Journal: Cell metabolism

Article Title: CD38-NAD + Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response

doi: 10.1016/j.cmet.2017.10.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mycoplasma testing was done in Mehrotra lab. N/A Experimental Models: Organisms/Strains C57BL/6 Jackson Laboratory Stock# 000664 C57BL/6-Tg(HLA-A2.1)1Enge/J Jackson Laboratory Stock# 003475 B6.129S7- Rag1 tm1Mom /J Jackson Laboratory Stock# 002216 B6.129P2- Cd38 tm1Lnd /J Jackson Laboratory Stock# 003727 B6;129- Sirt1 tm1Ygu /J Jackson Laboratory Stock# 008041 Tg(Cd4-cre)1Cwi/BfluJ Jackson Laboratory Stock# 017336 B6.SJL- Ptprc a Pepc b /BoyJ Jackson Laboratory Stock# 002014 B6.129S7- Ifng tm1Ts /J Jackson Laboratory Stock# 002287 B6.Cg- Rag1 tm1Mom Tyrp1 B-w Tg(Tcra,Tcrb)9Rest/J Jackson Laboratory Stock# 008684 B6.Cg- Thy1 a /Cy Tg(TcraTcrb)8Rest/J Jackson Laboratory Stock# 005023 C57BL/6-Tg(TRAMP)8247Ng/J Jackson Laboratory Stock# 003135 IFNγ Thy1.1 knockin Casey T. Weaver, University of Alabama at Birmingham (UAB) ( Harrington et al., 2008 ) Foxo1 fl/fl Lck cre Melanie Gubbels Bupp, Randolph-Macon College, VA ( Gubbels Bupp et al., 2009) TCR-I Jennifer Wu, MUSC ( Staveley-O’Carroll et al., 2003) Oligonucleotides Primers for qPCR, see Table S4 Recombinant DNA MSGV1-TRP-1 vector ( Kerkar et al., 2011 ) N/A LV-TCR/sr39TK-GFP vector ( Koya et al., 2010 ) N/A Software and Algorithms FlowJo 10.2 TreeStar, OR https://www.flowjo.com/solutions/flowjo/downloads/ Prism 5 GraphPad https://www.graphpad.com/scientiflc-software/prism/ Agilent Seahorse Wave 2.4 Agilent http://www.agilent.com/en-us/products/cell-analysis-(seahorse )/seahorse-wave-software CFX Manager 3.1 Biorad http://www.bio-rad.com/en-us/sku/soft-cfx-31-patch-cfx-manager-software-v3-1-upgrade GenomeStudio software Illumina https://www.illumina.com/techniques/microarrays/array-data-analysis-experimental-design/genomestudio.html PIMENTo ( Sásik et al., 2004 ) https://github.com/MUSC-CGM/PIMENTo ToppGene Suite ( Chen et al., 2009 ) https://toppgene.cchmc.org/ Venny This paper http://bioinfogp.cnb.csic.es/tools/venny/index.html Open in a separate window KEY RESOURCES TABLE Th1/17 cells with effector and stemness features exhibit durable tumor control High glutaminolysis of Th1/17 cell regulates its viability and anti-tumor response NAD + -Sirt1-Foxo1 axis is central to the anti-tumor phenotype of Th1/17 cells Targeting NADase CD38 on T cells increases NAD + levels and controls tumor growth

Techniques: Recombinant, Staining, Cell Isolation, Synthesized, Lysis, Extraction, Cell Based Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Immunoprecipitation, cDNA Synthesis, SYBR Green Assay, Microarray, Knock-In, Plasmid Preparation, Software

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Dose response of LPS-induced lethality and TNF-α activity in serum in rabbits primed with TSST-1

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Activity Assay

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Time course of LPS-induced TNF-α in serum in Dutch belted rabbits primed with TSST-1. Groups of three rabbits were injected with 10.0 ng (i.v.) of TSST-1 (primed)/kg or an equivalent volume of PBS (unprimed). All rabbits were injected with LPS (10 μg/kg [i.v.]) 12 h later. Control rabbits injected with 5 μg of TSST-1 and PBS/kg 12 h later did not develop detectable levels of TNF-α. (∗, P ≤ 0.05).

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Injection, Control

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Direct comparison of LPS-induced TNF-α in serum in mice and rabbits primed with TSST-1. Groups of three BALB/c-AnNCr mice and three Dutch belted rabbits were injected with TSST-1 at doses of 200 μg/kg (i.p.) or 10.0 ng/kg (i.v.), respectively. After 12 h, mice and rabbits were injected with LPS at doses of 400 μg/kg (i.p.) or 10 μg/kg (i.v.), respectively. TNF-α activity in serum was measured at 2 h postinjection of LPS in both species. The levels of TNF-α due to LPS alone were calculated from previous time course studies with the same mice and rabbits. Injection of TSST-1 alone did not induce detectable levels of TNF-α in serum in control animals. The mean peak TNF-α level induced by TSST-1 plus LPS in mice did not significantly differ from that measured in rabbits.

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Comparison, Injection, Activity Assay, Control

Journal: Cell death and differentiation

Article Title: E2F1-regulated miR-30b suppresses Cyclophilin D and protects heart from ischemia/reperfusion injury and necrotic cell death.

doi: 10.1038/cdd.2014.165

Figure Lengend Snippet: Figure 6 E2F1 promotes necrosis through miR-30b and CypD. (a) E2F1 levels are increased in cardiomyocytes exposed to H2O2. Cardiomyocytes were exposed to H2O2. Cells were harvested at the indicated times for the analysis of E2F1 levels by immunoblot. (b) Knockdown of E2F1 reduces necrotic cell death induced by H2O2. Cardiomyocytes were infected with adenoviral E2F1-siRNA or E2F1-sc. Twenty-four hours after infection, cells were treated with H2O2. PI exclusion was analyzed. *Po0.05 versus H2O2 alone. (c) The levels of E2F1 are increased in myocardial I/R. Mice were induced to undergo cardiac I/R at the indicated times as described in Materials and Methods. E2F1 levels were analyzed by immunoblot. (d and e) E2F1 knockout mice attenuates myocyte necrosis and myocardial infarction upon I/R. WTand E2F1 knockout mice were subjected to I/R as described in Materials and Methods. Myocyte necrosis (d) and myocardial infarction (e) were analyzed. *Po0.05 versus WT+I/R. (f and g) CypD TP attenuates the inhibitory effect of E2F1 knockdown on CypD expression and necrotic responses induced by H2O2. Cardiomyocytes were infected with adenoviral E2F1-siRNA or E2F1-sc, transfected with the TP (CypD-TP miR-30b) or the control (CypD-TP control) and then exposed to H2O2. CypD expression (f) was analyzed by immunoblot. Necrosis was assessed by PI exclusion assay (g). *Po0.05. GAPDH, glyceraldehyde 3-phosphate dehydrogenase

Article Snippet: Mouse E2F1 cDNA was from Origene (Rockville, MD, USA).

Techniques: Western Blot, Knockdown, Infection, Knock-Out, Expressing, Transfection, Control, Exclusion Assay