Journal: Heliyon

Article Title: Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

doi: 10.1016/j.heliyon.2023.e21616

Figure Lengend Snippet: Associated co-expressed differential proteins in placenta and plasma among pregnant women with ICP and healthy pregnant women.

Article Snippet: The primary antibodies used in this study were anti -NECTIN1 (1:100 dilution, Cat. 24713-1-AP, ProteinTech), anti -SPINT1 (1:100 dilution, Cat. ab189511; Abcam), anti -SERPINA7 (1:300 dilution; Cat. 66454-1-Ig; ProteinTech), anti-CD320 (1:300 dilution; Cat. 10343-1-AP, ProteinTech), anti -ITIH3 (1:100 dilution, Cat. DF14157, Affinity), anti -ENPP2 (1:50 dilution, Cat. 14243-1-AP, ProteinTech), and anti -SERPIND1 (1:25 dilution, Cat. ab232859; Abcam, Cambridge, UK).

Techniques: Clinical Proteomics, Protease Inhibitor

Journal: Heliyon

Article Title: Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

doi: 10.1016/j.heliyon.2023.e21616

Figure Lengend Snippet: Correlation analysis of co-differentially expressed protein levels between placental and plasma samples. (A) Correlation analysis of RPS7 between placental and plasma samples. (B) Correlation analysis of NECTIN3 between placental and plasma samples. (C) Correlation analysis of NECTIN1 between placental and plasma samples. (D) Correlation analysis of SPINT1 between placental and plasma samples. (E) Correlation analysis of SERPINA7 between placental and plasma samples. (F) Correlation analysis of CD320 between placental and plasma samples. (G) Correlation analysis of ITIH3 between placental and plasma samples. (H) Correlation analysis of ENPP2 between placental and plasma samples. (I) Correlation analysis of SERPIND1 between placental and plasma samples.

Article Snippet: The primary antibodies used in this study were anti -NECTIN1 (1:100 dilution, Cat. 24713-1-AP, ProteinTech), anti -SPINT1 (1:100 dilution, Cat. ab189511; Abcam), anti -SERPINA7 (1:300 dilution; Cat. 66454-1-Ig; ProteinTech), anti-CD320 (1:300 dilution; Cat. 10343-1-AP, ProteinTech), anti -ITIH3 (1:100 dilution, Cat. DF14157, Affinity), anti -ENPP2 (1:50 dilution, Cat. 14243-1-AP, ProteinTech), and anti -SERPIND1 (1:25 dilution, Cat. ab232859; Abcam, Cambridge, UK).

Techniques: Clinical Proteomics

Journal: Heliyon

Article Title: Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

doi: 10.1016/j.heliyon.2023.e21616

Figure Lengend Snippet: Correlation analysis between ELISA and sequencing results and the validation of plasma differential proteins using ELISA. (A–E) The correlation analysis of SPINT1, SERPINA7, NECTIN1, SERPIND1, and ITIH3 between ELISA and sequencing results. (F) NECTIN1 levels in plasma samples obtained from ICP and control groups (P < 0.0001). (G) SPINT1 levels in plasma samples obtained from ICP and control groups (P < 0.0001). (H) ITIH3 levels in plasma samples obtained from ICP and control groups (P = 0.0001). (I) ENPP2 levels in plasma samples obtained from ICP and control groups (P < 0.0001). (J) SERPIND1 levels in plasma samples obtained from ICP and control groups (P < 0.0001); (****P < 0.0001, ***P < 0.001). ELISA, enzyme-linked immunosorbent assay; ICP, intrahepatic cholestasis of pregnancy.

Article Snippet: The primary antibodies used in this study were anti -NECTIN1 (1:100 dilution, Cat. 24713-1-AP, ProteinTech), anti -SPINT1 (1:100 dilution, Cat. ab189511; Abcam), anti -SERPINA7 (1:300 dilution; Cat. 66454-1-Ig; ProteinTech), anti-CD320 (1:300 dilution; Cat. 10343-1-AP, ProteinTech), anti -ITIH3 (1:100 dilution, Cat. DF14157, Affinity), anti -ENPP2 (1:50 dilution, Cat. 14243-1-AP, ProteinTech), and anti -SERPIND1 (1:25 dilution, Cat. ab232859; Abcam, Cambridge, UK).

Techniques: Enzyme-linked Immunosorbent Assay, Sequencing, Biomarker Discovery, Clinical Proteomics, Control

Journal: Heliyon

Article Title: Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

doi: 10.1016/j.heliyon.2023.e21616

Figure Lengend Snippet: Diagnostic value analysis of plasma proteins in ICP.

