tcf11 nrf1 Search Results


antibody targeting nrf1 tcf11  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc antibody targeting nrf1 tcf11
Antibody Targeting Nrf1 Tcf11, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcf11+nrf1/TCF11%2FNRF1+Rabbit+mAb/pmc12574707-368-7-11
Average 95 stars, based on 1 article reviews
antibody targeting nrf1 tcf11 - by Bioz Stars, 2026-10
95/100 stars
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antibodies against nrf1  (Proteintech)
92
Proteintech antibodies against nrf1
Detection of endogenous <t>NRF1-742</t> and NRF1-772 proteins and their nuclear accumulation. ( A ) Schematic diagram of different isoforms of the human NRF1 transcripts. Green and white open boxes represent the coding and untranslated regions, respectively. The solid black lines represent the introns. The sequences are from the National Center for Biotechnology ( www.ncbi.hlm.nih.gov ) and Ensemble Genome Browser ( www.ensemble.org ), updated as of November 2019. ( B ) NRF1 protein expression in HaCaT cells exposed to iAs 3+ or vehicle (medium) for 6 h. Whole-cell lysates (WCL), cytosolic fractions (CF), and nuclear fractions (NF) from Cont -overexpressing (OE) ( C ), NRF1-742 -OE ( D ), and NRF1-772 -OE cells ( E ) following treatment with vehicle (medium) or 10 μM iAs 3+ for 6 h. To better visualize the weak bands, the films with weak bands (red boxes) were cut for another longer exposure following the initial exposure of the whole membrane (labeled as A2, E2).
Antibodies Against Nrf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcf11+nrf1/NFE2L1+Antibody/pmc07139366-197-1-24
Average 92 stars, based on 1 article reviews
antibodies against nrf1 - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier
anti nfe2l1 antibody a14753  (ABclonal Biotechnology)
94
ABclonal Biotechnology anti nfe2l1 antibody a14753
Detection of endogenous <t>NRF1-742</t> and NRF1-772 proteins and their nuclear accumulation. ( A ) Schematic diagram of different isoforms of the human NRF1 transcripts. Green and white open boxes represent the coding and untranslated regions, respectively. The solid black lines represent the introns. The sequences are from the National Center for Biotechnology ( www.ncbi.hlm.nih.gov ) and Ensemble Genome Browser ( www.ensemble.org ), updated as of November 2019. ( B ) NRF1 protein expression in HaCaT cells exposed to iAs 3+ or vehicle (medium) for 6 h. Whole-cell lysates (WCL), cytosolic fractions (CF), and nuclear fractions (NF) from Cont -overexpressing (OE) ( C ), NRF1-742 -OE ( D ), and NRF1-772 -OE cells ( E ) following treatment with vehicle (medium) or 10 μM iAs 3+ for 6 h. To better visualize the weak bands, the films with weak bands (red boxes) were cut for another longer exposure following the initial exposure of the whole membrane (labeled as A2, E2).
Anti Nfe2l1 Antibody A14753, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcf11+nrf1/NFE2L1+Rabbit+pAb/pm42086188-57-1-11
Average 94 stars, based on 1 article reviews
anti nfe2l1 antibody a14753 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
anti-tcf11/nrf1 antibody  (St Johns Laboratory)
N/A
Mouse monoclonal to TCF11/NRF1. Conjugation note: Unconjugated Application note: WB, ELISA Reactivity note: Human
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Image Search Results


Detection of endogenous NRF1-742 and NRF1-772 proteins and their nuclear accumulation. ( A ) Schematic diagram of different isoforms of the human NRF1 transcripts. Green and white open boxes represent the coding and untranslated regions, respectively. The solid black lines represent the introns. The sequences are from the National Center for Biotechnology ( www.ncbi.hlm.nih.gov ) and Ensemble Genome Browser ( www.ensemble.org ), updated as of November 2019. ( B ) NRF1 protein expression in HaCaT cells exposed to iAs 3+ or vehicle (medium) for 6 h. Whole-cell lysates (WCL), cytosolic fractions (CF), and nuclear fractions (NF) from Cont -overexpressing (OE) ( C ), NRF1-742 -OE ( D ), and NRF1-772 -OE cells ( E ) following treatment with vehicle (medium) or 10 μM iAs 3+ for 6 h. To better visualize the weak bands, the films with weak bands (red boxes) were cut for another longer exposure following the initial exposure of the whole membrane (labeled as A2, E2).

Journal: International Journal of Molecular Sciences

Article Title: Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes

doi: 10.3390/ijms21062014

Figure Lengend Snippet: Detection of endogenous NRF1-742 and NRF1-772 proteins and their nuclear accumulation. ( A ) Schematic diagram of different isoforms of the human NRF1 transcripts. Green and white open boxes represent the coding and untranslated regions, respectively. The solid black lines represent the introns. The sequences are from the National Center for Biotechnology ( www.ncbi.hlm.nih.gov ) and Ensemble Genome Browser ( www.ensemble.org ), updated as of November 2019. ( B ) NRF1 protein expression in HaCaT cells exposed to iAs 3+ or vehicle (medium) for 6 h. Whole-cell lysates (WCL), cytosolic fractions (CF), and nuclear fractions (NF) from Cont -overexpressing (OE) ( C ), NRF1-742 -OE ( D ), and NRF1-772 -OE cells ( E ) following treatment with vehicle (medium) or 10 μM iAs 3+ for 6 h. To better visualize the weak bands, the films with weak bands (red boxes) were cut for another longer exposure following the initial exposure of the whole membrane (labeled as A2, E2).

Article Snippet: The antibodies against NRF1 (17062-1-AP), NQO1 (11451-1-AP), GCLC (12601-1-AP), KEAP1 (10503-2-AP), GCLM (14241-1-AP), tubulin (10094-1-AP), lamin A/C (10298-1-AP), and β-actin (60008-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques: Expressing, Membrane, Labeling

Subcellular localization of NRF1-742 and NRF1-772 proteins and their derivative bands under normal or iAs 3+ treatment conditions.

Journal: International Journal of Molecular Sciences

Article Title: Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes

doi: 10.3390/ijms21062014

Figure Lengend Snippet: Subcellular localization of NRF1-742 and NRF1-772 proteins and their derivative bands under normal or iAs 3+ treatment conditions.

Article Snippet: The antibodies against NRF1 (17062-1-AP), NQO1 (11451-1-AP), GCLC (12601-1-AP), KEAP1 (10503-2-AP), GCLM (14241-1-AP), tubulin (10094-1-AP), lamin A/C (10298-1-AP), and β-actin (60008-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques:

Effects of ER stressors and deglycosylase on NRF1 migration. Immunoblotting of NRF1 in whole cell lysates from Cont -OE ( A – C ), NRF1-742 -OE ( D – F ), or NRF1-772 -OE ( G – I ) cells 6 h post-treatment with the indicated agents. The doses of the agents were as follows: vehicle (0.5% DMSO), 10 μM iAs 3+ , 2 μg/mL Tunicamycin (TU), 1 μg/mL Brefeldin A (BFA), or 2 μM Thapsigargin (TG). ( A , D , G ) Samples were treated with Endo H (1000 U) at 37 °C for 1 h. ( B , E , H ) PNGase F-catalyzed deglycosylation (500 U) was performed for 1 h at 37 °C. Immunoblotting was performed with NRF1 and β-actin antibodies. Quantification of the NRF1 bands after deglycosylase treatment is shown in the .

Journal: International Journal of Molecular Sciences

Article Title: Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes

doi: 10.3390/ijms21062014

Figure Lengend Snippet: Effects of ER stressors and deglycosylase on NRF1 migration. Immunoblotting of NRF1 in whole cell lysates from Cont -OE ( A – C ), NRF1-742 -OE ( D – F ), or NRF1-772 -OE ( G – I ) cells 6 h post-treatment with the indicated agents. The doses of the agents were as follows: vehicle (0.5% DMSO), 10 μM iAs 3+ , 2 μg/mL Tunicamycin (TU), 1 μg/mL Brefeldin A (BFA), or 2 μM Thapsigargin (TG). ( A , D , G ) Samples were treated with Endo H (1000 U) at 37 °C for 1 h. ( B , E , H ) PNGase F-catalyzed deglycosylation (500 U) was performed for 1 h at 37 °C. Immunoblotting was performed with NRF1 and β-actin antibodies. Quantification of the NRF1 bands after deglycosylase treatment is shown in the .

Article Snippet: The antibodies against NRF1 (17062-1-AP), NQO1 (11451-1-AP), GCLC (12601-1-AP), KEAP1 (10503-2-AP), GCLM (14241-1-AP), tubulin (10094-1-AP), lamin A/C (10298-1-AP), and β-actin (60008-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques: Migration, Western Blot

NRF1-742 -OE and NRF1-772 -OE HaCaT cells are resistant to the iAs 3+ -induced cytotoxicity. ( A ) Cell viability was assessed by the MTS assay 24 h of post- iAs 3+ exposure ( n = 6). The data are presented as the mean ± SD; * p < 0.05, NRF1-742 -OE versus Cont -OE HaCaT cells; # p < 0.05, NRF1-772 -OE cells versus Cont -OE HaCaT cells. ( B ) Representative flow cytometry images of Annexin Ⅴ and PI staining. ( C ) Quantitative analysis of apoptosis. Cells were treated with the indicated concentrations of iAs 3+ for 18 h. The data are presented as the mean ± SD; * p < 0.05, NRF1-742 -OE versus Cont -OE HaCaT cells; # p < 0.05, NRF1-772 -OE cells versus Cont -OE HaCaT cells. ( D , E ) Cleaved PARP and caspase-3 immunoblotting of whole cell lysates from HaCaT cells treated with 30 μM iAs 3+ for 6 h. β-actin served as the loading control.

Journal: International Journal of Molecular Sciences

Article Title: Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes

doi: 10.3390/ijms21062014

Figure Lengend Snippet: NRF1-742 -OE and NRF1-772 -OE HaCaT cells are resistant to the iAs 3+ -induced cytotoxicity. ( A ) Cell viability was assessed by the MTS assay 24 h of post- iAs 3+ exposure ( n = 6). The data are presented as the mean ± SD; * p < 0.05, NRF1-742 -OE versus Cont -OE HaCaT cells; # p < 0.05, NRF1-772 -OE cells versus Cont -OE HaCaT cells. ( B ) Representative flow cytometry images of Annexin Ⅴ and PI staining. ( C ) Quantitative analysis of apoptosis. Cells were treated with the indicated concentrations of iAs 3+ for 18 h. The data are presented as the mean ± SD; * p < 0.05, NRF1-742 -OE versus Cont -OE HaCaT cells; # p < 0.05, NRF1-772 -OE cells versus Cont -OE HaCaT cells. ( D , E ) Cleaved PARP and caspase-3 immunoblotting of whole cell lysates from HaCaT cells treated with 30 μM iAs 3+ for 6 h. β-actin served as the loading control.

Article Snippet: The antibodies against NRF1 (17062-1-AP), NQO1 (11451-1-AP), GCLC (12601-1-AP), KEAP1 (10503-2-AP), GCLM (14241-1-AP), tubulin (10094-1-AP), lamin A/C (10298-1-AP), and β-actin (60008-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques: MTS Assay, Flow Cytometry, Staining, Western Blot, Control

Crosstalk between NRF1, nuclear factor erythroid 2-like 2 (NRF2), and Kelch-like ECH- associated protein 1 (KEAP1). Cells were exposed to 10 μM iAs 3+ or vehicle for 6 h. Immunoblotting of NRF2 in whole-cell lysates ( A ) and nuclear cell fractions ( C ). Quantification of NRF2 bands in whole-cell lysates ( B ) and the nuclear fractions ( D ). (E-F) RT-qPCR analysis of NRF2 and KEAP1 expression in response to acute iAs 3+ exposure ( n = 6). The data are presented as the mean ± SD; * p < 0.05, NRF1-742 -OE versus Cont -OE HaCaT cells; # p < 0.05, NRF1-772 -OE cells versus Cont -OE HaCaT cells.

