony counting. (D) Cell cycle analysis by propidium iodide (PI) staining and flow cytometry in cells transduced with shLacZ or ELF4‐specific shRNAs. Data are presented as mean ± SD; * p < 0.05; ** p < 0.01; *** p < 0.001 versus shLacZ‐transduced cells, unpaired Student's t test. (E–G) Western blot analysis of ELF4 and CDK6 protein levels in (E) AN3CA, (F) HEC‐1A, and (G) EMC6 cells transduced with shLacZ or ELF4‐specific shRNAs, using GAPDH as loading control. Band intensities were quantified and expressed as mean ± SEM; *** p < 0.001 versus shLacZ, unpaired Student's t test.

Article Snippet: The human ELF4 cDNA (RefSeq NM_001421 ) tagged with Myc‐DDK was obtained from OriGene Technologies (Cat. No. RC200479, Rockville, MD, USA).

Techniques: Knockdown, Colony Assay, Transduction, Staining, Cell Cycle Assay, Flow Cytometry, Western Blot, Control

Journal: Journal of Cellular Physiology

Article Title: ELF4/TRIB3/CDK6 Axis Promotes Cancer Stem Cell Activity in Endometrial Cancer

doi: 10.1002/jcp.70113

Figure Lengend Snippet: ELF4 transcriptionally regulates CDK6 expression in EC cells. (A) Spearman's correlation analysis of ELF4 and CDK6 expression in UCEC patients, performed using GEPIA2. (B) Relative mRNA levels of ELF4 and CDK6 in AN3CA, HEC‐1A, and EMC6 cells transfected with control (siCtrl) or ELF4‐specific small interfering RNAs (siELF4), measured by SYBR‐Green‐based quantitative reverse transcription PCR (qRT‐PCR). Data are fold changes (mean ± SD) versus siCtrl; * p < 0.05; ** p < 0.01; *** p < 0.001, unpaired Student's t ‐test. (C, D) Western blot analysis of CDK6, ELF4, and GAPDH expression in AN3CA, HEC‐1A, and EMC6 cells transfected with siCtrl or siELF4, using GAPDH as loading control. Band intensities quantified by ImageJ were presented as mean ± SEM (D); ** p < 0.01; *** p < 0.001 versus siCtrl, Student's t test. (E) Upper panel: CDK6 promoter with putative ELF4 binding motifs (red bars). Lower panel: Luciferase reporter assay in AN3CA, HEC‐1A, and EMC6 cells co‐transfected with pGL3‐CDK6 promoter constructs and pCMV‐NCV or ELF4‐Flag plasmids. Data were presented as fold changes (mean ± SD) versus pCMV‐NCV; ** p < 0.01, unpaired Student's t ‐test. (F) Chromatin immunoprecipitation (ChIP) assay in AN3CA and HEC‐1A cells using anti‐ELF4 antibody or IgG control, followed by qPCR detection of the ELF4 binding fragments within CDK6 promoter. Data presented as mean ± SD; * p < 0.05 versus anti‐IgG, unpaired Student's t ‐test. Western blot analysis of CDK6, ELF4‐Flag, and GAPDH expression in 293 T cells (G) or HEC‐1A cells (H) transfected with pCMV‐NCV or ELF4‐Flag vectors, using GAPDH as loading control. Band intensities quantified by ImageJ are mean ± SEM. ** p < 0.01 versus pCMV‐NCV, unpaired Student's t test (G). * p < 0.05 versus pLVX‐puro, unpaired Student's t ‐test (H).

Article Snippet: The human ELF4 cDNA (RefSeq NM_001421 ) tagged with Myc‐DDK was obtained from OriGene Technologies (Cat. No. RC200479, Rockville, MD, USA).

Techniques: Expressing, Transfection, Control, SYBR Green Assay, Reverse Transcription, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Reporter Assay, Construct, Chromatin Immunoprecipitation

Journal: Journal of Cellular Physiology

Article Title: ELF4/TRIB3/CDK6 Axis Promotes Cancer Stem Cell Activity in Endometrial Cancer

doi: 10.1002/jcp.70113

Figure Lengend Snippet: Knockdown of ELF4 reduces β‐catenin and c‐MYC protein levels and inhibits tumorsphere formation. Western blot analysis of β‐catenin, c‐MYC, and GAPDH expression in (A) AN3CA, (B) HEC‐1A, and (C) EMC6 cells transduced with shLacZ or ELF4‐specific short hairpin RNAs (shELF#1 or shELF4#2), using GAPDH as loading control. Band intensities are mean ± SEM; *** p < 0.001 versus shLacZ, unpaired Student's t test. Tumorsphere formation assay in cells transduced with shLacZ or ELF4‐specific shRNAs (shELF#1 or shELF4#2), assessed by (D) imaging and (E) counting tumorspheres after 7‐10 days using an inverted light microscope. Data are presented as mean ± SD; * p < 0.05; ** p < 0.01; *** p < 0.001 versus shLacZ‐transduced cells, unpaired Student's t ‐test.

