thy1 1 plasmid Search Results


rack1  (Addgene inc)
93
Addgene inc rack1
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Rack1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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addgene plasmids 131702  (Addgene inc)
92
Addgene inc addgene plasmids 131702
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Addgene Plasmids 131702, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna encoding flag smad7  (Addgene inc)
90
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Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Cdna Encoding Flag Smad7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a rao55  (Addgene inc)
92
Addgene inc a rao55
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
A Rao55, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plentiguide thy1 1  (Addgene inc)
93
Addgene inc plentiguide thy1 1
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Plentiguide Thy1 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thy1 1 plasmid  (Addgene inc)
93
Addgene inc thy1 1 plasmid
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Thy1 1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thy1 1 plasmid/product/Addgene inc
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thy1 1 plasmid - by Bioz Stars, 2026-04
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fresh virus  (Addgene inc)
93
Addgene inc fresh virus
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Fresh Virus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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addgene 119 plasmid  (Addgene inc)
86
Addgene inc addgene 119 plasmid
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Addgene 119 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids h2b snapf mturquoise2  (Addgene inc)
92
Addgene inc plasmids h2b snapf mturquoise2
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Plasmids H2b Snapf Mturquoise2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mscv flipi p2g thy1 1 dbl  (Addgene inc)
86
Addgene inc mscv flipi p2g thy1 1 dbl
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Mscv Flipi P2g Thy1 1 Dbl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thy1 1  (Addgene inc)
93
Addgene inc thy1 1
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Thy1 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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expression plasmids pmcsv-ires-thy1.1 dest  (Marburg GmbH)
90
Marburg GmbH expression plasmids pmcsv-ires-thy1.1 dest
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Expression Plasmids Pmcsv Ires Thy1.1 Dest, supplied by Marburg GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the RACK1–PP2A complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three

Journal: Nature

Article Title: AIM2 in regulatory T cells restrains autoimmune diseases.

doi: 10.1038/s41586-021-03231-w

Figure Lengend Snippet: Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the RACK1–PP2A complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three

Article Snippet: Ectopic expression of PP2A and RACK1 in Treg cells To generate the retrovirus expressing PP2A and RACK1, we first cloned PP2A (OriGene Technologies, MR204384L4) and RACK1 (OriGene Technologies, MR204575L3) into retroviral vectors MSCV-IRES-Thy1.1 (MIT, Addgene 17442) and MSCV-IRES-GFP (MIG, Addgene 20672) respectively, and generated MIT-PP2A and MIG-RACK1 retrovirus in 293T cells by transient transfection.

Techniques: In Vitro, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, RNA Sequencing, Western Blot