Journal: iScience

Article Title: Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood

doi: 10.1016/j.isci.2022.104694

Figure Lengend Snippet: Surfactant levels and urinary protein in SSNS/MCD (A) Plasma SP-A in control and ten subjects with SSNS/MCD in consecutive relapse/remission cycles (from 2 to 4 cycles). (B) Plasma SP-D in control and ten subjects with SSNS/MCD in consecutive relapse/remission cycles (from 2 to 4 cycles). (C) Correlation analysis between plasma SP-A and urinary SIRPα levels in samples from two controls (black symbols) and ten subjects with SSNS/MCD in relapse (blue symbols) and remission (red symbols). (D) Correlation analysis between plasma SP-A and urinary protein in samples from two controls (black symbols) and ten subjects with SSNS/MCD in relapse (blue symbols) and remission (red symbols). (E) Representative western blot for SP-A in the urine of a control subject (C1) and three patients with MCD in relapse (Rl1 to Rl3) and remission (Rm1 to Rm3). Statistical Analysis: One-Way ANOVA for A and B. For (C and D) Pearson r correlation analysis.

Article Snippet: Mouse SP-A (SFTPA) ELISA detection kit , Novus Biologicals , NBP2-77693.

Techniques: Clinical Proteomics, Control, Western Blot

Journal: iScience

Article Title: Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood

doi: 10.1016/j.isci.2022.104694

Figure Lengend Snippet: Intranasal instillation of LPS and PolyI:C and proteinuria in mice (A) Schematic depicting the protocol to induce proteinuria. Saline (red) or a combination of LPS (80 μg) and Poly I:C (240 μg) (blue) is administered intranasally once daily for three consecutive days. Urine is collected every 6 h and 3 h after the fourth injection, plasma, lung, and kidney tissue are collected for analysis. (B) Plasma SP-A levels in unmanipulated (black) mice or receiving either saline (red) or LPS/Poly I:C combination (n = 5 mice per group). (C) Representative confocal images for SP-A (green) and synaptopodin (red) colocalization in kidney glomeruli of mice receiving saline or LPS/Poly I:C. Merged colocalization is shown in yellow. Size bar: 200 μm. Images are representative from n = 5 mice per group (D) Confocal analysis of SIRPα (green) and SP-A (red) colocalization in kidney glomeruli of mice receiving saline or LPS/Poly I:C. Merged colocalization is shown in yellow. Size bar: 200 μm. Images are representative from five mice. (E) Co-immunoprecipitation of SIRPα and detection of SP-A in kidney cortex extracts of unmanipulated, saline-exposed, and LPS/Poly I:C-exposed mice. Total input (500 ug) and amount retained in preclearance is shown in lanes one and two; n = 3 mice per group. (F) Six hour albumin excretion over the 72-h period in unmanipulated (black) mice receiving either saline or LPS/Poly I:C. (G) 72-h accumulated albumin excretion in the urine of the same mice in F). n = 5 mice per group. (H) Representative electron microscopy podocyte images from saline- or LPS/PolyI:C-treated mice for 24 h. Blue arrows represent areas of foot effacement. Statistical Analysis: One-Way ANOVA, Tukey post hoc analysis for (B) and (G), data are represented as mean ± SEM.

Article Snippet: Mouse SP-A (SFTPA) ELISA detection kit , Novus Biologicals , NBP2-77693.

Techniques: Saline, Injection, Clinical Proteomics, Immunoprecipitation, Electron Microscopy

Journal: iScience

Article Title: Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood

doi: 10.1016/j.isci.2022.104694

Figure Lengend Snippet:

Article Snippet: Mouse SP-A (SFTPA) ELISA detection kit , Novus Biologicals , NBP2-77693.

Techniques: Recombinant, Protease Inhibitor, Enzyme-linked Immunosorbent Assay