Journal: bioRxiv

Article Title: Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas

doi: 10.64898/2026.03.27.714741

Figure Lengend Snippet: (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.

Article Snippet: Following heat-mediated antigen retrieval, consecutive sections were stained for TIM3 (MAB23652, R&D Systems) and CD30 (M0751, Agilent Dako) both at a 1:50 dilution.

Techniques: Staining, Expressing

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Regulatory B cells require antigen recognition for effective allograft tolerance induction

doi: 10.1111/ajt.15739

Figure Lengend Snippet: C57BL/6 transgenic mice expressing the hen egg lysozyme–specific, MD4 BCR fail to develop tolerance to BALB/c islets when treated with anti-CD45RB and anti-TIM1, compared to WT C57BL/6, which reliably develop tolerance post antibody treatment. *P < .05. BCR, B cell receptor; WT, wild type

Article Snippet: Islet recipient mice also received 500 μg anti-mouse TIM1 (clone RMT1–10, BioXCell, West Lebanon, NH) ip on day 0, and 300 μg on days 1 and 5 following transplantation.

Techniques: Transgenic Assay, Expressing

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Regulatory B cells require antigen recognition for effective allograft tolerance induction

doi: 10.1111/ajt.15739

Figure Lengend Snippet: Phenotype of B cells from naive and long-term graft survivor (LTS) OBI mice. A, Surface staining of B cells from naive and LTS OBI mice show similar levels of TIM1 and CD5 and higher levels of CD40. B, Tolerant OBI B cells show an increased proportion of LAP+ (a marker for TGF-β production) B cells upon stimulation with LPS, PMA, and ionomycin. C, Tolerant OBI B cells, however, do not show upregulation of IL-10 under the same stimulation protocol. Data are representative of 3 experiments. ns, nonsignificant. *P < .05. LPS, lipopolysaccharide; MFI, median flourescence intensity; OBI, ovalbumin (OVA)-specific BCR mice; PMA, phorbol myristate acetate; TGF, transforming growth factor

Article Snippet: Islet recipient mice also received 500 μg anti-mouse TIM1 (clone RMT1–10, BioXCell, West Lebanon, NH) ip on day 0, and 300 μg on days 1 and 5 following transplantation.

Techniques: Staining, Marker