Article Snippet: The human ELF4 cDNA (RefSeq NM_001421 ) tagged with Myc‐DDK was obtained from OriGene Technologies (Cat. No. RC200479, Rockville, MD, USA).

Techniques: Knockdown, Western Blot, Expressing, Transduction, Control, Tube Formation Assay, Imaging, Light Microscopy

Journal: Journal of Cellular Physiology

Article Title: ELF4/TRIB3/CDK6 Axis Promotes Cancer Stem Cell Activity in Endometrial Cancer

doi: 10.1002/jcp.70113

Figure Lengend Snippet: Inhibition of CDK6 blocks self‐renewal activity in EC. (A) MTT assay of cell viability in AN3CA, HEC‐1A, and EMC6 cells transfected with control (siCtrl) or CDK6‐specific small interfering RNAs (siCDK6), plated at 3000 cells/well in 96‐well plates. Data are mean ± SD; * p < 0.05; *** p < 0.001 versus siCtrl, unpaired Student's t ‐test. Tumorsphere formation assay in AN3CA, HEC‐1A, and EMC6 cells transfected with siCtrl or siCDK6, assessed by (B) imaging and (C) counting tumorspheres after 7–10 days using an inverted light microscope. Data are presented as mean ± SD; * p < 0.05; *** p < 0.001 versus siCtrl, unpaired Student's t ‐test. (D) Western blot analysis of CDK6, OCT4, NANOG, c‐MYC, and GAPDH expression in AN3CA, HEC‐1A, and EMC6 cells transfected with siCtrl or siCDK6, using GAPDH as loading control. Relative band intensities versus siCtrl were indicated. (E) MTT assay of cell viability in AN3CA, HEC‐1A, EMC4, EMC5, and EMC6 cells seeded at 3000 cells/well in 96‐well plates and treated with increasing Palbociclib doses. Data are presented as relative viability (mean ± SD) versus vehicle controls. Tumorsphere formation assay in AN3CA, HEC‐1A, and EMC6 cells seeded at 1 × 10⁴ cells/well in ultra‐low attachment 6‐well plates, treated with Palbociclib or vehicle for 7–10 days, assessed by (F) imaging and (G) counting tumorspheres using an inverted light microscope. Scale bar: 100 μm. Data presented as are mean ± SD; *** p < 0.001 versus vehicle, unpaired Student's t ‐test. (H, I) Western blot analysis of NANOG, OCT4, c‐MYC, and GAPDH expression in AN3CA, HEC‐1A, and EMC6 cells treated with Palbociclib or vehicle, using GAPDH as loading control. Relative band intensities versus vehicle from three independent repeats were shown in (I). * p < 0.05; ** p < 0.01; *** p < 0.001 vs vehicle control (Ctrl), unpaired Student's t ‐test. (J, K, L) HEC‐1A cells were transduced with lentiviruses carrying shLacZ or shELF4 sequences followed by transfecting with vectors of empty (NCV) or myc‐tagged CDK6 cDNA (CDK6‐myc). The expressions of myc‐tagged CDK6 and ELF4 were confirmed by western blot (J). Cell viability at 72 h or 96 h post‐transduction was determined by MTT (K). ** p < 0.01; *** p < 0.001; # p < 0.05; ### p < 0.001. CSC activity was examined by tumorsphere assay (L).

Article Snippet: The human ELF4 cDNA (RefSeq NM_001421 ) tagged with Myc‐DDK was obtained from OriGene Technologies (Cat. No. RC200479, Rockville, MD, USA).

