Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: MicroRNA-150 relieves vascular remodeling and fibrosis in hypoxia-induced pulmonary hypertension.

doi: 10.1016/j.biopha.2018.11.058

Figure Lengend Snippet: Fig. 4. Effect of miR-150 on pulmonary fibrosis of pulmonary hypertension rats. (A) Pulmonary fibrosis was detected by Masson’s staining (200× magnification). Scale bars, 100 μm. (B) The area of pulmonary fibrosis was calculated and shown. The mRNA expressions of TGF-β1 (C) and collagen I (D) in lung tissues were evaluated by qPCR. (E) The protein levels of TGF-β1 and collagen I in lung tissues were measured by western blot assay. (F)&(G) Relative grey values of the protein bands were shown. (H) The expressions of TGF-β1 and collagen I in lung tissues were detected by immunohistochemical staining (400× magnification). Scale bars, 50 μm. Data were presented as mean ± SD. **P < 0.01, ***P < 0.001 versus the indicated group.

Article Snippet: The membranes were blocked in 5% nonfat milk for 1 h at room temperature, then incubated with primary antibodies against TGF-β1 (1:400, BOSTER, China), Collagen I (1:300, BOSTER, China), p-AKTser473 (1:500, KeyGen, China), AKT (1:500, KeyGen, China), p-mTORser2481 (1:500, Sangon Biotech, China), mTOR (1:1000, Cell signaling Technology, USA), and β-actin (1:500, Bioss, China) at 4°C overnight, followed by incubation with HRP-labeled Goat AntiRabbit or Goat Anti-Mouse IgG (1:5000, Beyotime, China) at 37°C for 45min.

Techniques: Staining, Western Blot, Immunohistochemical staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: MicroRNA-150 relieves vascular remodeling and fibrosis in hypoxia-induced pulmonary hypertension.

doi: 10.1016/j.biopha.2018.11.058

Figure Lengend Snippet: Fig. 6. Effect of miR-150 on the expressions of fibrosis-related molecules. The mRNA expressions of TGF-β1 (A) and collagen I (B) in PASMCs were measured by qPCR. (C) The protein levels of TGF-β1 and collagen I in PASMCs were detected by western blot assay. (D)&(E) Relative grey values of the protein bands were shown. Data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus the indicated group.

Article Snippet: The membranes were blocked in 5% nonfat milk for 1 h at room temperature, then incubated with primary antibodies against TGF-β1 (1:400, BOSTER, China), Collagen I (1:300, BOSTER, China), p-AKTser473 (1:500, KeyGen, China), AKT (1:500, KeyGen, China), p-mTORser2481 (1:500, Sangon Biotech, China), mTOR (1:1000, Cell signaling Technology, USA), and β-actin (1:500, Bioss, China) at 4°C overnight, followed by incubation with HRP-labeled Goat AntiRabbit or Goat Anti-Mouse IgG (1:5000, Beyotime, China) at 37°C for 45min.

Techniques: Western Blot

Journal: Journal of hazardous materials

Article Title: Thyroid cancer risk associated with perfluoroalkyl carboxylate exposure: Assessment using a human dermal fibroblast-derived extracellular matrix-based thyroid cancer organoid.

doi: 10.1016/j.jhazmat.2024.135771

Figure Lengend Snippet: Fig. 1. Establishment of thyroid cancer organoids and biomarker analysis (A) Schematic diagram for the fabrication of thyroid cancer organoids derived from the human thyroid cancer cell line SNU790. (B) Confocal microscopy z-stage imaging of established thyroid cancer organoid cultures for morphological and biomarker (TSHR, F-actin) analysis. Scale bar = 100 µm. (C) Immunofluorescence microscopy images of thyroid cancer organoids expressing thyroglobulin (Tg), captured using the Operetta™ CLS high-content imaging system. Scale bar = 100 µm. (D) E-cadherin expression: confocal microscopy z-stage imaging of human thyroid cancer organoids following 21-day culture. Scale bar = 100 µm. (E) Immunofluorescence microscopy images of thyroid cancer organoids exhibiting expression of vimentin captured with the Operetta™ CLS high-content imaging system. Scale bar = 100 µm. ECM, extracellular matrix.

Article Snippet: The antibodies against thyroid-stimulating hormone receptor (TSHR; #14450-1-AP) and thyroglobulin (Tg; #21714-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Biomarker Discovery, Derivative Assay, Confocal Microscopy, Imaging, Immunofluorescence, Microscopy, Expressing

Journal: Journal of hazardous materials

Article Title: Thyroid cancer risk associated with perfluoroalkyl carboxylate exposure: Assessment using a human dermal fibroblast-derived extracellular matrix-based thyroid cancer organoid.

doi: 10.1016/j.jhazmat.2024.135771

Figure Lengend Snippet: Fig. 3. Changes in the levels of thyroid cancer biomarkers following long-term PFAC exposure. (A) Thyroid-stimulating hormone receptor (TSHR) visuali zation: immunofluorescence photomicrographs showing TSHR expression in human thyroid cancer organoids treated with 10 µM of PFACs for a period of 21 days. Scale bar = 100 µm for size reference. (B) Western blot of TSHR and β-actin in thyroid cancer organoids treated with different PFAC types for 21 days. (C) Thyroglobulin (Tg) staining: immunofluorescence staining micrographs demonstrate the presence of Tg in human thyroid cancer organoids. Images were captured using an Operetta™ CLS High-Content Imaging System and an Olympus confocal microscope. Scale bar = 100 µm for gauging the size and scale of structures. (D) Western blot of Tg and β-actin expression in thyroid cancer organoids treated with the control and different PFAC types (10 µM) for 21 days. PFBA, perfluorobutanoic acid; PFPeA, perfluoropentanoic acid; PFOA, perfluorooctanoic acid; PFDA, perfluorodecanoic acid; CTL, control.

Article Snippet: The antibodies against thyroid-stimulating hormone receptor (TSHR; #14450-1-AP) and thyroglobulin (Tg; #21714-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Immunofluorescence, Expressing, Western Blot, Staining, Imaging, Microscopy, Control

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Dose response of LPS-induced lethality and TNF-α activity in serum in rabbits primed with TSST-1

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Activity Assay

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Time course of LPS-induced TNF-α in serum in Dutch belted rabbits primed with TSST-1. Groups of three rabbits were injected with 10.0 ng (i.v.) of TSST-1 (primed)/kg or an equivalent volume of PBS (unprimed). All rabbits were injected with LPS (10 μg/kg [i.v.]) 12 h later. Control rabbits injected with 5 μg of TSST-1 and PBS/kg 12 h later did not develop detectable levels of TNF-α. (∗, P ≤ 0.05).

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Injection, Control

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Direct comparison of LPS-induced TNF-α in serum in mice and rabbits primed with TSST-1. Groups of three BALB/c-AnNCr mice and three Dutch belted rabbits were injected with TSST-1 at doses of 200 μg/kg (i.p.) or 10.0 ng/kg (i.v.), respectively. After 12 h, mice and rabbits were injected with LPS at doses of 400 μg/kg (i.p.) or 10 μg/kg (i.v.), respectively. TNF-α activity in serum was measured at 2 h postinjection of LPS in both species. The levels of TNF-α due to LPS alone were calculated from previous time course studies with the same mice and rabbits. Injection of TSST-1 alone did not induce detectable levels of TNF-α in serum in control animals. The mean peak TNF-α level induced by TSST-1 plus LPS in mice did not significantly differ from that measured in rabbits.

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Comparison, Injection, Activity Assay, Control