Journal: International Journal of Molecular Sciences

Article Title: In Search for Low-Molecular-Weight Ligands of Human Serum Albumin That Affect Its Affinity for Monomeric Amyloid β Peptide

doi: 10.3390/ijms25094975

Figure Lengend Snippet: Information on the AD-associated LWMLs selected for experimental examination of their influence on HSA–Aβ interaction.

Article Snippet: Propranolol (cat. #P913470), levothyroxine (L-thyroxine) (J62606.03), prednisone (PHR1042), warfarin (A2250), and mefenamic acid (M4267) were at least 98% pure and purchased from Macklin, Thermo Fisher Scientific (Waltham, MA, USA), and Sigma–Aldrich (Burlington, MA, USA) (for the last three compounds), respectively.

Techniques: Binding Assay, Infection

Journal: International Journal of Molecular Sciences

Article Title: In Search for Low-Molecular-Weight Ligands of Human Serum Albumin That Affect Its Affinity for Monomeric Amyloid β Peptide

doi: 10.3390/ijms25094975

Figure Lengend Snippet: The kinetics of HSA association (0–300 s) with the Aβ40 monomer immobilized on SPR chip’s surface by amine coupling, as well as the dissociation of their complex (300–2700 s), monitored by SPR spectroscopy in the presence (panels ( A – E )) or absence ( F ) of the HSA ligands shown in : 2.5 mM prednisone (panel ( A )), 1 mM warfarin ( B ), 250 μM mefenamic acid ( C ), 15 μM levothyroxine ( D ), and 1 mM propranolol ( E ) (20 mM Tris-HCl, 150 mM NaCl, pH 7.4; 25 °C). The experimental curves are described within the heterogeneous ligand model (Equation (2)) (see for the fitting parameters).

Article Snippet: Propranolol (cat. #P913470), levothyroxine (L-thyroxine) (J62606.03), prednisone (PHR1042), warfarin (A2250), and mefenamic acid (M4267) were at least 98% pure and purchased from Macklin, Thermo Fisher Scientific (Waltham, MA, USA), and Sigma–Aldrich (Burlington, MA, USA) (for the last three compounds), respectively.

Techniques: Spectroscopy

Journal: International Journal of Molecular Sciences

Article Title: In Search for Low-Molecular-Weight Ligands of Human Serum Albumin That Affect Its Affinity for Monomeric Amyloid β Peptide

doi: 10.3390/ijms25094975

Figure Lengend Snippet: Parameters of HSA-Aβ40 interaction in the presence/absence of the HSA ligands shown in Table 1 determined by SPR technique using the heterogeneous ligand model (Equation (2)).

Article Snippet: Propranolol (cat. #P913470), levothyroxine (L-thyroxine) (J62606.03), prednisone (PHR1042), warfarin (A2250), and mefenamic acid (M4267) were at least 98% pure and purchased from Macklin, Thermo Fisher Scientific (Waltham, MA, USA), and Sigma–Aldrich (Burlington, MA, USA) (for the last three compounds), respectively.

Techniques: