te2000u Search Results


nikon te2000u microscope custom  (Nikon)
96
Nikon nikon te2000u microscope custom
Nikon Te2000u Microscope Custom, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nikon te2000u microscope custom/product/Nikon
Average 96 stars, based on 1 article reviews
nikon te2000u microscope custom - by Bioz Stars, 2026-03
96/100 stars
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inverted microscope nikon eclipse te2000u  (Nikon)
99
Nikon inverted microscope nikon eclipse te2000u
Inverted Microscope Nikon Eclipse Te2000u, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverted microscope nikon eclipse te2000u/product/Nikon
Average 99 stars, based on 1 article reviews
inverted microscope nikon eclipse te2000u - by Bioz Stars, 2026-03
99/100 stars
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widefield microscope te2000-u  (Omicron Laserage Laserprodukte GmbH)
90
Omicron Laserage Laserprodukte GmbH widefield microscope te2000-u
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Widefield Microscope Te2000 U, supplied by Omicron Laserage Laserprodukte GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/widefield microscope te2000-u/product/Omicron Laserage Laserprodukte GmbH
Average 90 stars, based on 1 article reviews
widefield microscope te2000-u - by Bioz Stars, 2026-03
90/100 stars
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fluorescence microscope te2000u  (Tecan Systems)
90
Tecan Systems fluorescence microscope te2000u
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Fluorescence Microscope Te2000u, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence microscope te2000u/product/Tecan Systems
Average 90 stars, based on 1 article reviews
fluorescence microscope te2000u - by Bioz Stars, 2026-03
90/100 stars
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microscope te-2000u  (Canon inc)
90
Canon inc microscope te-2000u
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Microscope Te 2000u, supplied by Canon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microscope te-2000u/product/Canon inc
Average 90 stars, based on 1 article reviews
microscope te-2000u - by Bioz Stars, 2026-03
90/100 stars
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inverted microscope te2000u  (Eppendorf AG)
90
Eppendorf AG inverted microscope te2000u
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Inverted Microscope Te2000u, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverted microscope te2000u/product/Eppendorf AG
Average 90 stars, based on 1 article reviews
inverted microscope te2000u - by Bioz Stars, 2026-03
90/100 stars
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fluorescence microscope eclapse te 2000-u  (Evident Corporation)
90
Evident Corporation fluorescence microscope eclapse te 2000-u
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Fluorescence Microscope Eclapse Te 2000 U, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence microscope eclapse te 2000-u/product/Evident Corporation
Average 90 stars, based on 1 article reviews
fluorescence microscope eclapse te 2000-u - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved widefield images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.

Journal: The Journal of Cell Biology

Article Title: Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis

doi: 10.1083/jcb.201009119

Figure Lengend Snippet: WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved widefield images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.

Article Snippet: For qualitative high speed acquisition, cells were imaged on a TE2000-U widefield microscope with a 100× 1.49 NA objective, with illumination provided by 473- and 561-nm solid-state lasers (Deepstar; Omicron Laserage Laserprodukte GmbH).

Techniques: Staining, Expressing

WASH causes recycling of the V-ATPase. (a) Confocal imaging of wshA − cells expressing GFP-WASH and VatB-mRFP after endocytosis of 0.5-µm agarose beads. Frames are taken from Video 3 . Insets show a magnified view of the vesicle on which WASH is acting. (b) Quantification of various vesicular proteins during postlysosome formation. Graphs show that the loss of V-ATPase immediately follows the arrival of WASH and coronin, whereas the previously described postlysosome marker vacuolin is present much earlier than neutralization and rises steadily. The representative curves are from an experiment that was performed at least five times. (c) Rapid widefield oblique illumination imaging of small vesicles containing both GFP-WASH and VatB-mRFP budding a single lysosome. Frames are taken from Video 4 . Bar, 1 µm.

Journal: The Journal of Cell Biology

Article Title: Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis

doi: 10.1083/jcb.201009119

Figure Lengend Snippet: WASH causes recycling of the V-ATPase. (a) Confocal imaging of wshA − cells expressing GFP-WASH and VatB-mRFP after endocytosis of 0.5-µm agarose beads. Frames are taken from Video 3 . Insets show a magnified view of the vesicle on which WASH is acting. (b) Quantification of various vesicular proteins during postlysosome formation. Graphs show that the loss of V-ATPase immediately follows the arrival of WASH and coronin, whereas the previously described postlysosome marker vacuolin is present much earlier than neutralization and rises steadily. The representative curves are from an experiment that was performed at least five times. (c) Rapid widefield oblique illumination imaging of small vesicles containing both GFP-WASH and VatB-mRFP budding a single lysosome. Frames are taken from Video 4 . Bar, 1 µm.

Article Snippet: For qualitative high speed acquisition, cells were imaged on a TE2000-U widefield microscope with a 100× 1.49 NA objective, with illumination provided by 473- and 561-nm solid-state lasers (Deepstar; Omicron Laserage Laserprodukte GmbH).

Techniques: Imaging, Expressing, Marker, Neutralization