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Image Search Results
Journal: BMC Complementary Medicine and Therapies
Article Title: Tocotrienol-rich fraction (TRF) protects against retinal cell apoptosis and preserves visual behavior in rats with streptozotocin-induced diabetic retinopathy
doi: 10.1186/s12906-024-04614-y
Figure Lengend Snippet: A . Microphotographs of retinal sections showing the effect of TRF on diabetes-induced retinal cell apoptosis as detected using TUNEL staining (20× magnification). Scale bar represents 100 μm. Yellow arrow: TUNEL positive cells; Left panel: DAPI; Middle panel: FITC; Right panel: Merged (DAPI and FITC). B . Quantitative estimation of the retinal cell apoptosis in the ganglion cell layer showing the effect of TRF in diabetic rats. Bars represent mean ± SD. n = 6, *** p < 0.001. ANOVA and the post hoc Tukey test were used for statistical comparison among groups. N: Rats that received the vehicle, DV: Diabetic rats that received the vehicle, DT: TRF treated diabetic rats
Article Snippet: The detection of apoptotic cells in retinal tissue was carried out using the
Techniques: TUNEL Assay, Staining, Comparison
Journal: BioMed Research International
Article Title: Excess Hydrocortisone Hampers Placental Nutrient Uptake Disrupting Cellular Metabolism
doi: 10.1155/2018/5106174
Figure Lengend Snippet: Assessment of hydrocortisone-induced cellular death by apoptosis in healthy-term placental explants. (a) Representative microphotographs of TUNEL assay to determine apoptosis in each experimental condition; cells undergoing apoptosis present fluorescent green nuclei. (b) Quantification of the % of TUNEL positive cells per condition. (c) Active caspases 3 and 7 as measured by luminometry, using the Caspase Glo Assay. (d) Cleaved caspase 3 as determined by western blot and normalized by actin levels. Values are presented as means ± SEM for 5 independent experiments. ∗ p≤0.05; ∗∗ p≤0.01; ∗∗∗ p≤0.001 relative to control.
Article Snippet: Apoptosis was assessed with a commercially available
Techniques: TUNEL Assay, Caspase-Glo Assay, Western Blot, Control