Journal: bioRxiv

Article Title: Influence of asymmetric microchannels in the structure and function of engineered neuronal circuits

doi: 10.1101/2024.07.09.602729

Figure Lengend Snippet: (A) Scanning electron microscopy (SEM) images of the fabricated designs’ main variants (“Rams; “Arrows”; “Tesla”). Low magnification images (top) encompass the entire microfluidic device design, while high magnification images (bottom) detail the respective motif design. (B) Widefield microscopy mosaic image (20× objective) of different neuronal populations (“Source” and “Target”) at 14 days in vitro, selectively expressing mRuby3 (red) and EGFP (cyan), cultured on a microfluidic device with the “Tesla” motif. (C) Schematics of the rationale for each motif design. Green, orange and red arrows indicate axonal outgrowth-permissive, re-routing and blocking capabilities, respectively.

Article Snippet: All recordings were obtained using a commercial MEA2100-256 system (Multichannel Systems MCS, Germany) mounted on an incubated (37 °C) inverted widefield microscope (Axiovert 200M, Zeiss or Eclipse Ti2-E, Nikon) stage supplied with humidified 5% CO 2 .

Techniques: Electron Microscopy, Microscopy, In Vitro, Expressing, Cell Culture, Blocking Assay

Journal: bioRxiv

Article Title: Influence of asymmetric microchannels in the structure and function of engineered neuronal circuits

doi: 10.1101/2024.07.09.602729

Figure Lengend Snippet: (A) Widefield microscopy mosaic images (20× objective) of representative neuronal monocultures (seeded on the “Target” compartment) at 14 days in vitro (DIV) for each design. All cultures are expressing EGFP (grayscale). Asterisks mark microchannels that failed to completely block axonal outgrowth before the straight segment of the microchannel (dashed line). B) Percentage of microchannels that completely block axonal outgrowth per design. Filled dots represent the cultures shown on A). C) Normalized average pixel intensity values along the microchannels. The average intensity profiles (and colorized shade for the SD) of 5-6 independent experiments (16 microchannels per experiment) are plotted together with a fitted exponential curve. Per design, a representative microchannel is colorized with the Turbo lookup table (LUT).

Article Snippet: All recordings were obtained using a commercial MEA2100-256 system (Multichannel Systems MCS, Germany) mounted on an incubated (37 °C) inverted widefield microscope (Axiovert 200M, Zeiss or Eclipse Ti2-E, Nikon) stage supplied with humidified 5% CO 2 .

Techniques: Microscopy, In Vitro, Expressing, Blocking Assay