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Image Search Results
Journal: bioRxiv
Article Title: A Rosella-PLIN2 Knock-in Mouse Reveals Lipophagy and Immunometabolic Interplay in Atherosclerosis
doi: 10.1101/2025.04.15.647781
Figure Lengend Snippet: ( a ) Flow cytometry analysis of foamy (Lipid hi ) and non-foamy (Lipid lo ) cell populations, including Rosella + cells, endothelial (Endo), leukocytes (Leuko), macrophages (MΦ) and non-myeloid leukocytes (N-myeloid) in aortic arches from hypercholesterolemic Rosella-PLIN2 mice. ( b ) Percentage of Rosella + cells among Lipid hi and Lipid lo cells in aortic arches analysed in ( a ). (n=3 mice per sex pooled) ( c ) Percentage of indicated cell types among Lipid hi or Rosella + cells in aortic arches analysed in ( a ). ( d ) Percentage of Rosella + cells among Lipid hi cells in aortic arches analysed in ( a ). ( e ) Geometric mean fluorescence intensity (gMFI) of MDH in cells with high or low lipophagy flux among Rosella + cell types identified in ( a ). ( f-g ) gMFI of DsRed:pHluorin ratio ( f ) and MDH ( g ) in Rosella + plaque MΦ expressing (+) or not (-) indicated surface markers. ( h ) gMFI of DsRed:pHluorin ratio in thioglycolate-elicited peritoneal MΦ isolated from hypercholesterolemic Rosella-PLIN2 mice, expressing (+) or not (-) indicated surface markers. (n=3 female mice). Data are mean ± SEM, two-way ANOVA with Sidak post-hoc multiple comparison test.
Article Snippet: Peritoneal macrophages were harvested from 8-week-old male and female C57BL/6N mice 3 days after intraperitoneal injection of 1 ml 3%
Techniques: Flow Cytometry, Fluorescence, Expressing, Isolation, Comparison