Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Magnesium isoglycyrrhizinate ameliorates radiation-induced pulmonary fibrosis by inhibiting fibroblast differentiation via the p38MAPK/Akt/Nox4 pathway.

doi: 10.1016/j.biopha.2019.108955

Figure Lengend Snippet: Fig. 4. MgIG regulated the expression of TGF-β1 and Nox4, and the phosphorylation of p38MAPK and Akt in vivo. The protein level of TGF-β1 in lung tissues at 12 weeks post-irradiation was determined using (A) im- munohistochemical staining (×200, n = 6) and (B) western blotting (n = 3), and quanti- tative analyses were performed. (C) TGF-β1 content in serum at 12 weeks post-irradiation was measured using an ELISA kit (n = 6). The protein levels of Nox4, and the phosphoryla- tion of p38MAPK and Akt in lung tissues at 12 weeks post-irradiation were observed using (D) immunohistochemical staining (×200, n = 6) and (E) western blotting (n = 3), and quanti- tative analyses were performed. All data were expressed as the mean ± SEM. #P < 0.05 vs. the control group; *P < 0.05 vs. the IR group; &P < 0.05 vs. the IR + MgIG group; Bar =50 μm.

Article Snippet: The serum was used to measure the TGF-β1 concentration using an ELISA kit (EK0515, Boster Bioengineering Institute, Huhan, China), according to the manufacturer's instructions.

Techniques: Expressing, Phospho-proteomics, In Vivo, Irradiation, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Control

Journal: Cell death & disease

Article Title: FSTL1 interacts with VIM and promotes colorectal cancer metastasis via activating the focal adhesion signalling pathway.

doi: 10.1038/s41419-018-0695-6

Figure Lengend Snippet: Fig. 5 TGFβ1-Smad2/3 signalling pathway regulates the expression of FSTL1 through activating the transcriptional activity of Smad3 in human CRC. a Representative TGF-β1 and FSTL1 immunohistochemistry staining photographs of normal tissue (Normal) and tumour tissue samples (Tumour 1, Tumour 2), (×200, scale = 50 μm). The TGF-β1 and FSTL1 were spatially correlated. b TGF-β1 activated Smad2/3 signalling and enhanced FSTL1 expression in DLD1 and RKO cells by western blotting. SB431542 attenuated the expression of P-smad2, P-smad3 and FSTL1. c Schematic of the FSTL1 promoter luciferase construct is depicted with the locations of binding site and the sequences of mutation (left). Luciferase activities in HEK293T and SW480 cells were examined after transfecting with Smad3 (right), each P < 0.001. Error bars represent the mean ± S.D. of the ratio of firefly and renilla luciferase signals (n = 3). d Smad3 binding on the promoter region of FSTL1 was assessed by ChIP assay. Immunoprecipitation from SW480 cells using Smad3 antibody or rabbit immunoglobulin G (IgG). PCR from the IP samples using FSTL1-specific primers. e Western blotting analysis of Smad3 and FSTL1 in Lovo and SW480 cells, after using smad3-siRNA mediated RNA interference. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: IHC was performed on paraffin sections of CRC tissues according to standard LSAB protocol (Dako), using primary antibodies against FSTL1, TGF-β1 (Proteintech, USA) respectively.

Techniques: Expressing, Activity Assay, Immunohistochemistry, Staining, Western Blot, Luciferase, Construct, Binding Assay, Mutagenesis, Immunoprecipitation