Journal: Journal of Functional Biomaterials

Article Title: Comparative Analysis of Osteointegration in Hydroxyapatite and Hydroxyapatite-Titanium Implants: An In Vivo Rabbit Model Study

doi: 10.3390/jfb15070181

Figure Lengend Snippet: Adhesion of MSCs 5 days post-cultivation on test materials HAp (left) and HApTi (right). The entire specimen was reconstructed by scanning the samples using the TissueFAXSiPlus system ( A ). A representative individual field obtained with the microscope’s 20× objective is shown ( B ). Detailed images of the cells can be observed at 40× magnification using the Zeiss Axio Imager microscope. Actin was highlighted using Alexa Fluor 488 Phalloidin (green), vinculin with Alexa Fluor 594 (red), and nuclei were stained with Hoechst (blue) ( C ).

Article Snippet: The TissueFAXSiPlus technology platform from TissueGnostics (Vienna, Austria) enables the scanning and reconstruction of specimens mounted on microscope slides utilizing the TissueFAXS Slides module and PCO PixelFly camera under standardized conditions.

Techniques: Microscopy, Staining

Journal: Nature Communications

Article Title: Enhanced and sustained biodistribution of HIV-1 neutralizing antibody VRC01LS in human genital and rectal mucosa

doi: 10.1038/s41467-024-54580-9

Figure Lengend Snippet: PAXgene-fixed, paraffin-embedded rectal tissue biopsies were sectioned (4 μm) and stained with 5C9 (VRC01 and VRC01LS detection) or mouse IgG2a (isotype control), followed by anti-mouse IgG-DAB (brown staining), and counterstained with hematoxylin (blue). All images were acquired on the TissueFAXS microscope in a bright field using a 10 × objective and identical exposure times. a Whole tissue section images of two representative individuals from VRC01-infused ( n = 8) and VRC01LS-infused recipients ( n = 4) are shown at multiple time points. Whole tissue section images indicate 5C9 staining of VRC01 or VRC01LS with 500 μm measurements to indicate the size of the tissue section analyzed; inset images are consecutive sections to the 5C9 stains, stained with mouse IgG isotype control. b – e Sections from 4 different participants at high magnification, with adjacent isotype control stained images. b , c Sections of rectal biopsies from 2 different VRC01-infused participants at 5, 6 weeks post infusion. d , e Sections of rectal biopsies from 2 different VRC01LS-infused participants at 5, 6 weeks post infusion. Abbreviations: A: adherent mucus layer, GE: glandular epithelium, LP: lamina propria, MM: muscularis mucosa, *: selected epithelium depicting mAb staining. A 100 μm ruler indicates size. f Manual scoring of 5C9 staining at different time points post-infusion. The number of participant samples analyzed is indicated above each bar. Scores were subtracted for any mouse IgG2a background to remove any artifacts. No data was excluded.

Article Snippet: Stained sections were imaged using a semi-automated digital pathology TissueFAXS system (Tissuegnostics GmbH) containing a Zeiss Imager Z2 upright microscope (Carl Zeiss Microscopy) with an 8-slide motorized stage (Marzhauser Wetzlar GmbH).

Techniques: Staining, Control, Microscopy