Journal: Biomolecules

Article Title: GDF-15 Inhibits ADP-Induced Human Platelet Aggregation through the GFRAL/RET Signaling Complex

doi: 10.3390/biom14010038

Figure Lengend Snippet: The expression of receptors associated with GDF-15 on platelets. ( A ) GFRAL and RET were detected in platelets. ( B ) TGF-βRI rather than TGF-βRII was expressed on platelets. ( C ) Among the three members of the EGFR family associated with GDF-15, EGFR, and HER3 were expressed on platelets. n = 6. Original images of ( A – C ) can be found in .

Article Snippet: According to a previously described method [ ], the GDF-15-related receptor microarray comprises five recombinant human proteins, including those rearranged during transfection (RET, 11997-H08H1, Sino Biological, Beijing, China), the epidermal growth factor receptor (EGFR, 10001-H08S, Sino Biological, Beijing, China), human epidermal growth factor receptor 3 (HER3, 10201-HCCH, Sino Biological, Beijing, China), TGF-βRI (10459-H02H, Sino Biological, Beijing, China), and GFRAL (9467-GR, R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing

Journal: Biomolecules

Article Title: GDF-15 Inhibits ADP-Induced Human Platelet Aggregation through the GFRAL/RET Signaling Complex

doi: 10.3390/biom14010038

Figure Lengend Snippet: The screening for proteins that interact with GDF-15 among the receptors expressed on human platelets. ( A ) The results of the GDF-15-related receptor microarray detecting direct interactions between receptors (GFRAL, RET, EGFR, HER3, and TGF-βRI) and GDF-15 at 0.1 μg/mL, 1 μg/mL, and 10 μg/mL Biotin was used for the negative control. ( B ) The immunoprecipitation assay revealed an interaction between GDF-15 and GFRAL in human platelets. Rabbit IgG was used for isotype control. VeriBlot for the IP detection reagent, which only recognizes native (non-reduced) antibodies, was used for immunoblotting. Red box: the Western Blot band for GFRAL. Original images of ( B ) can be found in .

Article Snippet: According to a previously described method [ ], the GDF-15-related receptor microarray comprises five recombinant human proteins, including those rearranged during transfection (RET, 11997-H08H1, Sino Biological, Beijing, China), the epidermal growth factor receptor (EGFR, 10001-H08S, Sino Biological, Beijing, China), human epidermal growth factor receptor 3 (HER3, 10201-HCCH, Sino Biological, Beijing, China), TGF-βRI (10459-H02H, Sino Biological, Beijing, China), and GFRAL (9467-GR, R&D Systems, Minneapolis, MN, USA).

Techniques: Microarray, Negative Control, Immunoprecipitation, Western Blot

Journal: Brain Communications

Article Title: The influence of the R47H triggering receptor expressed on myeloid cells 2 variant on microglial exosome profiles

doi: 10.1093/braincomms/fcab009

Figure Lengend Snippet: Microglial gene signature primer details

Article Snippet: TGFBR1 , Hs00610320_m1.

Techniques:

Journal: Journal of translational autoimmunity

Article Title: Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells.

doi: 10.1016/j.jtauto.2023.100202

Figure Lengend Snippet: Fig. 3. Immunofluorescent antibody staining in naïve and treated HUVECs. AK. The mixture of APS total IgG and β2GPI induces a pronounced increase of the protein levels of the proinflammatory cytokines IL-6, IL-8 as well the transcription factor NF-κB1 and cell adhesion molecules Tissue Factor, ICAM-1, VCAM-1, Eselectin, P- selectin and TGFR1. There was no significant difference for the TGFR1 molecule (3 J). Visual analysis revealed that all the inflammatory mediators and adhesion molecules presented statistically significant difference between the untreated and treated endothelial cells (3 K).

Article Snippet: Coverslips were incubated overnight at 4 ◦C with primary antibodies against IL-6 (5 μg/ ml, CSB-PA06757A0Rb, Cusabio), IL-8 (5 μg/ml, CSB-MA083271A0m, Cusabio), NF-κB1 (5 μg/ml, CSB-PA190132, Cusabio), TGF-β2 (5 μg/ ml, CSB- PA07319A0Rb, Cusabio), Tissue Factor (5 μg/ml, 4509, American Diagnostica), ICAM-1 (5 μg/ml, AF796, R&D Systems), VCAM-1 (4 μg/ml, sc-18854, Santa Cruz Biotechnology), E-selectin (4 μg/ml, sc-271267, Santa Cruz Biotechnology), P-selectin (4 μg/ml,sc137054, Santa Cruz Biotechnology) and TGFR1 (5 μg/ml,CSBPA023451LA01HU, Cusabio).

Techniques: Staining

Journal: Journal of translational autoimmunity

Article Title: Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells.

doi: 10.1016/j.jtauto.2023.100202

Figure Lengend Snippet: Fig. 5. Immunofluorescent antibody staining in placenta biopsies from APS patients and healthy women. A-K Placenta biopsies derived from APS patients as well as Healthy Donors show increased signal intensity for IL-6, IL-8, NF-κB1, ICAM1, VCAM-1, E-selectin, P-selectin, TGF-β2, and TGFR1 (5A-5D, 5F-5J). Slight difference in fluorescence intensity between HD and APS patient was observed for Tissue Factor (5E). Increased signal intensity was observed as well for the TNF-α molecule in the APS placenta biopsies (5 K).

Article Snippet: Coverslips were incubated overnight at 4 ◦C with primary antibodies against IL-6 (5 μg/ ml, CSB-PA06757A0Rb, Cusabio), IL-8 (5 μg/ml, CSB-MA083271A0m, Cusabio), NF-κB1 (5 μg/ml, CSB-PA190132, Cusabio), TGF-β2 (5 μg/ ml, CSB- PA07319A0Rb, Cusabio), Tissue Factor (5 μg/ml, 4509, American Diagnostica), ICAM-1 (5 μg/ml, AF796, R&D Systems), VCAM-1 (4 μg/ml, sc-18854, Santa Cruz Biotechnology), E-selectin (4 μg/ml, sc-271267, Santa Cruz Biotechnology), P-selectin (4 μg/ml,sc137054, Santa Cruz Biotechnology) and TGFR1 (5 μg/ml,CSBPA023451LA01HU, Cusabio).

Techniques: Staining, Derivative Assay, Fluorescence

Journal: Scientific Reports

Article Title: Triterpenoid modulation of IL-17 and Nrf-2 expression ameliorates neuroinflammation and promotes remyelination in autoimmune encephalomyelitis

doi: 10.1038/srep00201

Figure Lengend Snippet: Quantitative PCR probes

Article Snippet: Tgfbr1 , Mm00436971_m1 , ALK5; Alk-5; TbetaRI.

Techniques: Real-time Polymerase Chain Reaction

Journal: bioRxiv

Article Title: Microglia-derived TGF-β1 ligand maintains microglia homeostasis via autocrine mechanism and is critical for normal cognitive function in adult mouse brain

doi: 10.1101/2023.07.05.547814

Figure Lengend Snippet: Key Resources Table

Article Snippet: Tgfbr1 qrtPCR assay (Mm00436965_m1) , ThermoFisher , Cat#4351372.

Techniques: Recombinant, Staining, Isolation, cDNA Synthesis, Software, Microscopy, Flow Cytometry