Journal: Neurochemical Research

Article Title: Morin Improves Cognitive Deficits in an in Vivo Model of Vascular Dementia by Modulating the N-methyl-D-aspartate Receptor Signaling Pathways

doi: 10.1007/s11064-026-04717-7

Figure Lengend Snippet: Morin modulated the expression of NMDA receptors in the hippocampus of VaD rats. a the expression levels of NR1 ; b the expression levels of NR2A ; c the expression levels of NR2B ; d the expression levels of NR1 protein; e the expression levels of NR2A protein; f the expression levels of NR2B protein; g protein levels of p-CREB; h protein levels of p-CAMK2A; i protein levels of p-CAMK2D. Protein levels of NR1, NR2A, and NR2B were quantified by ELISA. Data are presented as mean ± SD ( n = 8 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post-hoc test (data met assumptions of normality and homoscedasticity)/Kruskal-Wallis with Dunn’s test (data did not meet assumptions). * indicates a significant difference from the Sham group; # indicates a significant difference 2VO group; *# indicates a significant difference from both the Sham and 2VO groups, with a p-value of less than 0.05 considered statistically significant

Article Snippet: Moreover, phosphorylation levels of calcium/calmodulin-dependent protein kinase II isoforms CAMK2A and CAMK2D at Thr286 (p-CAMK2A, p-CAMK2D) were quantified using the Colorimetric Cell-Based ELISA Kit (CAMK2A/CAMK2D (Phospho-Thr286), Boster Bio, #EKC2366).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Conserved and divergent features of neuronal CaMKII holoenzyme structure, function, and high-order assembly

doi: 10.1016/j.celrep.2021.110168

Figure Lengend Snippet:

Article Snippet: pT286 , Phospho-Solutions , p1005–286; RRID: AB_2492051.

Techniques: Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Software, Imaging

Journal: Life Science Alliance

Article Title: Systems genetics analysis of human body fat distribution genes identifies adipocyte processes

doi: 10.26508/lsa.202402603

Figure Lengend Snippet: (A) Wnt transcriptional activity measured by luminescence of luciferase reporter (n = 3–6). (B, C) Representative images and (C) Quantification of active (non-phosphorylated) and total β-catenin by immunoblotting (n = 12). (D) Gene expression of AXIN2 measured by qRT-PCR (n = 3). (E) Ratio of active (phosphorylated): total GSK3β measured by ELISA (n = 3). (F) Gene expression of IL6 measured by qRT-PCR (n = 3). (G) Ratio of active (phosphorylated): total CAMK2A measured by ELISA (n = 2). (H) Ratio of active (phosphorylated): total JNK measured by ELISA (n = 3). Data Information: All plots show mean ± SEM. Differences between groups were determined using one-way ANOVA by gene (Gene of Interest versus GFP controls), and post hoc tests were performed using pooled t tests with Dunnett’s adjustment. Adjusted P -values shown with * (*** = adj. P < 0.001, ** = adj. P < 0.01, * = adj. P < 0.05).

Article Snippet: Active CAMK2A (Thr286 phosphorylated) and total CAMK2A were measured using an ELISA kit (Assay BioTechnology) using manufacturer’s protocols (Cat# FLUO-CBP1509 and CB5092).

Techniques: Activity Assay, Luciferase, Western Blot, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay