Journal: Heliyon

Article Title: Jian-Pi-Yin decoction attenuates lactose-induced chronic diarrhea in rats by regulating GLP-1 and reducing NHE3 ubiquitination and phosphorylation

doi: 10.1016/j.heliyon.2023.e17444

Figure Lengend Snippet: Effect of JPY on the expression of NHE3 in the colon and jejunum of rats. (A-B) Analysis of the WB bands of NHE3 of colon and jejunum. (C-D) Analysis of mRNA expressions of NHE3 of colon and jejunum. (E-F) Analysis of IHC staining of NHE3 of colon and jejunum. (G) Representative photos of NHE3 of the colon and jejunum with IHC staining. (H-I) Comparison of the WB bands, mRNA expressions, and IHC staining of colon and jejunum NHE3. (K) Representative WB bands of colon and jejunum NHE3. (S4KC) Supplementary material of uncropped versions of colon NHE3. (S4KJ) Supplementary material of uncropped versions of jejunum NHE3. Data were presented as mean ± SEM (n = 3). * P < 0.05 compared with the control group and # P < 0.05 compared with the model group. Magnification at 200×.

Article Snippet: NHE3 inhibitor Tenapanor (GC19352) was purchased from GlpBio (Mo, Ca, USA).

Techniques: Expressing, Immunohistochemistry, Comparison, Control

Journal: Heliyon

Article Title: Jian-Pi-Yin decoction attenuates lactose-induced chronic diarrhea in rats by regulating GLP-1 and reducing NHE3 ubiquitination and phosphorylation

doi: 10.1016/j.heliyon.2023.e17444

Figure Lengend Snippet: Effect of JPY on the expression of phosphorylated NHE3 in the colon and jejunum of rats. (A-B) Analysis of the WB bands of phosphorylated NHE3 of colon and jejunum. (C-D) Analysis of IHC staining of phosphorylated NHE3 of colon and jejunum. (G) Representative photos of phosphorylated NHE3 of the colon and jejunum with IHC staining. (H) Representative WB bands of colon and jejunum phosphorylated NHE3. (E-F) Analysis of the WB bands of phosphorylated NHE3/NHE3 of the colon and jejunum. (I-J) Analysis of the IHC staining of phosphorylated NHE3/NHE3 of the colon and jejunum. (K, L) Comparison of the WB bands and IHC staining of colon and jejunum phosphorylated NHE3/NHE3. (S7HC) Supplementary material of uncropped versions of colon phosphorylated NHE3/NHE3. (S7HJ) Supplementary material of uncropped versions of jejunum phosphorylated NHE3/NHE3. Data were presented as mean ± SEM (n = 3). * P < 0.05 compared with the control group and # P < 0.05 compared with the model group. Magnification at 200×.

Article Snippet: NHE3 inhibitor Tenapanor (GC19352) was purchased from GlpBio (Mo, Ca, USA).

Techniques: Expressing, Immunohistochemistry, Comparison, Control

Journal: Heliyon

Article Title: Jian-Pi-Yin decoction attenuates lactose-induced chronic diarrhea in rats by regulating GLP-1 and reducing NHE3 ubiquitination and phosphorylation

doi: 10.1016/j.heliyon.2023.e17444

Figure Lengend Snippet: Effect of JPY on the expression of ubiquitinated NHE3 in the colon and jejunum of rats. (A-B) Analysis of the WB bands of ubiquitinated NHE3 of colon and jejunum. (C-D) Analysis of mRNA expressions of ubiquitinated NHE3 of colon and jejunum. (E-F) Analysis of IHC staining of ubiquitinated NHE3 of colon and jejunum. (G) Representative photos of ubiquitinated NHE3 of the colon and jejunum with IHC staining. (H-I) Comparison of the WB bands, mRNA expressions and IHC staining of colon and jejunum ubiquitinated NHE3/NHE3. (K) Representative WB bands of colon and jejunum ubiquitinated NHE3. (L-M) Analysis of the WB bands of ubiquitinated NHE3/NHE3 of the colon and jejunum. (N-O) Analysis of the IHC staining of ubiquitinated NHE3/NHE3 of the colon and jejunum. (S8KC) Supplementary material of uncropped versions of colon ubiquitinated NHE3/NHE3. (S8KJ) Supplementary material of uncropped versions of jejunum ubiquitinated NHE3/NHE3. Data were presented as mean ± SEM (n = 3). * P < 0.05 compared with the control group and # P < 0.05 compared with the model group. Magnification at 200×.

Article Snippet: NHE3 inhibitor Tenapanor (GC19352) was purchased from GlpBio (Mo, Ca, USA).

Techniques: Expressing, Immunohistochemistry, Comparison, Control

Journal: Heliyon

Article Title: Jian-Pi-Yin decoction attenuates lactose-induced chronic diarrhea in rats by regulating GLP-1 and reducing NHE3 ubiquitination and phosphorylation

doi: 10.1016/j.heliyon.2023.e17444

Figure Lengend Snippet: This schematic summarizes the molecular mechanism of JPY against diarrhea in a rat model of hyper lactose-induced diarrhea. Its mechanism of action includes reducing the expression of GLP-1 and its related receptors, inhibiting the activation of the cAMP/PKA pathway, reducing the ubiquitination and phosphorylation of NHE3, and increasing NHE3 expression to restore intestinal absorption function.