Techniques: Inhibition, Activity Assay, MTT Assay, Transfection, Control, Tube Formation Assay, Imaging, Light Microscopy, Western Blot, Expressing, Transduction

Journal: Journal of Cellular Physiology

Article Title: ELF4/TRIB3/CDK6 Axis Promotes Cancer Stem Cell Activity in Endometrial Cancer

doi: 10.1002/jcp.70113

Figure Lengend Snippet: TRIB3 cooperates with ELF4 to regulate CDK6 expression in EC. (A) Heatmap of CDK6 expression in TRIB3‐knocked‐down HEC‐1A cells, generated using ClustVis from two independent replicates. (B) Spearman's correlation analysis of TRIB3 and CDK6 expression in UCEC patients of TCGA database, performed using GEPIA2. (C) Relative mRNA levels of TRIB3 and CDK6 in AN3CA, HEC‐1A, and EMC6 cells transduced with shLacZ or TRIB3‐specific short hairpin RNAs (shTRIB3#1 or shTRIB3#2), measured by SYBR‐Green‐based qRT‐PCR. Data are presented as fold changes (mean ± SD) versus shLacZ; ** p < 0.01; *** p < 0.001. (D) Western blot analysis of CDK6, TRIB3, and GAPDH expression in AN3CA, HEC‐1A, and EMC6 cells transduced with shLacZ or TRIB3‐specific shRNAs, using GAPDH as loading control. Relative band intensities versus shLacZ were indicated. (E) Spearman's correlation analysis of TRIB3/ELF4 and CDK6 expression in UCEC patients of TCGA database, performed using GEPIA2. (F) ChIP‐qPCR assay assessing TRIB3 binding to putative ELF4 binding elements on the CDK6 promoter in AN3CA and HEC‐1A cells, using anti‐TRIB3 antibody or normal rabbit IgG as control. Data are presented as mean ± SD; * p < 0.05; ** p < 0.01, *** p < 0.001 versus anti‐IgG, unpaired Student's t ‐test. (G) 293 T cells were transfected with the indicated plasmids for 48 h and total cell lysates were extracted as input. After IP with anti‐HA or control mouse IgG (mIG) antibodies, the pull down proteins were subjected to western blot for HA tagged TRIB3 fragments or flag tagged ELF4. (H, I) 293 T cells were transfected with indicated plasmids for 48 h and total cell lysates were extracted and subjected to western blot for analyzing the levels of flag tagged ELF4, HA tagged TRIB3 fragments, and CDK6 (H). GAPDH was used as a protein loading control. The quantitative data of band intensities were quantified by ImageJ three independent experiment repeats are presented as mean ± SEM (I). ** p < 0.01; *** p < 0.001; # p < 0.05; ## p < 0.01 by unpaired Student's t ‐test. n.s., not significant.

Article Snippet: The human ELF4 cDNA (RefSeq NM_001421 ) tagged with Myc‐DDK was obtained from OriGene Technologies (Cat. No. RC200479, Rockville, MD, USA).

Techniques: Expressing, Generated, Transduction, SYBR Green Assay, Quantitative RT-PCR, Western Blot, Control, ChIP-qPCR, Binding Assay, Transfection

Journal: Journal of Cellular Physiology

Article Title: ELF4/TRIB3/CDK6 Axis Promotes Cancer Stem Cell Activity in Endometrial Cancer

doi: 10.1002/jcp.70113

Figure Lengend Snippet: Combination of CDK6 and β‐catenin inhibition enhances EC‐CSC targeting effects. (A) Cells were treated with palbociclib and/or CCT031374 (CCT) as indicated. After 72 h, cell viability was measured by MTT assay. ** p < 0.01; *** p < 0.001, compared to cells without CCT treatment. ### p < 0.001. Cells were treated with 5 μM palbociclib, 5 μM CCT, or a combination of both drugs at 5 μM, followed by tumorsphere cultivation. Tumorspheres were imaged and counted using inverted microscopy on Day 7 (B); quantification data are shown in (C). * p < 0.05; ** p < 0.01; *** p < 0.001, compared to nontreated cells. # p < 0.05; ## p < 0.01; ### p < 0.001. (D, E, F, G) Cells were treated with 5 μM palbociclib, 5 μM CCT, or both drugs in combination for 48 h. Protein expression levels of OCT4 and c‐MYC (D, E), or ELF4, TRIB3, and β‐catenin (F, G), were analyzed by western blot. Quantitative data of band intensities in (E) and (G) resulted from three independent experimental repeats are quantified by ImageJ and are presented as mean ± SEM. represent results. * p < 0.05; ** p < 0.01; *** p < 0.001, compared to non‐treated cells. # p < 0.05; ## p < 0.01; ### p < 0.001. n.s., not significant.

