Journal: Frontiers in Pediatrics

Article Title: Cytokine responses to SARS-COV2 infection in mother-infant dyads: a systematic review and meta-analysis

doi: 10.3389/fped.2023.1277697

Figure Lengend Snippet: Characteristics of included studies.

Article Snippet: Tartaglia et al. ( ) , Italy , Prospective case-control study , COVID recovered Pregnant women (pCOV), n = 17 , COVID recovered matched non- pregnant women (nCOV), n = 12 , Maternal serum samples, no timing mentioned , 11 cytokines, chemokines, and growth factors- GM-CSF, IFN-gamma, IL-1 beta/ IL-1F2, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 p70, IL-13, TNF-alpha, IL-17 , Luminex Human Th1/Th2 11-plex Fixed Panel, Human IL-17 immunoassay, biotechne.

Techniques: Enzyme-linked Immunosorbent Assay, Electrochemiluminescence, Luminex, Infection, Flow Cytometry

Journal: Advanced Science

Article Title: Electret‐Inspired Charge‐Injected Hydrogel for Scar‐Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation

doi: 10.1002/advs.202411889

Figure Lengend Snippet: Quantitative analysis of relative area percentage of a) T‐bet, b) CXCR3, and c) GATA3, respectively. Data were shown as mean ± SD (n=3,* p < 0.05 when compared with Blank group; # p < 0.05 when compared with QO group; & p < 0.05 when compared with QOS group). The relative mRNA expression levels of (d) CXCR3 and e) IFN‐γ in Jurkat E6‐1 cells, analyzed by real‐time qPCR. CXCR3 and IFN‐γ are specific markers of Th1 cells, indicating the expression level of Th1 cells.

Article Snippet: Th1 conditions: IL‐12 (20 ng mL− 1 ), IL‐2 (20 ng mL− 1 ), anti‐IL‐4 (5 ng mL− 1 ); Th2 conditions: IL‐4 (20 ng mL− 1 ), IL‐2 (20 ng mL− 1 ), anti‐IFN‐γ (5 ng mL− 1 ), all purchased from Proteintech, Wuhan, China.

Techniques: Expressing

Journal: Advanced Science

Article Title: Electret‐Inspired Charge‐Injected Hydrogel for Scar‐Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation

doi: 10.1002/advs.202411889

Figure Lengend Snippet: Mechanism diagram of QOSP hydrogel regulating Th1/Th2 balance.

Article Snippet: Th1 conditions: IL‐12 (20 ng mL− 1 ), IL‐2 (20 ng mL− 1 ), anti‐IL‐4 (5 ng mL− 1 ); Th2 conditions: IL‐4 (20 ng mL− 1 ), IL‐2 (20 ng mL− 1 ), anti‐IFN‐γ (5 ng mL− 1 ), all purchased from Proteintech, Wuhan, China.

Techniques:

Journal: Frontiers in Immunology

Article Title: Peripheral blood mononuclear cell low molecular mass protein 7 in acute ischemic stroke: vertical change from admission to discharge and correlation with disability, stroke recurrence, and death

doi: 10.3389/fimmu.2024.1296835

Figure Lengend Snippet: Characteristics at admission.

Article Snippet: Then those proportions were determined by flow cytometric (FCM) analysis (FlowX Human Th1, Th2, and Th17 Cell Multi-Color Flow Cytometry Kits, No. Cat. FMC009B, FMC011B, and FMC007B, Bio-Techne, China).

Techniques:

Journal: Frontiers in Immunology

Article Title: Peripheral blood mononuclear cell low molecular mass protein 7 in acute ischemic stroke: vertical change from admission to discharge and correlation with disability, stroke recurrence, and death

doi: 10.3389/fimmu.2024.1296835

Figure Lengend Snippet: Elevated PBMC LMP7 at admission was correlated with decreased Th2 cells and increased Th17 cells, CRP, and NIHSS score in AIS patients. The correlation of PBMC LMP7 at admission with Th1 cells (A) , Th2 cells (B) , Th17 cells (C) , CRP (D) , CRP ≥5 mg/L (E) , NIHSS score (F) , and stroke severity classified by NIHSS score (G) .