Article Snippet: NHE3 inhibitor Tenapanor (GC19352) was purchased from GlpBio (Mo, Ca, USA).

Techniques: Expressing, Activation Assay, Ubiquitin Proteomics, Phospho-proteomics

Journal: Clinical and Experimental Nephrology

Article Title: A phase 1 study of the safety, tolerability, pharmacodynamics, and pharmacokinetics of tenapanor in healthy Japanese volunteers

doi: 10.1007/s10157-016-1302-8

Figure Lengend Snippet: Demographic and baseline characteristics of the Japanese participants

Article Snippet: Plasma concentrations of tenapanor were measured by MicroConstants, Inc. (San Diego, CA, USA).

Techniques:

Journal: Clinical and Experimental Nephrology

Article Title: A phase 1 study of the safety, tolerability, pharmacodynamics, and pharmacokinetics of tenapanor in healthy Japanese volunteers

doi: 10.1007/s10157-016-1302-8

Figure Lengend Snippet: Excretion of sodium via a stool and b urine in healthy Japanese volunteers treated with tenapanor or placebo. Data are shown as mean + SD; corresponding values given above the bars show mean (SD). a daily mean over 7 days. b combined data from participants receiving single ( n = 2) and repeated ( n = 12) doses (one individual in the placebo group was excluded from stool analyses owing to a lack of any post-baseline samples). SD standard deviation

Article Snippet: Plasma concentrations of tenapanor were measured by MicroConstants, Inc. (San Diego, CA, USA).

Techniques: Standard Deviation

Journal: Clinical and Experimental Nephrology

Article Title: A phase 1 study of the safety, tolerability, pharmacodynamics, and pharmacokinetics of tenapanor in healthy Japanese volunteers

doi: 10.1007/s10157-016-1302-8

Figure Lengend Snippet: Excretion of phosphorus via a stool and b urine in Japanese healthy volunteers treated with tenapanor or placebo. Data are shown as mean + SD; corresponding values given above the bars show mean (SD). a daily mean over 7 days. b combined data from participants receiving single ( n = 2) and repeated ( n = 12) doses (one individual in the placebo group was excluded from stool analyses owing to a lack of any post-baseline samples). SD standard deviation

Article Snippet: Plasma concentrations of tenapanor were measured by MicroConstants, Inc. (San Diego, CA, USA).

Techniques: Standard Deviation

Journal: Clinical and Experimental Nephrology

Article Title: A phase 1 study of the safety, tolerability, pharmacodynamics, and pharmacokinetics of tenapanor in healthy Japanese volunteers

doi: 10.1007/s10157-016-1302-8

Figure Lengend Snippet: Summary of stool frequency, weight, and consistency in healthy Japanese individuals treated with tenapanor or placebo

Article Snippet: Plasma concentrations of tenapanor were measured by MicroConstants, Inc. (San Diego, CA, USA).

Techniques:

Journal: Clinical Pharmacology in Drug Development

Article Title: Effect of Food Intake on the Pharmacodynamics of Tenapanor: A Phase 1 Study

doi: 10.1002/cpdd.341

Figure Lengend Snippet: Other Pharmacodynamic Variables

Article Snippet: Plasma tenapanor concentrations were determined by Covance Laboratories (Madison, Wisconsin).

Techniques:

Journal: American Journal of Physiology - Cell Physiology

Article Title: Reduced surface pH and upregulated AE2 anion exchange in SLC26A3-deleted polarized intestinal epithelial cells

doi: 10.1152/ajpcell.00590.2023

Figure Lengend Snippet: Lack of SLC26A3 in differentiated Caco2BBe monolayers strongly affects apical surface acid/base balance. A : schematic of the ratiometric technique to measure apical compartment pH of epithelial monolayers using pH-sensitive (pHrodo red) and pH-insensitive (AlexaFluor 488) dyes coupled to dextran. B : time course of change in apical surface pH for differentiated Caco2BBe monolayers shows much lower steady-state values for SLC26A3-KO cells than for WT. C : inhibition of NHE3 with 1 µM tenapanor and of NHE8 with 3 µM HOE642 increases monolayer apical surface pH for both SLC26A3-WT and KO, while maintaining significant differences between WT and KO values. D : Apical surface proton concentrations calculated for each condition based on pH values in C , by equation [H + ] = 10 −pH . E : NHE3/8 inhibition-dependent difference in apical surface proton concentrations for SLC26A3-WT and KO monolayers. F : summary of data from C – E . n ≥ 5, Student’s t test, ** P ≤ 0.01, *** P ≤ 0.001.

Article Snippet: Where indicated, this composition was also supplemented with a combination of 1 μM tenapanor (Adooq Bioscience, Irvine, CA) to inhibit NHE3 activity and 3 μM HOE642 (kindly provided by Sanofi-Aventis; Frankfurt, Germany) to inhibit NHE8 activity , or with 1 μM tenapanor only.

Techniques: Inhibition