tigre Search Results


addgene plasmid  (Addgene inc)
92
Addgene inc addgene plasmid
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
addgene plasmid - by Bioz Stars, 2026-04
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homology arms  (Addgene inc)
93
Addgene inc homology arms
Homology Arms, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
homology arms - by Bioz Stars, 2026-04
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fucci fragment  (Addgene inc)
91
Addgene inc fucci fragment
Fucci Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
fucci fragment - by Bioz Stars, 2026-04
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tigre locus  (Addgene inc)
93
Addgene inc tigre locus
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Tigre Locus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tigre locus/product/Addgene inc
Average 93 stars, based on 1 article reviews
tigre locus - by Bioz Stars, 2026-04
93/100 stars
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pen395  (Addgene inc)
92
Addgene inc pen395
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Pen395, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pen395/product/Addgene inc
Average 92 stars, based on 1 article reviews
pen395 - by Bioz Stars, 2026-04
92/100 stars
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puromycin resistance cassette  (Addgene inc)
91
Addgene inc puromycin resistance cassette
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Puromycin Resistance Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/puromycin resistance cassette/product/Addgene inc
Average 91 stars, based on 1 article reviews
puromycin resistance cassette - by Bioz Stars, 2026-04
91/100 stars
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tigre-wt-for: 50-gtc agg cac aag agg tct aca ttc-30  (Microsynth ag)
90
Microsynth ag tigre-wt-for: 50-gtc agg cac aag agg tct aca ttc-30
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Tigre Wt For: 50 Gtc Agg Cac Aag Agg Tct Aca Ttc 30, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tigre-wt-for: 50-gtc agg cac aag agg tct aca ttc-30/product/Microsynth ag
Average 90 stars, based on 1 article reviews
tigre-wt-for: 50-gtc agg cac aag agg tct aca ttc-30 - by Bioz Stars, 2026-04
90/100 stars
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tigre dragon-p21  (Jackson Laboratory)
90
Jackson Laboratory tigre dragon-p21
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Tigre Dragon P21, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tigre dragon-p21/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
tigre dragon-p21 - by Bioz Stars, 2026-04
90/100 stars
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tigre system  (21st Century Therapeutics)
90
21st Century Therapeutics tigre system
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Tigre System, supplied by 21st Century Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tigre system/product/21st Century Therapeutics
Average 90 stars, based on 1 article reviews
tigre system - by Bioz Stars, 2026-04
90/100 stars
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reserva ecológica el tigre  (ProMab Inc)
90
ProMab Inc reserva ecológica el tigre
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Reserva Ecológica El Tigre, supplied by ProMab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reserva ecológica el tigre/product/ProMab Inc
Average 90 stars, based on 1 article reviews
reserva ecológica el tigre - by Bioz Stars, 2026-04
90/100 stars
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tigre-variancbct  (Varian Medical)
90
Varian Medical tigre-variancbct
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Tigre Variancbct, supplied by Varian Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tigre-variancbct/product/Varian Medical
Average 90 stars, based on 1 article reviews
tigre-variancbct - by Bioz Stars, 2026-04
90/100 stars
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tigre  (Verlag GmbH)
90
Verlag GmbH tigre
A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in <t>at</t> <t>Rosa26</t> . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at <t>Tigre</t> . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.
Tigre, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tigre/product/Verlag GmbH
Average 90 stars, based on 1 article reviews
tigre - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in at Rosa26 . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at Tigre . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.

Journal: bioRxiv

Article Title: Transcriptional perturbation of LINE-1 elements reveals their cis -regulatory potential

doi: 10.1101/2024.02.20.581275

Figure Lengend Snippet: A) Structure of the DOX-inducible LKF/AKF transgenes transfected in PGK-G10 cells for knock-in at Rosa26 . B) Following puromycin selection, colony picking and genotyping, 9 clones were analysed by qPCR for expression of the L1MdTf/L1MdA families using primers specific for the 5’UTR region, upon 48h of DOX treatment. C) Structure of the DOX-inducible dCas9-VPR transgene transfected in PGK-G10 cells for knock-in at Tigre . D) Following puromycin and blasticidin selections, colony picking and genotyping, 7 clones, including the control VPR-B7, were analysed for expression of L1MdTf/L1MdA upon DOX treatment (48h) by qPCR. Scissors indicate the approximate positions of gRNAs. TRE3G: promoter Tet-responsive element 3rd generation. rtTA 3G: DOX-inducible transactivator 3rd generation. PuroR: puromycin resistance gene. BlastiR: blasticidin resistance gene. hU6: human U6 promoter. mU6: mouse U6 promoter. ZF: zinc fingers.

Article Snippet: Donor plasmids used for knock-ins at the Rosa26 (pEN111) and Tigre locus (pEN366, Addgene #156432), as well as the Rosa26 - and Tigre -specific sgRNA-encoding plasmids (pX335-EN479, pX335-EN481 and pX330-EN1201 (Addgene #92144)) were all provided by E. Nora (UCSF).

Techniques: Transfection, Knock-In, Selection, Clone Assay, Expressing, Control, Zinc-Fingers