Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: Comparative temporal toxicogenomic analysis of TCDD- and TCDF-mediated hepatic effects in immature female C57BL/6 mice.

doi: 10.1093/toxsci/kfn053

Figure Lengend Snippet: FIG. 4. Comparison of TCDD and TCDF temporal gene expression data. (A) Hierarchical clustering of the 116 differentially expressed genes differentially regulated by both TCDD and TCDF. This depiction provides a more qualitative assessment of the similarities of the gene expression of the 116 differentially expressed genes. (B) Correlation analysis of temporal gene expression and fold change significance value. It provides a more quantitative examination of the similarities in the expression patterns of the 116 differentially expressed genes by determining the Pearson’s correlation between the temporal gene expression (fold change) and significance (P1(t)) profiles of the 116 genes differentially regulated by both ligands. TCDD and TCDF paired data are plotted on a coordinate axis with the x-axis as the gene expression correlation and the y-axis as the significance correlation. A majority of the elicited responses fall into upper right quadrant identifying responses that are highly correlated between both gene expression and significance. There are also some genes that fall into lower right quadrant which represents genes with high correlation of expression but a lower correlation for significance.

Article Snippet: On the fourth day, animals were weighed and stock solutions of TCDD and TCDF (provided by The DOW Chemical Company, Midland, MI) were diluted in sesame oil (Sigma, St Louis, MO) to achieve the desired dose based on the average weight.

Techniques: Comparison, Gene Expression, Expressing

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: Comparative temporal toxicogenomic analysis of TCDD- and TCDF-mediated hepatic effects in immature female C57BL/6 mice.

doi: 10.1093/toxsci/kfn053

Figure Lengend Snippet: FIG. 6. QRT-PCR verification of microarray gene expression responses. The same RNA used for cDNA microarray analysis was examined by QRT-PCR. All fold changes were calculated relative to time-matched vehicle controls. Bars (left axis) and lines (right axis) represent data obtained by QRT-PCR and cDNA microarray data, respectively. The x-axis represents the time points. Genes are represented by official gene symbols and the QRT-PCR results are the average of four TCDD and five TCDF biological replicates. Error bars represent the SEM for the average fold change. The asterisk (*) represent a significant (p < 0.05) difference relative to time-matched controls for QRT-PCR.

Article Snippet: On the fourth day, animals were weighed and stock solutions of TCDD and TCDF (provided by The DOW Chemical Company, Midland, MI) were diluted in sesame oil (Sigma, St Louis, MO) to achieve the desired dose based on the average weight.

Techniques: Quantitative RT-PCR, Microarray, Gene Expression