Journal: Nature cancer

Article Title: Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer

doi: 10.1038/s43018-020-00148-7

Figure Lengend Snippet: Antibodies used for CyCIF

Article Snippet: Error bars represent standard error of the mean (±SEM). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Cycle Channel Antibody Fluorophore Manufacturer Clone Catalog Number Dilution Cycle1 FITC anti-Rabbit-488 Alexa Fluor 488 ThermoFisher Polyclonal A-21246 1:2000 Cy3 anti-Rat-555 Alexa Fluor 555 ThermoFisher Polyclonal A-21434 1:2000 Cy5 anti-Mouse-647 Alexa Fluor 647 ThermoFisher Polyclonal A-21237 1:2000 Cycle 2 FITC anti-GZMB anti-Rabbit-488 Abcam Polyclonal AB4059 1:500 Cy3 anti-GZMB anti-Rat-555 eBioscience 496B 14–8889-80 1:250 Cy5 anti-GZMB anti-Mouse-647 Dako GrB-7 M7235 1:200 Cycle 3 FITC CD8a-488 Alexa Fluor 488 eBioscience AMC908 53–0008-80 1:400 Cy3 CD3D-555 Alexa Fluor 555 Abcam EP4426 {"type":"entrez-nucleotide","attrs":{"text":"AB208514","term_id":"84781018","term_text":"AB208514"}} AB208514 1:200 Cy5 CD45–647 Alexa Fluor 647 BioLegend HI30 304020 1:200 Cycle 4 FITC CD68–488 Alexa Fluor 488 CST D4B9C 24850S 1:200 Cy3 Foxp3–570 eFluor 570 eBioscience 236A/E7 41–4777-82 1:150 Cy5 CD14–647 Alexa Fluor 647 Abcam Polyclonal ab196169 1:400 Cycle 5 FITC CD163–488 Alexa Fluor 488 Abcam {"type":"entrez-protein","attrs":{"text":"EPR14643","term_id":"523380394","term_text":"EPR14643"}} EPR14643 –36 ab218293 1:200 Cy3 Keratin-570 eFluor 570 eBioscience AE1/AE3 41–9003-82 1:400 Cy5 PD1–647 Alexa Fluor 647 Abcam EPR4877(2) ab201825 1:200 Cycle 6 FITC CD4–488 Alexa Fluor 488 R&D Systems Polyclonal FAB8165G 1:200 Cy3 Ki67–570 eFluor 570 eBioscience 20Raj1 41–5699-80 1:200 Cy5 PDL1–647 Alexa Fluor 647 CST E1L3N 15005 1:200 Open in a separate window Antibodies used for CyCIF In BRCA1 -associated TNBC, macrophages were strikingly the most abundant immune cell population in the tumor, as demonstrated by a significant enrichment of CD68 + and CD163 + cells.

Techniques:

Journal: Nature cancer

Article Title: Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer

doi: 10.1038/s43018-020-00148-7

Figure Lengend Snippet: Antibodies used for murine flow cytometry

Article Snippet: Error bars represent standard error of the mean (±SEM). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Cycle Channel Antibody Fluorophore Manufacturer Clone Catalog Number Dilution Cycle1 FITC anti-Rabbit-488 Alexa Fluor 488 ThermoFisher Polyclonal A-21246 1:2000 Cy3 anti-Rat-555 Alexa Fluor 555 ThermoFisher Polyclonal A-21434 1:2000 Cy5 anti-Mouse-647 Alexa Fluor 647 ThermoFisher Polyclonal A-21237 1:2000 Cycle 2 FITC anti-GZMB anti-Rabbit-488 Abcam Polyclonal AB4059 1:500 Cy3 anti-GZMB anti-Rat-555 eBioscience 496B 14–8889-80 1:250 Cy5 anti-GZMB anti-Mouse-647 Dako GrB-7 M7235 1:200 Cycle 3 FITC CD8a-488 Alexa Fluor 488 eBioscience AMC908 53–0008-80 1:400 Cy3 CD3D-555 Alexa Fluor 555 Abcam EP4426 {"type":"entrez-nucleotide","attrs":{"text":"AB208514","term_id":"84781018","term_text":"AB208514"}} AB208514 1:200 Cy5 CD45–647 Alexa Fluor 647 BioLegend HI30 304020 1:200 Cycle 4 FITC CD68–488 Alexa Fluor 488 CST D4B9C 24850S 1:200 Cy3 Foxp3–570 eFluor 570 eBioscience 236A/E7 41–4777-82 1:150 Cy5 CD14–647 Alexa Fluor 647 Abcam Polyclonal ab196169 1:400 Cycle 5 FITC CD163–488 Alexa Fluor 488 Abcam {"type":"entrez-protein","attrs":{"text":"EPR14643","term_id":"523380394","term_text":"EPR14643"}} EPR14643 –36 ab218293 1:200 Cy3 Keratin-570 eFluor 570 eBioscience AE1/AE3 41–9003-82 1:400 Cy5 PD1–647 Alexa Fluor 647 Abcam EPR4877(2) ab201825 1:200 Cycle 6 FITC CD4–488 Alexa Fluor 488 R&D Systems Polyclonal FAB8165G 1:200 Cy3 Ki67–570 eFluor 570 eBioscience 20Raj1 41–5699-80 1:200 Cy5 PDL1–647 Alexa Fluor 647 CST E1L3N 15005 1:200 Open in a separate window Antibodies used for CyCIF In BRCA1 -associated TNBC, macrophages were strikingly the most abundant immune cell population in the tumor, as demonstrated by a significant enrichment of CD68 + and CD163 + cells.

Techniques:

Journal: Nature cancer

Article Title: Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer

doi: 10.1038/s43018-020-00148-7

Figure Lengend Snippet: Antibodies used for human flow cytometry

Article Snippet: Error bars represent standard error of the mean (±SEM). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Cycle Channel Antibody Fluorophore Manufacturer Clone Catalog Number Dilution Cycle1 FITC anti-Rabbit-488 Alexa Fluor 488 ThermoFisher Polyclonal A-21246 1:2000 Cy3 anti-Rat-555 Alexa Fluor 555 ThermoFisher Polyclonal A-21434 1:2000 Cy5 anti-Mouse-647 Alexa Fluor 647 ThermoFisher Polyclonal A-21237 1:2000 Cycle 2 FITC anti-GZMB anti-Rabbit-488 Abcam Polyclonal AB4059 1:500 Cy3 anti-GZMB anti-Rat-555 eBioscience 496B 14–8889-80 1:250 Cy5 anti-GZMB anti-Mouse-647 Dako GrB-7 M7235 1:200 Cycle 3 FITC CD8a-488 Alexa Fluor 488 eBioscience AMC908 53–0008-80 1:400 Cy3 CD3D-555 Alexa Fluor 555 Abcam EP4426 {"type":"entrez-nucleotide","attrs":{"text":"AB208514","term_id":"84781018","term_text":"AB208514"}} AB208514 1:200 Cy5 CD45–647 Alexa Fluor 647 BioLegend HI30 304020 1:200 Cycle 4 FITC CD68–488 Alexa Fluor 488 CST D4B9C 24850S 1:200 Cy3 Foxp3–570 eFluor 570 eBioscience 236A/E7 41–4777-82 1:150 Cy5 CD14–647 Alexa Fluor 647 Abcam Polyclonal ab196169 1:400 Cycle 5 FITC CD163–488 Alexa Fluor 488 Abcam {"type":"entrez-protein","attrs":{"text":"EPR14643","term_id":"523380394","term_text":"EPR14643"}} EPR14643 –36 ab218293 1:200 Cy3 Keratin-570 eFluor 570 eBioscience AE1/AE3 41–9003-82 1:400 Cy5 PD1–647 Alexa Fluor 647 Abcam EPR4877(2) ab201825 1:200 Cycle 6 FITC CD4–488 Alexa Fluor 488 R&D Systems Polyclonal FAB8165G 1:200 Cy3 Ki67–570 eFluor 570 eBioscience 20Raj1 41–5699-80 1:200 Cy5 PDL1–647 Alexa Fluor 647 CST E1L3N 15005 1:200 Open in a separate window Antibodies used for CyCIF In BRCA1 -associated TNBC, macrophages were strikingly the most abundant immune cell population in the tumor, as demonstrated by a significant enrichment of CD68 + and CD163 + cells.

Techniques:

Journal: Toxicological Sciences

Article Title: Generation of a New Model Rat: Nrf2 Knockout Rats Are Sensitive to Aflatoxin B 1 Toxicity

doi: 10.1093/toxsci/kfw065

Figure Lengend Snippet: Antibodies Used in Western Blot

Article Snippet: The antibodies used for Western blot are listed in . table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Antibody Catalog Number Reference or Company Anti-AKR7A2 ab175295 Abcam PLC, Cambridge, United Kingdom Anti-AKR7A3 13209-1-AP Proteintech Group, Inc., Chicago Anti-GSTA3 Mclellan et al. (1994) Anti-Keap1 No. 111 Watai et al. (2007) Anti-Lamin B sc-6217 Santa Cruz Biotechnology Inc., Dallas, Texas Anti-Nrf2 No. 103 Maruyama et al. (2008) Anti-NQO1 ab2346 Abcam PLC, Cambridge, United Kingdom Anti-αTubulin T9026 Sigma-Aldrich Co., LLC, St Louis Open in a separate window Antibodies Used in Western Blot

Techniques: Western Blot