Journal: Ecotoxicology and environmental safety

Article Title: Ameliorative effect of betulinic acid against zearalenone exposure triggers testicular dysfunction and oxidative stress in mice via p38/ERK MAPK inhibition and Nrf2-mediated antioxidant defense activation.

doi: 10.1016/j.ecoenv.2022.113561

Figure Lengend Snippet: Fig. 2. BA protected the aggravation of male reproduction injury in ZEA-induced mice. The morphology of the sperm was photographed using an optical microscope (A). The sperm motility was used to evaluate the male repro duction of mice, including sperm survival rate (B), sperm malformation rate (C), and sperm mortality rate (D). Protein and mRNA levels of ERα in testis were detected by immunoblotting and RT-PCR analysis, respectively, and the pro tein and mRNA levels were normalized to β-actin (E-G). The content of testosterone in serum was detected by ELISA kit (H). The mRNA expression of CLDN11 (I), CDH2 (J), and Vim (K) were measured by RT-PCR. Mean ± SEM, *P < 0.05 and **P < 0.01 represented a significant differ ence compared to the control group, while #P < 0.05 and ##P < 0.01 represented a signifi cant difference compared to the ZEA group.

Article Snippet: Testosterone enzyme linked immunosorbent assay (ELISA) kit (CSB-E05101m) was obtained from Cusabio Biotech Co. Ltd. (Wuhan, China).

Techniques: Microscopy, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Expressing, Control

Journal: Translational Andrology and Urology

Article Title: Prostatic artery occlusion: initial findings on pathophysiological response in a canine prostate model

doi: 10.21037/tau-22-423

Figure Lengend Snippet: Data of intraprostatic hormone concentrations and apoptosis induced by PAO compared with control at 6-month of follow-up

Article Snippet: Canine testosterone was measured using a solid phase sandwich ELISA method [Canine Testosterone (T) ELISAN Kit, CUSABIO, Wuhan Hi-tech Medical Devices Park, Wuhan, Hubei Province, China].

Techniques: Control, TUNEL Assay

Journal: Theriogenology

Article Title: Biostimulation methods based on chemical communication improve semen quality in male breeder rabbits.

doi: 10.1016/j.theriogenology.2024.09.016

Figure Lengend Snippet: Fig. 3. Graphical representation of the mean testosterone concentration (ng/mL) determined by ELISA analysis in male breeder rabbit serum samples, collected from 24 male rabbits before (T1) and during (T2, T3, T4, T5) exposure to female urine, 2-phenoxyethanol, vaginal swab and distilled water (control). T1: 150 min; T2: 90 min; T3: 60 min; T4: 30 min; T5: 2 min before semen collection.

Article Snippet: Testosterone concentration was determined using a Rabbit Testosterone ELISA Kit (Cusabio Technology LLC, Houston, TX, USA), following the manufacturer’s instructions.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control