Journal: International Journal of Molecular Sciences

Article Title: Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes

doi: 10.3390/ijms21062014

Figure Lengend Snippet: Crosstalk between NRF1, nuclear factor erythroid 2-like 2 (NRF2), and Kelch-like ECH- associated protein 1 (KEAP1). Cells were exposed to 10 μM iAs 3+ or vehicle for 6 h. Immunoblotting of NRF2 in whole-cell lysates ( A ) and nuclear cell fractions ( C ). Quantification of NRF2 bands in whole-cell lysates ( B ) and the nuclear fractions ( D ). (E-F) RT-qPCR analysis of NRF2 and KEAP1 expression in response to acute iAs 3+ exposure ( n = 6). The data are presented as the mean ± SD; * p < 0.05, NRF1-742 -OE versus Cont -OE HaCaT cells; # p < 0.05, NRF1-772 -OE cells versus Cont -OE HaCaT cells.

Article Snippet: The antibodies against NRF1 (17062-1-AP), NQO1 (11451-1-AP), GCLC (12601-1-AP), KEAP1 (10503-2-AP), GCLM (14241-1-AP), tubulin (10094-1-AP), lamin A/C (10298-1-AP), and β-actin (60008-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques: Western Blot, Quantitative RT-PCR, Expressing

Expression of ARE-antioxidant genes, PSMC3 , PSMC4 , and XPC . ( A ) Antioxidant protein expression (GCLC, GCLM, and NQO1) was measured by immunoblotting after treatment with 10 μM iAs 3+ or vehicle for 6 h. ( B – D ) Quantification of GCLC, GCLM, and NQO1 protein levels. ( E – J ) RT-qPCR analysis of the expression of antioxidant response element (ARE)-antioxidant genes ( E – G ), PSMC3 ( H ), PSMC4 ( I ), and XPC ( J ) following vehicle or iAs 3+ treatment ( n = 6). The data are presented as the mean ± SD; * p < 0.05, NRF1-742 -OE versus Cont -OE HaCaT cells; # p < 0.05, NRF1-772 -OE versus Cont -OE HaCaT cells.

Journal: International Journal of Molecular Sciences

Article Title: Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes

doi: 10.3390/ijms21062014

Figure Lengend Snippet: Expression of ARE-antioxidant genes, PSMC3 , PSMC4 , and XPC . ( A ) Antioxidant protein expression (GCLC, GCLM, and NQO1) was measured by immunoblotting after treatment with 10 μM iAs 3+ or vehicle for 6 h. ( B – D ) Quantification of GCLC, GCLM, and NQO1 protein levels. ( E – J ) RT-qPCR analysis of the expression of antioxidant response element (ARE)-antioxidant genes ( E – G ), PSMC3 ( H ), PSMC4 ( I ), and XPC ( J ) following vehicle or iAs 3+ treatment ( n = 6). The data are presented as the mean ± SD; * p < 0.05, NRF1-742 -OE versus Cont -OE HaCaT cells; # p < 0.05, NRF1-772 -OE versus Cont -OE HaCaT cells.

Article Snippet: The antibodies against NRF1 (17062-1-AP), NQO1 (11451-1-AP), GCLC (12601-1-AP), KEAP1 (10503-2-AP), GCLM (14241-1-AP), tubulin (10094-1-AP), lamin A/C (10298-1-AP), and β-actin (60008-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR