Journal: Journal of Inflammation Research

Article Title: Identification of Biomarkers for Sepsis-Induced Acute Lung Injury Through Bioinformatics and Machine Learning Approaches, with Experimental Validation

doi: 10.2147/JIR.S539899

Figure Lengend Snippet: Two-and three-dimensional visualization of the binding interactions between resveratrol and four target proteins. ( A ) DDAH2, ( B ) PNPLA2, ( C ) STXBP2, ( D ) TCN1. Resveratrol is shown in red, and the target proteins are depicted in green. Binding affinities are indicated in kcal/mol.

Article Snippet: The membranes were blocked with 5% non-fat milk in TBST at room temperature for 2 hours, followed by overnight incubation at 4 °C with primary antibodies targeting DDAH2 (1:3000, 14966-1-AP, Proteintech), PNPLA2 (1:5000, 55190-1-AP, Proteintech), STXBP2 (1:1000, 15312-1-AP, Proteintech), and TCN1 (1:1000, 16078-1-AP, Proteintech).

Techniques: Binding Assay

Journal: Journal of Inflammation Research

Article Title: Identification of Biomarkers for Sepsis-Induced Acute Lung Injury Through Bioinformatics and Machine Learning Approaches, with Experimental Validation

doi: 10.2147/JIR.S539899

Figure Lengend Snippet: Resveratrol reversed the expression of target genes to alleviate LPS-induced ALI. ( A ) Cell activity of BEAS-2B cell lines treated with LPS for 24 h. ( B ) Cell activity of BEAS-2B cell lines treated with Resveratrol for 1 h. ( C ) The changes in cell morphology after LPS administration with or without pretreatment with Resveratrol at a concentration of 10 µM. ( D-G ) mRNA expression levels of DDAH2, PNPLA2, STXBP2, and TCN1 in BEAS-2B cells. ( H-L ) Expression of DDAH2, PNPLA2, STXBP2, and TCN1 proteins in BEAS-2B cells.

Article Snippet: The membranes were blocked with 5% non-fat milk in TBST at room temperature for 2 hours, followed by overnight incubation at 4 °C with primary antibodies targeting DDAH2 (1:3000, 14966-1-AP, Proteintech), PNPLA2 (1:5000, 55190-1-AP, Proteintech), STXBP2 (1:1000, 15312-1-AP, Proteintech), and TCN1 (1:1000, 16078-1-AP, Proteintech).

Techniques: Expressing, Activity Assay, Concentration Assay