Article Snippet: The primary antibodies used in this study were anti -NECTIN1 (1:100 dilution, Cat. 24713-1-AP, ProteinTech), anti -SPINT1 (1:100 dilution, Cat. ab189511; Abcam), anti -SERPINA7 (1:300 dilution; Cat. 66454-1-Ig; ProteinTech), anti-CD320 (1:300 dilution; Cat. 10343-1-AP, ProteinTech), anti -ITIH3 (1:100 dilution, Cat. DF14157, Affinity), anti -ENPP2 (1:50 dilution, Cat. 14243-1-AP, ProteinTech), and anti -SERPIND1 (1:25 dilution, Cat. ab232859; Abcam, Cambridge, UK).

Techniques: Diagnostic Assay, Clinical Proteomics

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: Characterization of non-radiolabeled Thyroxine (T 4 ) uptake in cryopreserved rat hepatocyte suspensions: Pharmacokinetic implications for PFOA and PFOS chemical exposure

doi: 10.1016/j.tiv.2019.03.022

Figure Lengend Snippet: (A) Percent difference from 37°C control of T4 (1.0 μM) uptake in cryopreserved rat hepatocyte suspensions treated with varying concentrations of the serum protein transthyretin. Data represent the linear rate of T4 hepatic uptake (pmoles/106 cells/sec) determined from individual timelines conducted in triplicate within a single experiment. Error bars represent ± SD, asterisks denote statistically significant differences versus the temperature control regression slopes (*p<0.05). (B) Percent difference from 37°C control of free T4 (1.0 μM) levels determined by 10kDa filtration of running buffer solutions with varying transthyretin concentration levels (no hepatocytes). Error bars represent ± SD, asterisks denote statistically significant differences versus the temperature control (*p<0.05).

Article Snippet: The serum protein transthyretin (TTR) derived from human plasma was purchased from Athens Research & Technologies (Athens, GA).

Techniques: Control, Filtration, Concentration Assay

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: Characterization of non-radiolabeled Thyroxine (T 4 ) uptake in cryopreserved rat hepatocyte suspensions: Pharmacokinetic implications for PFOA and PFOS chemical exposure

doi: 10.1016/j.tiv.2019.03.022

Figure Lengend Snippet: (A) Percent difference from 37°C control of T4 (1.0 μM) uptake in cryopreserved rat hepatocyte suspension treated with varying PFOA concentrations containing the serum protein transthyretin (62.5 μg/mL). Data represent the linear rate of T4 hepatic uptake (pmoles/106 cells/sec) determined from individual timelines conducted in triplicate within a single experiment. Error bars represent ± SD, asterisks denote statistically significant differences versus temperature control regression slopes (*p<0.05, **p<0.01). (B) Percent difference from 37°C control of free T4 (1.0 μM) levels in the presence of (62.5 μg/mL) transthyretin determined by 10kDa filtration of running buffer solutions (no hepatocytes). Error bars represent ± SD, asterisks denote statistically significant differences versus the temperature control (*p<0.05).

Article Snippet: The serum protein transthyretin (TTR) derived from human plasma was purchased from Athens Research & Technologies (Athens, GA).

Techniques: Control, Suspension, Filtration

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: Characterization of non-radiolabeled Thyroxine (T 4 ) uptake in cryopreserved rat hepatocyte suspensions: Pharmacokinetic implications for PFOA and PFOS chemical exposure

doi: 10.1016/j.tiv.2019.03.022

Figure Lengend Snippet: (A) Percent difference from 37°C control of T4 (1.0 μM) uptake in cryopreserved rat hepatocyte suspensions treated with CsA, PFOA, PFOS and Octanoic Acid containing the serum protein transthyretin (62.5 μg/mL). Data represent the linear rate of T4 hepatic uptake (pmoles/106 cells/sec) determined from individual timelines conducted in triplicate within a single experiment. Error bars represent ± SD, asterisks denote statistically significant differences versus temperature control regression slopes (*p<0.05, **p<0.01). (B) Percent difference from 37°C control of free T4 (1.0 μM) levels in the presence of (62.5 μg/mL) transthyretin determined by 10kDa filtration of running buffer solutions (no hepatocytes). Error bars represent ± SD, asterisks denote statistically significant differences versus the temperature control (*p<0.05).

Article Snippet: The serum protein transthyretin (TTR) derived from human plasma was purchased from Athens Research & Technologies (Athens, GA).

Techniques: Control, Filtration