Article Snippet: Then those proportions were determined by flow cytometric (FCM) analysis (FlowX Human Th1, Th2, and Th17 Cell Multi-Color Flow Cytometry Kits, No. Cat. FMC009B, FMC011B, and FMC007B, Bio-Techne, China).

Techniques:

Journal: Journal of molecular biology

Article Title: The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes

doi: 10.1016/j.jmb.2013.09.012

Figure Lengend Snippet: B factors (Panel A) were calculated from the aqueous simulations for TH-(1-43) (black line) and THp-(1-43) (red line) and from the peptide/membrane simulations for TH-(1-43) (blue line) and THp-(1-43) (green line), and are represented as a function of residue number. Panel B: Representative structure of THp-(1-43) which shows the intrapeptide sidechain-sidechain hydrogen bonds with occupancies >25% (dashed lines) in the aqueous simulation. The peptide is coloured as a continuous gradient going from blue (N-terminal) to red (C-terminal). The last 100 ns of each simulation were used for both analyses.

Article Snippet: TH peptides in water and with the POPS membrane The peptides TH-(1-43) and THp-(1-43) were built as random coils with the protein builder in Accelrys Discovery Studio 36 and simulated for 100 ns in explicit octahedral-shaped water boxes.

Techniques:

Journal: Journal of molecular biology

Article Title: The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes

doi: 10.1016/j.jmb.2013.09.012

Figure Lengend Snippet: A) Representative sensorgrams for the interaction of TH-(1-43) with POPS liposomes on a L1 sensor chip at increasing concentrations of peptide. B) Peptide concentration dependency of TH-(1-43) (black) and THp-(1-43) (red) when bound to POPS liposomes. RUs were measured as a function of peptide concentration. S0.5 values were extracted from the hyperbolic, single-rectangular, two-parameter curve fitting and resulted in S0.5 of 53.3 ± 9.2 µM and 48.2 ± 7.4 µM for TH-(1-43) and THp-(1-43), respectively. C) Far-UV CD spectra of 40 µM TH-(1-43) (black lines) or THp-(1-43) (red lines) without (solid lines) or with (dotted lines) POPS liposomes (0.6 mM phospholipid). Samples were prepared in 10 mM Na-Hepes, 150 mM NaCl, pH 7.4, and spectra were recorded at 25 °C.

Article Snippet: TH peptides in water and with the POPS membrane The peptides TH-(1-43) and THp-(1-43) were built as random coils with the protein builder in Accelrys Discovery Studio 36 and simulated for 100 ns in explicit octahedral-shaped water boxes.

Techniques: Concentration Assay

Journal: Journal of molecular biology

Article Title: The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes

doi: 10.1016/j.jmb.2013.09.012

Figure Lengend Snippet: TH-(1-43)/POPS is presented in panels A and B, and THp-(1-43)/POPS is presented in panels C and D. Panels A and C show the peptide – coloured as a continuous gradient going from blue (N-terminal) to red (C-terminal) – together with most of the peptideinteracting leaflet of the POPS bilayer, the orange spheres representing the head group phosphorus atoms. Panels B and D provide close-ups of the most important hydrogen bond interactions (dashed lines) in each case, where the relevant TH residues are displayed as ball-and-stick models and the POPS lipids as sticks. Water and ions have been removed for clarity.

Article Snippet: TH peptides in water and with the POPS membrane The peptides TH-(1-43) and THp-(1-43) were built as random coils with the protein builder in Accelrys Discovery Studio 36 and simulated for 100 ns in explicit octahedral-shaped water boxes.

Techniques:

Journal: Journal of molecular biology

Article Title: The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes

doi: 10.1016/j.jmb.2013.09.012

Figure Lengend Snippet: The energies are computed using 1000 frames extracted from the last 100 ns of each peptide/membrane simulation (systems D and E in Table 1). ΔGdecomp = ΔG’ – ΔGnon-polar. The black bars represent the energies derived for each residue in TH-(1-43), and the grey bars are the THp-(1-43) equivalents. A) Sorted by amino acid sequence; B) Sorted in ascending order according to the TH-(1-43) decomposition energies.

Article Snippet: TH peptides in water and with the POPS membrane The peptides TH-(1-43) and THp-(1-43) were built as random coils with the protein builder in Accelrys Discovery Studio 36 and simulated for 100 ns in explicit octahedral-shaped water boxes.

Techniques: Derivative Assay, Sequencing