Article Snippet: The human ELF4 cDNA (RefSeq NM_001421 ) tagged with Myc‐DDK was obtained from OriGene Technologies (Cat. No. RC200479, Rockville, MD, USA).

Techniques: Inhibition, MTT Assay, Inverted Microscopy, Expressing, Western Blot

Journal: Journal of Cellular Physiology

Article Title: ELF4/TRIB3/CDK6 Axis Promotes Cancer Stem Cell Activity in Endometrial Cancer

doi: 10.1002/jcp.70113

Figure Lengend Snippet: ELF4 and CDK6 expressions show a positive correlation in EC specimens. (A) Representative IHC images of ELF4, TRIB3, and CDK6 staining intensities in slides of an EC tissue microarray. (B) Pearson's correlation analysis of ELF4 and TRIB3 expressions in EC specimens. (C) Pearson's correlation analysis of ELF4 and CDK6 expression in EC specimens. (D) Pearson's correlation analysis of TRIB3 and CDK6 expression in EC specimens. (E) Overall survival (OS) curves for UCEC patients in the TCGA database, stratified by co‐expression of ELF4, TRIB3, and CDK6 mRNA (upper tertile cutoff), generated using the Kaplan‐Meier plotter tool. Significance assessed by log‐rank test.

Article Snippet: The human ELF4 cDNA (RefSeq NM_001421 ) tagged with Myc‐DDK was obtained from OriGene Technologies (Cat. No. RC200479, Rockville, MD, USA).

Techniques: Staining, Microarray, Expressing, Generated

Journal: RNA Biology

Article Title: E2-regulated transcriptome complexity revealed by long-read direct RNA sequencing: from isoform discovery to truncated proteins

doi: 10.1080/15476286.2025.2563860

Figure Lengend Snippet: A. Analysis of single-cell RNA sequencing data of GSE75688 verified the expression of IPA isoforms of TLE1 and GREB1 in breast cancer cells representing subtypes, B. GEO2R expression data for TLE1 -IPA (228284_at probe set) and FL isoforms (203221_at probe set) in NCI-60 cancer cell line data ( GSE32474 , GPL570 ). Red bars indicate breast cancer cell lines, C. Probe sets specific to IPA (228284_at) and FL (203221_at) in control cells compared with ER knockdown (KD) MCF7 cells ( GSE27473 ) (*** p < 0.001, students t-test), D. IPA/FL ratios were determined in breast cancer cell lines by RT-qPCR ( n = 3 technical replicates). IPA and FL expression levels were normalized to RPLP0. IPA/FL ratio was normalized to normal breast cDNA sample (from OriGene TissueScan cDNA tissue array) (*** p < 0.001, one-way ANOVA), E. TCGA breast cancer dataset showing a positive correlation between the levels of the FL and IPA isoforms of TLE1 , F. Kaplan-Meier relapse-free survival curves comparing high- and low-ratio of ENST00000376463.2/ENST00000376499.7 (IPA/FL) TLE1 in TCGA LumA breast cancers (BRCA). High-ratio patients (red) showed better relapse-free survival compared to low-ratio patients (black) (HR = 0.35, log-rank p = 0.042), G. Kaplan–Meier relapse-free survival analysis of LumA breast cancers, classified according to the St. Gallen criteria, was performed using microarray data from the KM-plotter database. Patients were stratified based on the expression ratio of the 228284_at probe set (IPA isoform) to the 203221_at probe set (FL isoform). High-ratio group (red) exhibited better relapse-free survival compared to the low-ratio group (black) (HR = 0.65, log-rank p = 0.0024). Patient numbers and at-risk counts are displayed on the plot.

Article Snippet: IPA/FL ratio was normalized to normal breast cDNA sample (from OriGene TissueScan cDNA tissue array) (*** p < 0.001, one-way ANOVA), E. TCGA breast cancer dataset showing a positive correlation between the levels of the FL and IPA isoforms of TLE1 , F. Kaplan-Meier relapse-free survival curves comparing high- and low-ratio of ENST00000376463.2/ENST00000376499.7 (IPA/FL) TLE1 in TCGA LumA breast cancers (BRCA).

Techniques: RNA Sequencing, Expressing, Control, Knockdown, Quantitative RT-PCR, Microarray